Lab 3

Question 1

Culture media according to their physical state

Answer 1

Solid media
Semi-solid media
Liquid media

Question 1

Consist mainly of substances mixed with a solidified agar, gelatine or albumin matrix

Answer 1

Solid Media

Question 1

Are especially used for keeping stocks of bacteria for long periods of time. Agar is usually added in amounts of 0.5-1.0%

Answer 1

Semi-solid media

Question 1

Are prepared without such substances as gelatine or agae

Answer 1

Liquid Media

Question 1

Culture media according to their chemical composition

Answer 1

Synthetic Media
Non-synthetic media
Living tissue

Question 1

One in which the chemical composition of the ingredients is known.

Answer 1

Synthetic media

Question 1

Examples of synthetic media

Answer 1

Ringer’s solution and Locke’s solution

Question 1

One in which the precise composition of some or all the nutritive elements used is not definitely known

Answer 1

Non-synthetic media

Question 1

Example of Non-synthetic Media

Answer 1

Meat extract broth

Question 1

One in which living tissue cells are present. These are especially used for the cultivation of ricketssiae, Chlamydia and viruses

Answer 1

Living tissue

Question 2

Example of Living tissue culture media

Answer 2

HeLa cell lines
Primary Monkey Kidney cells
Embryonated eggs

Question 3

Culture Media according to their use/functional type:

Answer 3

Simple media
Specific media
Enriched media
Differential media
Selective media
Transport media

Question 4

Are ordinary media which are not enriched and in which non-fastidious organisms are easily cultivated

Answer 4

Simple meda

Question 4

Are ordinary media which are not enriched and in which non-fastidious organisms are easily cultivated

Answer 4

Simple media

Question 5

Examples of simple media

Answer 5

Nutrient broth
Nutrient agar

Question 6

Are special media which are used for the cultivation of a particular organism

Answer 6

Specific media

Question 7

Examples of Specific media

Answer 7

Loeffler’s Coagulated serum medium
Lowenstein-Jensen medium
Saboraud’s Dextrose Agar
Fletcher’s medium

Question 8

Include 5% sheep blood agar, enriched chocolate agar and several infusion-based media, all of which may be further enriched by additives

Answer 8

Enriched media

Question 9

These media must be carefully tested for sterility before they are used since even fastidious organisms are likely to grow

Answer 9

Enriched media

Question 10

Example of Enriched Media

Answer 10

Blood agar
Chocolate agar

Question 11

Contains dyes, sugars and indicators designed to elicit a characteristic biochemical response (usually a color) and are used to differentiate groups of organisms such as lactose fermenters and non-lactose fermenters

Answer 11

Differential Media

Question 12

Examples of Differential media

Answer 12

MacConkey’s
EMB agars
Triple sugar iron
Mannitol salt agar

Question 13

Contain, in addition to components found in differential media

Answer 13

Selective media

Question 14

_____ and ______ may be classed as selective because they are somewhat inhibitory than MacConkey selective media also are capable of detecting H2S production

Answer 14

Hektoen enteric agar and Xylose lysine desoxycholate agar

Question 15

Weigh –> Autoclave –> Dispense

Answer 15

Plate media

Question 16

Weigh –> Dispense –> Autoclave

Answer 16

Tube Media

Question 17

Composed of only one kind of microorganism

Answer 17

Pure culture

Question 18

There are two or more organisms that have distinct characteristics and can be separated easily

Answer 18

Mixed culture

Question 19

A method that prevents the introduction of unwanted organisms into an environment

Answer 19

Aseptic technique