Lab 32: Utilization of Malonate

Question 1

malonate test?

Answer 1

determines the ability of microorganisms to use malonate as the sole source of carbon and helps to distinguish Gram-negative bacteria Enterobacteriaceae and A. facecalis and Acinetobacter sp.

Question 2

what does malonate broth contain?

Answer 2

green prior to inoculation. contains sodium malonate (carbon source) and pH indicator bromthymol blue and glucose and yeast extract to support growth

Question 3

organisms that are able to use malonate produce?
-organisms that are unable to?

Answer 3

-alkaline metabolic byproducts that cause the broth to turn blue
-organisms unable to use malonate ferment the glucose instead which produces acid and turn the broth yellow
-no color change = neither malonate or glucose

Question 4

results
light to dark blue
yellow
green

Answer 4

light to dark blue = pos for use of malonate
yellow = fermented glucose only
green = neither