Biosafety And Quality Control In A Microbiology Laboratory Flashcards
Chemical fume hood vs. Biosafety cabinet
Used for dangerous chemicals
Chemical fume hood
Chemical fume hood vs. Biosafety cabinet
Used for infectious biological agents
Biosafety cabinet
Chemical fume hood vs. Biosafety cabinet
Protects the user
Chemical fume hood
Chemical fume hood vs. Biosafety cabinet
Protects the user, the environment, and the material
Biosafety cabinet
Chemical fume hood vs. Biosafety cabinet
No HEPA filter
Chemical fume hood
Chemical fume hood vs. Biosafety cabinet
Must have HEPA filter
Biosafety cabinet
Chemical fume hood vs. Biosafety cabinet
Exhausts air outside the building
Chemical fume hood
A device that encloses a working area to protect workers from aerosol exposure and infectious disease agents.
Biosafety cabinets
the air that contains the infectious materials is sterilized, either by heat, UV light or passage through a high-efficiency particulate (HEPA) resistance filter.
Biosafety cabinets
Open-fronted type of cabinet with negative pressure (ventilated cabinets)
Class I cabinet
It allows room (unsterilized) air to enter the cabinet, circulate around the area and expose the material within; only the air to be exhausted is sterilized using a HEPA filter.
Class I cabinet
It is used for biosafety levels 2 and 3 agents
Class I cabinet
It is also known as the laminar flow BSC
Class II cabinet
The most commonly used BSC in a clinical microbiology laboratory (Class IIA)
Class II cabinet
Sterilizes the air using HEPA filter that flows over the infectious material and the air to be exhausted.
Class II cabinet
Class II cabinet is used in BSL ____ and ____ agents
2 and 3
types of Class II cabinet:
has fixed opening; 70%of the air is recirculated
Class II A
types of Class II cabinet:
variable sash opening; used for chemicals, radioisotopes and carcinogens.
Class II B
Provides the highest level of safety to the worker
CLASS III CABINET
The air coming into and going out of the cabinet is sterilized using HEPA filter and the infectious material within is handled with rubber gloves that are attached and sealed in the cabinet.
CLASS III CABINET
It is used for BSL 4 agents.
CLASS III CABINET
Not known to consistently cause disease in healthy adult humans, and of minimal potential hazard to laboratory personnel and the environment.
BSL-1
Examples of BSL-1
Saccharomyces cerevisiae
E. coli K-12, and
non-infectious bacteria
Moderate potential hazard to personnel and the environment. Includes
bacteria andviruses that cause mild disease to humans, or are difficult to contract via aerosol in a lab setting.
BSL-2
Examples of BSL-2
Hepatitis A virus,
Streptococcus pyogenes,
Borrelia burgdorferi (Lyme disease),
Salmonella species
Microbes there can either indigenous or exotic, and they can cause serious or potentially lethal diseasethrough respiratory transmission.
BSL-3
Examples of BSL-3
Yersinia pestis (plague),
Mycobacterium tuberculosis,
SARS,
rabies virus,
West Nile Virus,
hantaviruses
Dangerous and exotic, posing a high risk of aerosol-transmitted infections. Infections caused by t h e s e microbes are frequently fatal and without treatment or vaccines.
B S L - 4
Example of B S L - 4
Ebola virus
Smallpox virus
CATEGORIES OF POTENTIAL INFECTIOUS AGENTS OF BIOTERRORISM
These are agents that pose the greatest public health threat.
CATEGORY A agents
CATEGORIES OF POTENTIAL INFECTIOUS AGENTS OF BIOTERRORISM
They are easily transmitted and highly infectious.
CATEGORY A agents
Examples of CATEGORY A agents
smallpox, Bacillus anthracis and Francisella tularensis
CATEGORIES OF POTENTIAL INFECTIOUS AGENTS OF BIOTERRORISM
These are agents with moderate morbidity and low mortality.
CATEGORY B agents
CATEGORIES OF POTENTIAL INFECTIOUS AGENTS OF BIOTERRORISM
Not easily transmitted as category A agents
CATEGORY B agents
Examples of CATEGORY B agents
Coxiella burnetti, Burkholderia pseudomallei, and Rickettsia.
CATEGORIES OF POTENTIAL INFECTIOUS AGENTS OF BIOTERRORISM
These are the emerging pathogens
CATEGORY C agents
Examples of CATEGORY C agents
viruses that causes yellow fever,
dengue hemorrhagic fever,
influenza
SARS.
Donning of PPE
Hand hygiene
Gown
Respiratory protection
Eye protection
Gloves
Doffing of PPE
Gloves
Eye protection
Gown
Respiratory protection
Hand hygiene
Substances which singly or in combination have a significant threat or hazard to human health or to the environment and require special handling.
Hazardous wastes
Qc monitoring:
Thermometer shouldbe calibrated ______
Periodically
Thermometer that differ by >10C from the reference thermometer should be
disposed
Thermometers should be checked ______ for the presence of gas bubbles.
Daily
The percentage of carbon dioxide must be checked
Daily
The speed or revolution per minute (rpm) must be checked _____ using a ______
Twice a year
Tachometer
Culture Media Should be checked based on their performance and sterility., and records should be kept for at least ______
Two years
Media that are not quality-controlled by the laboratory must still undergo observation for
Moisture
Sterility
Breakage, and
Appearance
Reagents should be tested _____ with both positive and negative controls
Daily
Uses specific strains of microorganisms from American Type Culture Collection (ATCC)
Antimicrobial Susceptibility
