Kirby-Bauer Disk Diffusion Test Flashcards
1
Q
Kirby-Bauer Disk Diffusion Test
A
- all labs that do this test must follow same standards
- Standardized procedure for determining bacterial resistance
2
Q
Media Used for Kirby-Bauer Disk Diffusion Test
A
Mueller- Hinton Agar (must be 4 mm deep)
3
Q
The time plates are incubated for Kirby-Bauer Disk Diffusion Test
A
18-24 hours
4
Q
Temperature of incubation for Kirby-Bauer Disk Diffusion Test
A
35 +- 2 Degrees C
5
Q
Amount of Bacteria needed for Kirby-Bauer Disk Diffusion Test
A
0.5 McFarland Turbidity Standard
150 million bacterial cells per mL of liquid
6
Q
Age of Culture for set up for Kirby-Bauer Disk Diffusion Test
A
2-6 hours old
7
Q
Mechanism of Drug Action: How Antibiotics Work
A
- inhibition of cell wall synthesis
- inhibition of protein synthesis
- inhibition of structures and functions of DNA + RNA
- blocks a key metabolic pathway
- breakdown of the cell membrane function or structures
