Lab 24: DNase Test

Question 1

this test for

Answer 1

to detect DNase activity of microorganisms and differenitates S. aureus from nonpathogenic staphylococci (Gram-positive cocci)

Question 2

what is DNase?

Answer 2

an enzyme that breaks down extracellular DNA. strong correlation between DNase activity and coagulase activity in pathogenic staphylococci

Question 3

DNase Test Agar with Methyl Green contains?

Answer 3

DNA, methyl green, and NaCl.

Question 4

clearing of the dye indiciates? aka clear zone
results for S. aureus and S. epidermis

Answer 4

positive for DNase activity
-S. aureus = positive
S. epidermis = negative