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Biology 1006 > Lab 06 > Flashcards

Lab 06 Flashcards

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1
Q

What is an assay

A

An assay is the collection of known and unknown concentrations of something and using the known concentrations to calculate the unknown concentrations

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2
Q

What type of assay did we use in this lab

A

We used a biuret assay which is a copper based protein assay with a working range from 5-160 mg/ml and turns a blueish-purple colour when reduced

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3
Q

What is the dependent variable

A

Absorbance

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3
Q

What is a chromogen

A
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4
Q

What is BSA

A

Bovine Serum Albumin is a protein concentration standard which is stable and low reactive and used for creating standard curves

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5
Q

What is the independent variable

A

Concentration of BSA

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6
Q

What dye do we use

A

Chromogen

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7
Q

What is a standard curve

A

A graph used to determine the concentration of a chemical based on known concentrations

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8
Q

What is the formula to make a concentration from a stock

A

C1V1= C2V2

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Biology 1006 (9 decks)

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