investigating enzymait reactions

Question 1

what does the enzyme amylase do

Answer 1

catalyses the break down of starch to maltose

Question 2

how can you easily detect starch

Answer 2

using iodine solution

Question 3

if starch is present what colour does the iodine change to

Answer 3

from a browny-orange to a blue-black

Question 4

how can you investigate how pH affects amylase activity

Answer 4

1) put a drop of iodine solution into every well of a spotting tile

2) heat a beaker of water with a bunsen burner

3) use a syringe to add 1cm3 of amylase solution and 1cm3 of buffer solution

4) wait 5 minutes

5)use a different syringe to add 5cm3 of starch solution to the boiling tube

6)immediately mix the contents of the boiling tube and start a stop watch

7) repeat the whole experiment with buffer solutions of different pH values

Question 5

how do you calculate the rate of reaction

Answer 5

rate = 1000/time