Lab 21: Starch Hydrolysis Test

Question 1

what does starch hydrolysis tests for?
-some bacteria and molds?

Answer 1

-hydrolysis of starch (polysaccharide) to maltose (disaccharide)
some bacteria and molds are able to produce amylase which diffuses into media immediately and degrades starch

Question 2

what is used after incubation of starch hydrolysis?

Answer 2

iodine solution
-blue-black reaction = starch is not hydrolyzed
-zone of hydrolysis = amylase was produced

Question 3

results for starch hydrolysis

Answer 3

E. coli = no amylase produced
B. subtilis = amylase produced

Question 4

members of Bacillus produce a dormant stage called?
members that also produce these structures?
species which causes botulism?
species which causes gas gangrene?

Answer 4

-endospore
-Clostridium
-Clostridium botulism
-Clostridium perfringes