Introduction to Histology Flashcards
Flow diagram of cell -> to body?
-Cell
-Simple tissue
-Compound tissue
-Organ
-Organ system
-Body
What will show up as dark on e- microscopes?
e- dense items - as are stained w/ heavy metals
How are tissues prepared?
-Fixation - preserving tissue (formalin/glutaraldehyde)
-Dehydration (remove water) & cleaning (remove alcohol)
-Embed in suitable material (e.g., paraffin)
-Cut into thin slices
-Mount on glass slide
-Stain/dye cells/tissues (to see structures)
What does haematoxylin stain show & why?
-Binds to acids as the stain = base
-So e.g., - DNA, RNA = stained dark blue/purple
-These items = basophilic (base loving)
What does eosin stain show & why?
-Binds to bases as stain = acid
= cytoplasmic components = stained pink
-These items = acidophilic (acid loving)
What are the 3 embryonic/germ tissue layers?
-Ectoderm
-Endoderm
-Mesoderm
What are the 4 tissue types?
-Epithelial tissue = from ectoderm & endoderm (can also be mesoderm)
-Muscular tissue = from mesoderm
-Nervous tissue = from ectoderm
-Connective tissue = from mesoderm
What are 2 epithelial types that ectoderm & endoderm form?
-Ectoderm = squamous epithelium
-Endoderm = glandular
Role of epithelium x6?
-Protection (underlying tissues)
-Transcellular transport (across epithelial sheets)
-Secretions from glands (mucus, hormones, enzymes)
-Absorption from lumen
-Selective permeability - controls movement of materials between body compartments
-Detecting sensations
2 types of epithelia?
-Simple = 1 layer of cells
-Stratified = many cell layers
How does epithelia gain nutrients - as is avascular?
From connective tissue beneath - has blood vs - so pass though basement memb to ep
Layers from epithelia down?
-Epithelia
-Basement memb
-Connective tissue
What is the basement memb made up of?
-Basal lamina = secreted by ep
–> cells made of lamina lucida (lighter) & lamina densa (darker)
-Reticular lamina
Role of basement memb?
-Give support & attachment to ep
-Selectively permeable barrier (diffusion purposes)
3 types of simple epithelia?
-Squamous (flat)
-Cuboidal
-Columnar