L16 GOK

Question 1

does agrin directly act with MUSk ?

Answer 1

no

Question 2

what type of molecule is agrin ?

Answer 2

heparin sulfate proteoglycan

Question 3

what is the tetracycline controlled expression system?

Answer 3

is a method of inducible gene expression where
transcription is reversibly turned on or off in the presence of the antibiotic tetracycline or one of its
derivatives (e.g. doxycycline).

Question 4

whats Tet-O ?

Answer 4

Tetrocycline responsive regulatory element

promotor that stimulated with presence of DOX/TET

Question 5

Tet-O is coupled to ?

Answer 5

Cre

Question 6

what protein is needed for DOX to bind Tet-O ?

Answer 6

reverse tetracycline-controlled

transactivator rtTA

Question 7

rTA is coupled to the tissue specific promotor what is the one used for muscle ?

Answer 7

human alpha1-actin gene - ACTA1

Question 8

how do you KO LRP4 conditionally in muscle ?

Answer 8

breed two mice
mouse 1 LRP4 floxed alleles - exon - lox P -exon - lox P - exon

mouse 2 - Tet-O-Cre - ACTA1-rTA

Question 9

What does α-Bungarotoxin do ?

Answer 9

irreversibly blocks nACHr

Question 10

when α-Bungarotoxin is coupled to rhodamine what does it do?

Answer 10

labels nACHr irreversibly

Question 11

what happens to the muscle in LRP4 KO mice

Answer 11

NMJs become fragmented

Question 12

KO of LRP4

Answer 12

reduces agrin
less active zone
loss of synaptic vesicles from MN terminals
retraction of post junctional folds