L1: INTRO TO MICROBIOLOGY Flashcards
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In the word microbiology, “micro” means
small
In the word microbiology, “bios” means
life
In the word microbiology, “logia” means
study
Scientific study of microorganisms, those being of unicellular (single-celled), multicellular (consisting of
complex cells), or acellular (lacking cells)
Microbiology
5 SUB DISCIPLINES of MICROBIOLOGY
BACTERIOLOGY
VIROLOGY
MYCOLOGY
PARASITOLOGY
IMMUNOLOGY
Prokaryotes consists of ___ & ___
bacteria & archea
Eukaryotes consists of ___ & ___
Plant cells & Animal cells
what is absent in prokaryotic cells but is present in eukaryotic cells
nucleus and cellular organelles
How does bacteria replicate
binary fission
Fungi and Parasites. Eukaryotic or Prokaryotic
Eukaryotic
bacteria came form the word
“baktēría”
“baktēría” means ____ or cane
staff
bacteria was first discovered due to its ___ shape
rod shape
singular term for bacteria
BACTERIUM
plural term for bacteria
BACTERIA
bacteria reproduce through
binary fission
bacteria size is measured through
micron or micrometer
size range of bacteria
0.1u to 18u
size of most pathogenic bacteria
0.u - 10u
bacteria cause most of ___ infection
human infection
how bacteria is named. ____ + _____ = specie
Genera + Specific name
The species name of bacteria should be written in ____
italic or capitalized first letter
invented compounding of lenses
Zacharias Janssen (1570-1638)
modified the basic design of the microscope
Robert Hooke (1635-1703)
First to discover the cell
Robert Hooke (1635-1703)
described the forms of fungi such as bluish mold on a piece of leather
Robert Hooke (1635-1703)
Antony van Leeuwenhoek is the Father of ____
Father of Microbiology
first to observe and to experiment with microbes
Antony van Leeuwenhoek (1632-1723)
inventing the process that bears his name, pasteurization
Louis Pasteur (1822-1895)
Discover the principle of immunization
Louis Pasteur (1822-1895)
known for the isolation of microorganisms causing Anthrax and Tuberculosis.
Robert Koch (1843-1910)
developed solid media for culturing bacteria and various techniques to isolate
bacteria.
Robert Koch
the Father of Antisepsis developed antiseptic surgical procedures
Joseph Lister (1827-1912)
first person to isolate bacteria (Bacillus lactis) in pure form in a liquid culture
form
Joseph Lister (1827-1912)
developed the most important staining method in 1884 to visualize bacteria
known as Gram Staining
Hans Christian Gram (1853-1938)
1928 discovered the antibiotic Penicillin which has been extensively used
since then
Alexander Fleming (1881-1955)
The concept of vaccination was invented by this British physician.
