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Oral Microbiology!!! > L8/9-Oral Ecology & BioFilm All mixed together > Flashcards

L8/9-Oral Ecology & BioFilm All mixed together Flashcards

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1
Q

As long as these normal flora, bacterial biofilms are fed well and maintained properly what can be the outcome?

A

generally little problem

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2
Q

Basic Biofim Properties: complex ______ & ______ community of different microorganisms.

A

cooperating & competing

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3
Q

Basic Biofim Properties: Microorganisms are arranged by micro colonies that are attached together by protective ______.

A

matrix

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4
Q

Microorganisms in biofilms are more resistant to _______, _________, and _________.

A

antibiotics, antimicrobials, and host responses

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5
Q

What are the dominant bugs in biofilm plaque, even after some time and growth of the plaque?

A

Strep and Actinomyces

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6
Q

The increased inflammatory materials (e.g., FMLP, cell wall products, and probably most importantly ____) around the gingival crevice causes, at first, gingivitis and eventually a pseudo- pocket and then real pockets with the increased growth of gram-negative organisms, best suited for the ______, ______, ________ environment of the deeper pockets.

A

LPS…anaerobic, CO2 rich, asaccharolytic

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7
Q

What are the two main things normal flora compete and cooperate for?

A

Space and Nutrition

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8
Q

What are three traits of normal flora?

A

Site specific 2.difficult to clear 3.tend to recolonize

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9
Q

What is an interesting role of normal flora that is related to bleeding?

A

Normal Flora synthesize Vitamin K! No vit k, no clotting yo

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10
Q

What are the first two bugs to colonize a new baby if a vaginal birth has occurred?

A

Yeast (candida albicans) and Lactobacillus

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11
Q

What are the two main bugs to access a new baby once family starts kissing it?

A

Streptococcus Salivans (kissing)….Bacteriodes (fecal/anal transmission)

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12
Q

What is the event that changes microbial colonization in the baby? What are the 2 new bugs to take advantage?

A

Eruption of the teeth! Creates multiple sites for different colonies to form….1.Strep Sanguinis 2.Strep Mutans

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13
Q

What specific event catalyzes S. Mutans to colonize?

A

the eruption of the 2nd Molars!

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14
Q

Why does bacteria recolonize quickly even after a professional cleaning?

A

S. Salivarius and others are hanging out on the tongue and soft tissues and they go right back to where they were before the cleaning

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15
Q

Which is dependent on enamel surface? Which is not so dependent?–>S. Mutans vs S. Salivarius.

A

S. Mutans is dependent on the enamel surface…S. Salivarius is not

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16
Q

In general, Gram ___ bacteria are going to reside in the saliva, tongue, and supra gingival areas vs Gram ___ that will hang out in sub gingival areas.

A

G + supra…G - sub G

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17
Q

What are the two most common morphologies of supra gingival bacteria?

A

Cocci and branched Rods

18
Q

What are the two most common morphologies of sub gingival bacteria?

A

rods and spirochetes

19
Q

What is the oxygen relationship of supra gingival bacteria?

A

facultative & some anaerobes

20
Q

What is the oxygen relationship of sub gingival bacteria?

A

facultative and mostly anaerobes

21
Q

What is the energy source of supra gingival bacteria?

A

Carbs!

22
Q

What is the energy source of sub gingival bacteria?

A

Protein

23
Q

Which is more attached/adhered to the tooth, supra or sub gingival bacteria?

A

supra-firmly adhered to plaque…sub-adherence less pronounced

24
Q

What diseases are associated with supra gingival bacteria?

A

Caries & Gingivits

25
Q

What diseases are associated with sub gingival bacteria?

A

Gingivitis & Periodontitis…NO caries!!!!

26
Q

What pH is associated with Supra Gingival bacteria? Why?

A

slightly acidic (in the 6’s)…c/o carb food–>acid waste

27
Q

What pH is associated with Sub Gingival bacteria? Why?

A

slightly basic (in the 7’s)…c/o protein food–>sulfur/nitrogen waste

28
Q

Dental plaque is a ______.

A

biofilm

29
Q

Dr. Harold ___ developed a model to study the effects of poor oral hygiene (i.e. ‘not mowing the lawn’) and found that an ______ in anaerobic forms and rods, as well as an _______ total bacterial mass, which correlated with the appearance of _______. This was _______ after 21 days.

A

Loe….Increase….increase…gingivitis…REVERSABLE!

30
Q

In Dr. Loe’s model: what happens to aerobic bacteria amount at the end of 21 days?

A

It declines pretty substantially

31
Q

In Dr. Loe’s model: What happens to the amount of anaerobic bacteria after the 21 days?

A

Major increase

32
Q

In Dr. Loe’s model: what happens to facultative bugs over the 21 days?

A

a more mild decline

33
Q

In Dr. Loe’s model: What happens to the total amount of bugs over the 21 days? What happens to them on day 22 with brushing?

A

exponential increase…go away after brushing :)

34
Q

As plaque increases in complexity and density, a ‘_______’ develops, where some bacterial end products provide the ________ or ________ beneficial for other bacteria.

A

‘food chain’…substrate or conditions

35
Q

What are the three substrates and or conditions for the pioneer bacteria on the tooth?

A

O2, CHO, Saliva

36
Q

What is the nickname for the bacteria that displace the pioneers?

A

‘vigilante flora’

37
Q

So the S. Mutans and the Actinomyces produce lactate…Who is hooking a brother up by consuming that lest it rots the enamel? (2 bug types)… What do they produce?

A

Veillonella and Neisseria….Acetate and Formate (which then feed the bad guys…thanks for notin’)

38
Q

Now that our pioneer colony has been displaced and bad guys are starting to roll in SUB G…. What is the ecological term used for the system at hand? Review: what are the main two groups of bacteria here?

A

‘Climax Community’…Gram - rod and spirochetes

39
Q

What is the Vitamin K precursor made by Veillonella and then used as fuel for Sub G bacteria?

A

Men-a-di-one

40
Q

What are the three bad smelling products of the Sub G bacteria?

A

Thiamine, putrescine, and isobutyrate

Oral Microbiology!!! (12 decks)

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