L6

Question 1

How many Americans have CKD?

Answer 1

37million

Question 2

How is organogenesis studied?

Answer 2

advances in molecular mapping & biological imaging

Question 3

What happens if the first mini-kidneys were ver 500 microns?

Answer 3

they formed a necrotic core

Question 4

What is the peptigel made from?

Answer 4

Gelatine

Question 5

How do you induce nephrogenesis?

Answer 5

Give SC cocktail of progenitors

Question 6

What do the self-assembling peptide hydrogels exhibit?

Answer 6

ECM fibre architecture & variable stiffness

Question 7

How do you check to see if the “kidneys” work?

Answer 7

Filtering dextran into lumen

Question 8

How do you reduce off target cells?

Answer 8

changing stiffness

Question 9

How is fidelity of kidney organoids improved?

Answer 9

spatial distribution

Question 10

How can you obtain UREC?

Answer 10

Urine sample collected
spin down urine for UREC
Colonies formed on day 9
Isolation of Type 1 & type II UREC

Question 11

What is the appearance of type 1 UREC?

Answer 11

Smooth edged & cobble stone edged
passaged upto P5

Question 12

What is the appearance of type II UREC?

Answer 12

slender, more randomly arranged
passaged up to P10

Question 13

How are URECs reprogrammed to iPSCs?

Answer 13

Using non intergrating episomal plasmids

Question 14

What do vectors for URECs contain?

Answer 14

oriP/EBNA-1 backbone delivering reprogramming factors (Oct4, Sox2, Nanog, Lin28, Klf4 & L-Myc)