Lab 4 & 5 Flashcards

1
Q

after using a microscope always?

A

leave lowest power objective 4x in place
remove the slide
clean the stage

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2
Q

Parfocal

A

objective lenses
- means once focused on an object at lower power , will only have to make minor fine focus adjustments when you increase the magnification by switching to a higher power objective lens

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3
Q

total magnification

A

determined by all the lenses through which it is viewed
objective lens x ocular lens (10x) = total magnification
4 x 10 = 40
10 x 10 = 100
40 x 10 = 400

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4
Q

inversion

A

what you the object as, as you look through the microscope

- refers to the image being upside down and reversed

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5
Q

moving the slide up and left what happens to image?

A

goes opposite way

moves down and right

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6
Q

the objective lenses are concentrically mounted so the image seen at the higher magnification is?

A

the middle of the image seen at the lower power

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7
Q

depth of focus

A

the vertical distance that remains in focus at one time

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8
Q

depth of focus

as the stage moves upwards (or the objective moves downward) which object come into focus first? top middle or bottom?

A

top

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9
Q

Diameter of field

A
  • the circle visible through the lens

- length across the lighted portion of the image from one edge to the other edge

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10
Q

the determination of the size of the Diameter of Field at each ojective power will help?

A

determine the approximate sizes of the specimens observed

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11
Q

1 mm = ? micrometer

A

1000 micrometer (um)

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12
Q

Objective Lens Magnification

4x

A

scanning lense

- red

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13
Q

Objective lens magnification

10x

A

low power lens

- yellow

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14
Q

Objective lens magnification

40x

A

High power lens

- blue

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15
Q

scanning magnification vs scanning diameter

A

4x = 40 = 5000 um

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16
Q

low power magnification (lpm) vs low power diameter (LPD)

A

10x = 100 = 2000 um

17
Q

High Power Magnificationn (LPM) vs High Power Diameter (HPD)

A
40x = 400 = 500 um
HPD = LPD x (LPM/HPM)
18
Q

does low power or high power have a larger field of view that allows you to see more of the object?

A

low power

19
Q

Which has a smaller field but magnifies to a greater extent?

A

high power

20
Q

wet mount

A

specimen suspended in liquid

21
Q

when drawing always label?

A

nucleus, cell membrane, and cytoplasm

22
Q

What do you use to clean lenses?

A

special lens paper

23
Q

plasma membrane

A

membrane at the boundary of every cell that acts as a selective barrier to the passage of ions and molecules into and out of the cell
- consists of phospholipid bilayer with embedded protein

24
Q

cell wall

A

a protective layer external to the plasma membrane in plant cells, bacteria, fungi, and some protists
- protects the cell and helps maintain its shape

25
Q

cytoplasm

A

the contents of a eukaryotic cell between the plasma membrane and the nucleus

  • consists of a semifluid medium and organelles
  • can also refer to the interior of a prokaryotic cell
26
Q

central vacuole

A

in a plant cell, a large membranous sac with diverse roles in growth and the storage of chemicals and wastes

27
Q

chloroplast

A

an organelle found in plants and photosynthetic protists that absorbs sunlight and uses it to drive the synthesis of organic molecules (sugars) from carbon dioxide and water

28
Q

contractile vacuole

A

??

29
Q

cilia

A

?

30
Q

Nucleolus

A

a structure within the cucleus where ribosomal RNA is made and assembled with proteins imported from the cytoplasm to make ribosomal subunits

31
Q

Nucleus

A
  1. an atoms central core containing protons and neutrons

2. . the genetic control center of a eukaryotic cell

32
Q

pseudopod

A

?

33
Q

flagella (plural)

flagellum

A

a long cellular appendage specialized for locomotion

- flagella of prokaryotes and eukaryotes differ in both structure and function

34
Q

amoeboid movement

A

move and feeds by means of pseudopodia

35
Q

each drawing should include

A

name of the organism, total magnification of the microscope and an estimate of the organism’s size

36
Q

to estimate size

scanning power field of view

A

= 4mm

37
Q

to estimate size

low power field of view is

A

1.8 mm

38
Q

to estimate size

high power field of view is

A

.45mm