L5 Flashcards
Who carried out the first pancreatic transplant?
1966 by William Kelly & Richard Lilehei - duct-ligated segmental pancreas
What scientist found a way to use human stem cells to manufacture beta cells?
Doug Melton
How can beta stem cells be protected from the immune system?
Encapsulation
Modify cells to evade immune system
How does the pancreas evolve?
One dorsal & 2 ventral buds from primitative foregut
What are the 4 stages of pancreatic development?
Branching morphogenesis
NEUROG3 cells appear
formation of initial hormone cells
established lineage-committed mono-hormonal endocrine cells
What gene is the master regulator of beta cells?
PDX1
What are 2 soluble inductive signals?
Inhibitors
Recombinant
What does PDX1 control?
A whole identity program - control genes that are involved in chromatin remodelling & differentiation
What does PDX1 do during development?
Specifies early pancreatic epithelium permitting its proliferation, branching & subsequent differentiation
What is each step in differentiation is regulated by?
Stage specific transcription factors
What signalling is inhibited in the transition from primitive gut tube to posterior gut tube?
Hh signalling (cyclopamine added in invitro)
Were the SC beta similar to human beta cells?
yes they behave/look appropriate
