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Cell products > L7 > Flashcards

L7 Flashcards

1
Q

What makes up the microenviroment?

A

Other cell types
Growth factors
ECM proteins

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2
Q

What does the ECM provide?

A

Signaling via adhesion molecules on ECM fibers binding cell receptors (integrins)
Mechanical support - influences behavior

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3
Q

What do organoid protocols focus on?

A

Signalling cues

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4
Q

What does signalling through growth factors do?

A

Signalling at defined times essential to inducing necessary cell types

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5
Q

What are 2 current approaches of ECM supports?

A

No support matrix
Animal-derived support matrix

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6
Q

What is the future approach of ECM supports?

A

Organoid encapsulated in ECM mimic

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7
Q

What is a biomaterial/

A

natural or synthetic material that is intended to interact with biological systems
In contact with body fluids continuously

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8
Q

What do biomaterials control?

A

Cell fate decisions & use in regenrative therapeutics

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9
Q

What is matrigel?

A

Murine-derived heterogenous complex of ECM proteins produced by Engelbreth-Holm-Swarm sarcoma cells

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10
Q

What ECM proteins are in a matrigel.

A

Laminin
Collagen IV
Entactin
Perlecan

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11
Q

What are 4 limitations of matrigel?

A

Undefined
50% batch-to-batch variability
Variable stiffness
Limit therapeutic progression

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12
Q

What is stiffness measured in/

A

Gā€™ elastic modulus - materials resistance to being deformed

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13
Q

What is PEG?

A

Polyethylene glycol - synthetic polymer

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14
Q

Does PEG support stem cell expansion?

A

No PEG is inert

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15
Q

What would stiff environment hinder?

A

growth & morphogenesis

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16
Q

What was the dynamic hydrogel composed of?

A

Combined stable PEG with hydrolytically degradable PED polymer with RGD & Laminin incorporation

17
Q

What does the dynamic hydrogel do to stiffness?

A

Initial high stiffness for stem cell maintenance & lower stiffness for cellular differentiation

18
Q

What proteins were decreased in stiffer gel in kidney type organoids?

A

WT1, LTL, VEGFR

19
Q

Does stiffness have a cell type specific effect?

A

yes - podocytes favour stiffer ECM

20
Q

What does growth induced compression impact?

A

Size of cerebral organoids

21
Q

What are neural rosettes/

A

Lumenized neuroepithelium during embryonic brain development

22
Q

What do kidneys organoids in SAPH reduce?

A

Off-target cells in comparison to Transwell

23
Q

Which environment were the Kidney cells better organised?

A

Stiffer environment

24
Q
A

Cell products (12 decks)

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