L7: Extremophiles and Introduction to Metabolism Flashcards
What are 4 environmental factors that influence microbial growth?
Oxygen concentration
Temperature
Solutes and water activity
pH
What kind of solutes decrease the availability of water to microbes?
salts and sugars
Lower water activity is proportional to what?
higher solute concentration (hypertonic)
What’s an example of a hypotonic environment?
fresh water lake or stream
What’s an example of a hypertonic environment?
Dead Sea, Great Salt Lake, peanut butter
What’s special about the Berkeley Pit?
pH of 2
What’s special about the microbe from the Berkeley Pit?
It has the ability to kill ovarian cancer cells
What two things does metabolism need?
flow of energy (capacity to do work) and participation of enzymes
What is the energy currency of cells?
ATP
What are the components of ATP?
3 phosphate groups, a ribose, and an adenine (adenosine = ribose + adenine)
What results from the removal of a phosphate group of ATP?
large negative standard free energy change
What are 4 energy generating systems?
Aerobic respiration
Anaerobic respiration
Fermentation
Photosynthesis
How is ATP made during photosynthesis?
photophosphorylation
What does ∆G for a reaction determine?
how much energy is available to do work
What can be used to predict the direction of a reaction?
∆G
What’s the equilibrium constant formula?
Key = [products] / [reactants]
What units is ∆Gº’ expressed in?
kcal or kjoules/mol
How is Keq related to ∆G?
∆Gº’ = -2.303 RT logKeq
What’s favored when Keq > 1?
products
What’s favored when Keq < 1?
reactants
What’s the change in energy when Keq < 1?
energy is absorbed
What’s the change in energy when Keq > 1?
energy is released
What types of reactions are often coupled?
endergonic
What exergonic reaction often drives endergonic reactions?
ATP breakdown
What can result from redox reactions?
energy release, that can be used to form ATP