Lab 20: Casein Hydrolysis Test

Question 1

Casein hydrolysis tests for

Answer 1

production of exoenzyme caseinase by microorganisms

Question 2

TSA is seeded with?

Answer 2

sterile skim milk

Question 3

if casein is produced?

Answer 3

area of clearing (zone of hydrolysis) will occur surrounding the bacterial colony and indicates casein was degraded

Question 4

Casein hydrolysis results

Answer 4

Bacillus subtilis = positive for hydrolysis of casein
E. coli = negative for hydrolysis of casein

Question 5

gram designation of bacillus and escherichia?
pathogenic member of Bacillus and disease?
Escherichia belongs to which family?
two diseases of E.col?

Answer 5

-Bacillus is pos and Escherichia is neg
-B. anthracis which causes Anthrax
- Enterbacteriaceae
-Cholecytitis and Cholangitis