Lab Exam 1

Question 1

_____ is a measure of how close two objects can be and still be distinguished.

Answer 1

Resolution

Question 2

Which of the following factors affect the resolving power of a microscope?

Answer 2

All of the above

Question 3

In Exercise 4 we focused on the enzyme malate dehydrogenase (MDH), which converted NADH to NAD+. Choose the terms below that correctly describe the roles of NADH in the MDH reaction.

Answer 3

• reduced form

- substrate

Question 4

When studying the abilities of two chemicals to permeate red blood cell membranes, chemical A had an initial absorbance of 0.32, which decreased at a rate of 0.2 absorbance units/second, and chemical B showed initial absorbance of 0.13 and with a decrease at 0.0008 absorbance units/sec. Which test substance was the fastest at penetrating the membrane?

Answer 4

Chemical B

Question 5

You are performing an experiment that requires lysis of red blood cells. What type of solution would you add to your cells in order to cause the cells to burst?

Answer 5

Hypotonic

Question 6

T/F: Microsomes are formed when endoplasmic reticulum membranes are broken into small pieces during homogenization of liver tissue.

Answer 6

True

Question 7

The hill reaction enables us to study the reducing power of chloroplasts by replacing NADP+ with ______, which absorbs light at 420 nm.

• NADH
• ferricyanide
• NAD+
• ferrischloride

Answer 7

ferricyanide

Question 8

You are conducting a study of protein concentration within different cell fractions, and you have made a dilution by combining 1 mL of your sample with 4 mL of water. This means that you will need to multiply your calculated value by ____ to account for your dilution.

Answer 8

x5

Question 9

When a P200 micropipette is set at “070”, it will dispense:

Answer 9

70 microliters

Question 10

Visual contrast of a sample can be increased by adjusting the ______ of your light microscope.

Answer 10

iris diaphragm

Question 11

The blank tube used in a spectrophotometric assay contains:

Answer 11

All reaction components EXCEPT sample

Question 12

Suppose the ocular of your microscope provides 5x mag, and the objective is 40x, what is the total mag of the object being viewed?

Answer 12

200x

Question 13

How many uL are there in 0.05 mL?

Answer 13

0.05x100=50

Question 14

What is the term used to describe the lysis of red blood cells?

Answer 14

hemolysis

Question 15

When plotting a graph of absorbance vs. protein concentration, you would expect to see a ________ relationship between two variables.

Answer 15

linear

Question 16

A 0.05 M solution is said to be ______ to a cell containing 0.15 M solute.

Answer 16

hypotonic

Question 17

How many nm is there in 1 mm?

Answer 17

10^6

Question 18

What is the name of the microscope part that enables you to focus with the 100x objective?

Answer 18

fine focus

Question 19

What is the general name of the instrument that can measure (detect) how much light is absorbed by a solution?

Answer 19

spectrophotometer

Question 20

What part of the microscope controls the amount of light that goes through the object being viewed?

Answer 20

condenser

Question 21

100 microliters is equal to _____ liters.

Answer 21

0.0001