Lab 1 Flashcards
A device used to examine small objects or the fine and detailed structure of larger objects
Microscope
It is the primary tool of the microbiologist and knowledge of microscopy is essential to those engaged in studying microorganism
Microscope
One that makes use of single convex lens and has limited capacity of magnifying an object
Simple microscope
It is often used as an aid in dissecting multi-cellular organisms
Simple microscope
Make use of a system of lenses-the ocular lens and the objective lens
Compound microscope
Mechanical parts”
- Base
- Handle/Arm
- Draw tube
- Revolving nosepiece
- Adjustment knobs
- Stage
May be Y- or U- shaped stand that supports the microscope. This ensures that the microscope is planted firmly
Base
Where all other parts are connected-stage, objectives, and neck. It is used in holding the microscope.
Handle/Arm
Upper and smaller end of the body tube bearing the eyepiece or ocular
Draw tube
Circular structure at the lower end of the body tube to which the objectives are attached.
Revolving nosepiece
This is often covered by a circular structure called dust shield, which protect the objectives and the specimen from dust
Revolving nosepiece
This is often covered by a circular structure called dust shield, which protect the objectives and the specimen from dust
Revolving nosepiece
Circular structure at the lower end of the body tube to which the objectives are attached.
Revolving nosepiece
Two pairs of wheels attached to either side of the body tube.
Adjustment knobs
Kind of knob that has bigger wheels used to adjust the low power and scanning objectives
Coarse adjustment knob
Kind of know that has smaller wheels used for delicate focusing in connection with higher power and oil immersion objectives. It is also used to make specimen more clear and vivid
Fine adjustment knob
Square platform where specimen is placed for observations.
Stage
Under the Illumination and Magnification Parts
- Ocular or Eyepiece
- Illuminator
- Iris diaphragm
- Condenser
- Objectives
Found on the draw tube through which the operator peeps during actual focusing
Ocular or Eyepiece
Serve as a light source. The intensity of light is often adjustable with a switch.
Illuminator
What mirror is used when viewing specimen in bright or natural light conditions
Flat mirror
Found below the stage consisting of regularly arranged circular blades. Used to regulate a central opening to decrease or increase the light reflected on the object
Iris diaphragm
A lens found immediately beneath the hole of the stage. It is used to concentrate light rays on the specimen.
Condenser
Tube-like structures attached to the revolving nosepiece. This is often lenses are often equipped with a spring device to prevent damage to the lens in case it accidentally touches the specimens.
Objectives
The objectives are ____, means that the focus of the different objective lenses have been adjusted such that they occur at the same distance.
Parfocal
It is usually shorter than the other two objectives and it usually forms the general outline or wider portion of the object
LPO
It is longer than the LPO. It forms a bugger image of the object in focus and is used to examine living microorganisms suspended in drops of water.
HPO
In most cases it is used to enlarge specimens that are so small under LPO
HPO
It has the highest degree of magnification and is used to examine stained smear preparations of microorganisms using immersion oil as their medium
OIO
What mirror is used when there is a little light available
Concave mirror
Refers to the removal or destruction of all forms of life, including bacterial spores present on an item or equipment or in a solution
Sterilization
Refers to reduction or inhibition of microorganism which likely cause infection
Disinfection
Can be attained by heat (moist and dry), radiation, filtration, and chemical
Sterilization
Can be done mostly by chemical agents
Disinfection
Aims to inhibit or kill microbes by one or more modes of action that include reaction on the components of cytoplasmic membrane, denaturation of cellular proteins and alteration on properties of nucleic acids
Disinfection
