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Bacteriology > Biosafety And Quality Control In A Microbiology Laboratory > Flashcards

Biosafety And Quality Control In A Microbiology Laboratory Flashcards

1
Q

Chemical fume hood vs. Biosafety cabinet

Used for dangerous chemicals

A

Chemical fume hood

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2
Q

Chemical fume hood vs. Biosafety cabinet

Used for infectious biological agents

A

Biosafety cabinet

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3
Q

Chemical fume hood vs. Biosafety cabinet

Protects the user

A

Chemical fume hood

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4
Q

Chemical fume hood vs. Biosafety cabinet

Protects the user, the environment, and the material

A

Biosafety cabinet

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5
Q

Chemical fume hood vs. Biosafety cabinet

No HEPA filter

A

Chemical fume hood

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6
Q

Chemical fume hood vs. Biosafety cabinet

Must have HEPA filter

A

Biosafety cabinet

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7
Q

Chemical fume hood vs. Biosafety cabinet

Exhausts air outside the building

A

Chemical fume hood

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8
Q

A device that encloses a working area to protect workers from aerosol exposure and infectious disease agents.

A

Biosafety cabinets

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9
Q

the air that contains the infectious materials is sterilized, either by heat, UV light or passage through a high-efficiency particulate (HEPA) resistance filter.

A

Biosafety cabinets

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10
Q

Open-fronted type of cabinet with negative pressure (ventilated cabinets)

A

Class I cabinet

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11
Q

It allows room (unsterilized) air to enter the cabinet, circulate around the area and expose the material within; only the air to be exhausted is sterilized using a HEPA filter.

A

Class I cabinet

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12
Q

It is used for biosafety levels 2 and 3 agents

A

Class I cabinet

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13
Q

It is also known as the laminar flow BSC

A

Class II cabinet

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14
Q

The most commonly used BSC in a clinical microbiology laboratory (Class IIA)

A

Class II cabinet

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15
Q

Sterilizes the air using HEPA filter that flows over the infectious material and the air to be exhausted.

A

Class II cabinet

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16
Q

Class II cabinet is used in BSL ____ and ____ agents

A

2 and 3

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17
Q

types of Class II cabinet:

has fixed opening; 70%of the air is recirculated

A

Class II A

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18
Q

types of Class II cabinet:

variable sash opening; used for chemicals, radioisotopes and carcinogens.

A

Class II B

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19
Q

Provides the highest level of safety to the worker

A

CLASS III CABINET

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20
Q

The air coming into and going out of the cabinet is sterilized using HEPA filter and the infectious material within is handled with rubber gloves that are attached and sealed in the cabinet.

A

CLASS III CABINET

21
Q

It is used for BSL 4 agents.

A

CLASS III CABINET

22
Q

Not known to consistently cause disease in healthy adult humans, and of minimal potential hazard to laboratory personnel and the environment.

A

BSL-1

23
Q

Examples of BSL-1

A

Saccharomyces cerevisiae
E. coli K-12, and
non-infectious bacteria

24
Q

Moderate potential hazard to personnel and the environment. Includes
bacteria andviruses that cause mild disease to humans, or are difficult to contract via aerosol in a lab setting.

A

BSL-2

25
Q

Examples of BSL-2

A

Hepatitis A virus,
Streptococcus pyogenes,
Borrelia burgdorferi (Lyme disease),
Salmonella species

26
Q

Microbes there can either indigenous or exotic, and they can cause serious or potentially lethal diseasethrough respiratory transmission.

A

BSL-3

27
Q

Examples of BSL-3

A

Yersinia pestis (plague),
Mycobacterium tuberculosis,
SARS,
rabies virus,
West Nile Virus,
hantaviruses

28
Q

Dangerous and exotic, posing a high risk of aerosol-transmitted infections. Infections caused by t h e s e microbes are frequently fatal and without treatment or vaccines.

A

B S L - 4

29
Q

Example of B S L - 4

A

Ebola virus
Smallpox virus

30
Q

CATEGORIES OF POTENTIAL INFECTIOUS AGENTS OF BIOTERRORISM

These are agents that pose the greatest public health threat.

A

CATEGORY A agents

31
Q

CATEGORIES OF POTENTIAL INFECTIOUS AGENTS OF BIOTERRORISM

They are easily transmitted and highly infectious.

A

CATEGORY A agents

32
Q

Examples of CATEGORY A agents

A

smallpox, Bacillus anthracis and Francisella tularensis

33
Q

CATEGORIES OF POTENTIAL INFECTIOUS AGENTS OF BIOTERRORISM

These are agents with moderate morbidity and low mortality.

A

CATEGORY B agents

34
Q

CATEGORIES OF POTENTIAL INFECTIOUS AGENTS OF BIOTERRORISM

Not easily transmitted as category A agents

A

CATEGORY B agents

35
Q

Examples of CATEGORY B agents

A

Coxiella burnetti, Burkholderia pseudomallei, and Rickettsia.

36
Q

CATEGORIES OF POTENTIAL INFECTIOUS AGENTS OF BIOTERRORISM

These are the emerging pathogens

A

CATEGORY C agents

37
Q

Examples of CATEGORY C agents

A

viruses that causes yellow fever,
dengue hemorrhagic fever,
influenza
SARS.

38
Q

Donning of PPE

A

Hand hygiene
Gown
Respiratory protection
Eye protection
Gloves

39
Q

Doffing of PPE

A

Gloves
Eye protection
Gown
Respiratory protection
Hand hygiene

40
Q

Substances which singly or in combination have a significant threat or hazard to human health or to the environment and require special handling.

A

Hazardous wastes

41
Q

Qc monitoring:

Thermometer shouldbe calibrated ______

A

Periodically

42
Q

Thermometer that differ by >10C from the reference thermometer should be

A

disposed

43
Q

Thermometers should be checked ______ for the presence of gas bubbles.

A

Daily

44
Q

The percentage of carbon dioxide must be checked

A

Daily

45
Q

The speed or revolution per minute (rpm) must be checked _____ using a ______

A

Twice a year
Tachometer

46
Q

Culture Media Should be checked based on their performance and sterility., and records should be kept for at least ______

A

Two years

47
Q

Media that are not quality-controlled by the laboratory must still undergo observation for

A

Moisture
Sterility
Breakage, and
Appearance

48
Q

Reagents should be tested _____ with both positive and negative controls

A

Daily

49
Q

Uses specific strains of microorganisms from American Type Culture Collection (ATCC)

A

Antimicrobial Susceptibility

Bacteriology (13 decks)

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