Lab 3 Flashcards
Culture media according to their physical state
Solid media
Semi-solid media
Liquid media
Consist mainly of substances mixed with a solidified agar, gelatine or albumin matrix
Solid Media
Are especially used for keeping stocks of bacteria for long periods of time. Agar is usually added in amounts of 0.5-1.0%
Semi-solid media
Are prepared without such substances as gelatine or agae
Liquid Media
Culture media according to their chemical composition
Synthetic Media
Non-synthetic media
Living tissue
One in which the chemical composition of the ingredients is known.
Synthetic media
Examples of synthetic media
Ringer’s solution and Locke’s solution
One in which the precise composition of some or all the nutritive elements used is not definitely known
Non-synthetic media
Example of Non-synthetic Media
Meat extract broth
One in which living tissue cells are present. These are especially used for the cultivation of ricketssiae, Chlamydia and viruses
Living tissue
Example of Living tissue culture media
HeLa cell lines
Primary Monkey Kidney cells
Embryonated eggs
Culture Media according to their use/functional type:
Simple media
Specific media
Enriched media
Differential media
Selective media
Transport media
Are ordinary media which are not enriched and in which non-fastidious organisms are easily cultivated
Simple meda
Are ordinary media which are not enriched and in which non-fastidious organisms are easily cultivated
Simple media
Examples of simple media
Nutrient broth
Nutrient agar
Are special media which are used for the cultivation of a particular organism
Specific media
Examples of Specific media
Loeffler’s Coagulated serum medium
Lowenstein-Jensen medium
Saboraud’s Dextrose Agar
Fletcher’s medium
Include 5% sheep blood agar, enriched chocolate agar and several infusion-based media, all of which may be further enriched by additives
Enriched media
These media must be carefully tested for sterility before they are used since even fastidious organisms are likely to grow
Enriched media
Example of Enriched Media
Blood agar
Chocolate agar
Contains dyes, sugars and indicators designed to elicit a characteristic biochemical response (usually a color) and are used to differentiate groups of organisms such as lactose fermenters and non-lactose fermenters
Differential Media
Examples of Differential media
MacConkey’s
EMB agars
Triple sugar iron
Mannitol salt agar
Contain, in addition to components found in differential media
Selective media
_____ and ______ may be classed as selective because they are somewhat inhibitory than MacConkey selective media also are capable of detecting H2S production
Hektoen enteric agar and Xylose lysine desoxycholate agar
Weigh –> Autoclave –> Dispense
Plate media
Weigh –> Dispense –> Autoclave
Tube Media
Composed of only one kind of microorganism
Pure culture
There are two or more organisms that have distinct characteristics and can be separated easily
Mixed culture
A method that prevents the introduction of unwanted organisms into an environment
Aseptic technique