Edward Jenner (1749-1823)
non pathogenic organism, their natural habitat is dead organic matter
saprophytes
unicellular or metazoan organism living in or on an organism of another species (host) on the expense of the host
Parasites
Classic disease-causing pathogens
Pathogenic microorganisms
can cause disease in immunocompromised individuals given an “opportune” situation, frequently germs of the normal flora
Opportunists or facultatively pathogenic organisms
capacity of a pathogen species to cause disease
Pathogenicity
sum of the disease causing properties of a strain of a pathogenic species
Virulence
Time between infection and manifestation of disease symptoms: can be measures in hours, days, weeks or even years
incubation period
A parasitological term: time between infection and first appearance of products of sexual reproduction of the pathogen
Prepatency
the totality of host species “susceptible” to infection by a given pathogen
Infection spectrum
Smallest number of pathogens sufficient to cause an infection
minimum infective dose
Method or pathway used by pathogen to invade host
mode of infection
microbiological presence of microorganisms on objects, in the environment, or in samples for analysis
contamination
presence of microorganism on skin or mucosa; typical of normal flora
colonization
invasion of a host organism by microorganisms, proliferation of the invading organisms and host reaction
infection
infection without outbreak of clinical symptoms
inapparent/sub-clinical INFECTION
infection with outbreak of clinical symptoms
infectious disease/ clinical infection
frequency of clinical manifestation of an infection in disposed individuals
probability of manifestation
infection arising from the colonizing flora
endogenous infection
infection arising from invasion of host by microorganisms from sources external to it
exogenous infection
infection acquired during hospitalization (UTI, infections of the respiratory organs, wound infection, sepsis)
nosocomial infection
infection that remains restricted to the portal of entry and surrounding area
local infection
lymphogenous and/or hematogenous spread of invading pathogen from the portal of entry: infection of organs to which pathogen shows a specific affinity
generalized infection
generalized infection three stages
incubation, generalization, organ manifestation
6 Factors Affecting Growth of Bacteria
Air - O2, CO2
Temperature
pH
Light
Osmotic Pressure
Nutrients
bacteria that needs oxygen to live
Aerobic Bacteria
3 types of aeroebic bacteria/aerobes
Obligate aerobes
Facultative aerobes
Microaerophilic bacteria
bacteria which can grow only in the present of oxygen
Obligate aerobes
example of obligate aerobes
P. aeruginosa
bacteria which are ordinary aerobes but can also grow without oxygen
Facultative aerobes
E. coli is what type of aerobe?
facultative aerobe
those bacteria that can grow in the presence of low oxygen and in the presence of low (4%) concentration of carbon dioxide
Microaerophilic bacteria
example of microaerophilic bacteria
Campylobacter jejuni
can live without 02, lack superoxide dismutase and catalase hence oxygen is lethal to these organisms
anaerobic bacteria
bacteria that can grow only in the absence of oxygen
obligate anaerobes
example of obligate anaerobes
Clostridium sp
the organisms that require higher amounts of carbon dioxide (CO2) for their growth are called
capnophilic bacteria
capnophilic bacteria grow well in the presence of ____ CO2 and ____ O2
5-10% CO2 and 15% O2
ex. of capnophilic bacteria
H. influenzae, Brucella abortus
optimum temp for bacteria
37°C
These bacteria are cold loving microbes that grow within a temperature range of 0-20°C. Most of soil and water saprophytes belong to this group.
Psychrophiles
Psychrophiles example
Arthrobacter sp,
Psychrobacter sp
These are moderate temperature loving microbes that grow between 25°C and 40°C. Most of pathogenic bacteria belong to this group
Mesophiles
These are heat loving microbes. They can grow at a high
temperature range of 55°C–80°C. B. stearothermophilus is an example.
Thermophiles
Most pathogenic bacteria grow between pH ___ and ___
7.2 and 7.6.
Very few bacteria, such as ____, can grow at acidic pH below 4.0.
lactobacilli
____ is an example of the bacteria that can grow at an alkaline (8.2–8.9) pH.
V. cholerae
Depending on the source of energy bacteria make use of, they may be classified as
Phototrophs and Chemotrophs
bacteria deriving energy from sunlight
Phototrophs
bacteria deriving energy from chemical sources
Chemotrophs
Organisms requiring high osmotic pressures are called
OSMOPHILIC BACTERIA
Sudden exposure of bacteria to hypertonic solution may cause osmotic
withdrawal of water, leading to osmotic shrinkage of the protoplasm which is called
plasmolysis
On the other hand, sudden transfer of bacteria from concentrated solution to
distilled water may cause excessive imbibition of water leading to swelling and bursting of cell
plasmoptysis
beneficial uses of bacteria
Decay and Decomposition
Soil fertility
Industrial production (dairy products, vinegar fermentation, probiotics)
Medicines (Antibiotics, vaccines, serums)
Genetic Engineering and Biotechnology
2 types of immunity
innate immunity
adaptive/acquired immunity
2 types of innate immunity
specific and non specific
2 types of adaptive/acquired immunity
active and passive