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Microbiology Lab > Acid Fast Bacteria > Flashcards

Acid Fast Bacteria Flashcards

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1
Q

Acid Fast Bacteria

A

complex layered cell wall containing mycolic acid and waxes
(Acid fast cells have a waxy cell wall; mycolic acid in the cell walls of acid fast bacteria makes these organisms resistant to desiccation (drying) and disinfectants
-Only 2 Genera that are acid fast:
1. Mycobacterium
-diseases include Tuberculosis and Leprosy
2. Nocardia
-Pulmonary Nocardiosis (disease similar to Tuberculosis)

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2
Q

2 Genera that are acid fast

A
  1. Mycobacterium
    - diseases include Tuberculosis and Leprosy
  2. Nocardia
    - Pulmonary Nocardiosis (disease similar to Tuberculosis)
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3
Q

Acid Fast Stain- Ziehl-Neelsen (hot method)

A

This technique uses heat to force the stain through the resistant cell wall

  • Primary stain: Carbol Fuchsin (5 minutes on a hot plate)
  • Decolorizer: Acid alcohol
  • Counterstain: Methylene Blue (3 minutes)
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4
Q

Acid Fast Bacilli are stained what color?

A

RED

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5
Q

Acid Fast Bacilli can arrange as..?

A
  1. Pallisades (bacilli next to each other)

2. Cording (spreading bacilli)

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6
Q

Non-acid Fast microorganisms are stained what color?

A

BLUE

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7
Q

Acid Fast Stain- Ziehl-Neelsen (hot method) Explained

A
  • Primary Stain: Carbolfuchsin
  • Decolorizer: Acid Alcohol
  • Counterstain: Methylene Blue
  • Examination: Brightfield microscope 1000x examine 300 fields
  • Acid Fast: Dark red to pink bacilli
  • Non Acid Fast: Blue microorganisms
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8
Q

Acid Fast Stain- Kinyoun (cold method) explained

A
  • Primary Stain: Carbolfuchsin
  • Decolorizer: Acid Alcohol
  • Counterstain: Brilliant Green
  • Examination: Brightfield microscope 1000x examine 300 fields
  • Acid Fast: Dark red to pink bacilli
  • Non Acid Fast: Green microorganisms
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9
Q

Acid Fast Stain- Fluorochrome

A
  • Primary Stain: Auramine O or Auramine O- Rhodamine
  • Decolorizer: Acid Alcohol
  • Counterstain: Potassium Permanganate
  • Examination: Fluorescent microscope 150x-200x examine 30 fields
  • Acid Fast: yellow-green fluorescence or reddish orange fluorescence
  • Non Acid Fast: No fluorescing organisms
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10
Q

Acid Fast Stain- Ziehl-Neelsen results

A
  • Acid fast bacilli- Bright red or fuchsia color

- Non Acid Fast Cells- Blue

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11
Q

Acid Fast Stain- Kinyoun (cold method)

A

This technique uses a detergent to force the stain through the resistant cell wall

  • Primary Stain: Carbol Fuchsin with detergent (5 minutes)
  • Decolorizer: Acid Alcohol
  • Counterstain: Brilliant Green (3 minutes)
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12
Q

Acid Fast Stain- Kinyoun results

A
  • Acid Fast bacilli- bright red or fuchsia color

- Non Acid Fast- Green/Blue

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13
Q

Sputum Collection

A

For performing the Acid Fast Stain

  1. Clear your mouth
  2. Breath in and out 3 times
  3. Give a sputum sample
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Microbiology Lab (19 decks)

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