lab diagnosis Flashcards
Oxidase Test
Blue means positive

Auramine-rhodamine (resist acid)

Antitoxin - serological
Vertical line - antitoxin
Horizontal Line - bacterium growth
Precipitin line - antibodies from paper strip and toxin produced by bacterial growth

Electron microscopy for viruses
Catalase Test
Bubbles mean positive

sugar fermentation
Detection of antibodies in patient serum
for chronic viral infection since antigen concentration in peripheral blood is low. Use ELISA

Fungi Cell Morphology (microscope)
Detecting antigens with animal antibodies
Identify serotypes - E Coli O157H7,
Strep Throat – extrant antigens and mix with strips that contain antibodies from animals against sterptococcus - color change – agglutination reaction

Negative stain

Fungi Colony Morphology (culture)
Parasites and their egg recognition
Microscopes
CPE
Identify viruses by using inclusion bodies or staining. Virus kills cells that are cultured.

Ziehl Neelsen stain (resists acid, for mycobacterium)
Leprosy
use armadillo to see if person has it.
PCR
DNA or RNA sequcnes for viruses or bacteria
Lowenstein-Jensen
culture mycobacterium tuberculosis
Urease Test
Pink is positive

Antibiotic Susceptibitlity - disc diffusion test

Biological with animals - C. Diff hard to culture
bacterial toxin

MacConkey agar
Selective and differential media
red - e.coli fermenting lactose
white - nonfermenting pathogens