Lab 25: Coagulase Production

Question 1

what is coagulase?
-may allow?
-useful in?

Answer 1

exoenzyme associated with pathogenic species of Gram-positive cocci such as Staphylococcus
-may allow bacterium to build a wall around itself for host’s immune system
-useful in diagnosis of several disease and differentiates of pathogenic species from nonpathogenic species of Gram-positive cocci

Question 2

what is coagulase specifically associated with?

Answer 2

Staphylococcus aureus that converts (soluble) fibrinogen in plasma to (insoluble) fibrin

Question 3

citrated plasma is made up of?

Answer 3

rabbit plasma with sodium citrate added to prevent spontaneous coagulation

Question 4

results for S. aureus
S. epidermis
control tube

Answer 4

pos
neg
neg