Introduction Flashcards

1
Q

Why parasitology

A

Daily (or almost daily)
Major global animal and human health problems
Clinical disease
Subclinical disease/reduced production
Aesthetics/human animal bond
Animal welfare
Many zoonoses; public health
$

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2
Q

Impacts of perasites against host animal

A

Compete for nutrition
Mechanical obstruction
Suck or consume body fluids
Feed on or destroy solid tissues
Pressure atrophy
Allergic reactions
Produce toxic substances
Carry pathogens including other parasites
Reduce hosts resistance to other disease and parasites

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3
Q

How do parasites compete for nutrents

A

By ingesting intestinal contents- ascarids (roundworms)
Ab absorbing them through body walls- tapeworm

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4
Q

Mechanical obstruction can affect the host where

A

Of the intestines- ascarids
Of bile ducts- ascarids, trematodes
Of blood vessels- canine heartworm
Of bronchi or trachea- lung worms
Of lymph channels- filarial nematodes

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5
Q

What parasites suck or consume body fluids

A

Blood- mosquitos, hookworm
Lymph- midges
Exudates- lungworms

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6
Q

How do parasites feed on or destroy solid tissues

A

Directly- giant kidney worms, liver flukes
After liquefying them- chiggers

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7
Q

What type of allergic reactions can parasites cause

A

Hypersensitivity, scar tissue
Pig livers
Black flies, mosquitoes, fleas

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8
Q

What toxic substances can parasites produce

A

Hemolysins, histolysins (break down tissue), anticoagulants

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9
Q

What can parasites carry

A

Mosquitoes- malaria, heartworm, WEE and WNV
Dog flukes- ‘salmon poisoning’

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10
Q

How does entry and/or migration hurt the host

A

Creeping eruption
Cutaneous larval migrans
Sarcoptic mange

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11
Q

What does the VT do for parasites

A

Diagnosis and prognosis
Control and prevention
Treatment
Public health
Client education
COMMUNICATION

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12
Q

A functional definition of parasitism

A

A parasite is a smaller organism that lives within or on another, generally larger, organism, the host
The parasite causes some degree of harm to the host. This “harm” ranges from mild to severe
The parasite absolutely requires the host to complete its life-cycle

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13
Q

Parasite types

A

Macroparasites
Helminths and arthropods
Microparasites
Protozoa, bacteria, fungi and viruses

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14
Q

Nematodes are

A

roundworms, hookworms, pinworms, whipworms, etc
Toxocara canis- dog roundworm

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15
Q

Helminths are

A

Worms

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16
Q

Types of flatworms

A

Cestodes- tapeworm
Diphyllobothrium spp.
Tapeworm of fish eating vertebrates
Trematodes- flukes
Fascioloides magna- giant liver fluke of ruminants

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17
Q

Types of Arthropods

A

Bugs
Flea
Flies
Lice
Mites
Ticks

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18
Q

Examples of protozoas

A

Amoeboids
Ciliates
Flagellates
Sporozoans
Apicomplexans (coccidia)

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19
Q

Definitive host

A

Required for all parasites (need host to survive or reproduce)
Adult parasites are in the DH
Parasites undergoes sexual reproduction inside DH
Sometimes asexual reproduction in the DH

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20
Q

Intermediate host is

A

Required for many parasites (indirect life cycles)
Parasite undergoes development within IH
Never sexual reproduction

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21
Q

Paratenic host (PH)

A

helpful but not required
PH- infection occurs bu no reproduction, no development

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22
Q

Transport host

A

helpful but not required
No infection, simply transport (mechanical vector)

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23
Q

Shed stage is

A

Shed stage- and infection stage
Shed stage is what leaves the infected host (egg, larva, cyst etc.)

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24
Q

Infective stage is

A

Infective stage is what is infective to the next host- usually takes time in the enviro for a shed stage to become in infective stage

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25
Prepatent period is
(PPP)(infected but not shedding detectable numbers of shed stages- eggs or larvae etc)
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Patent period is
infected and shedding detectable numbers of shed stage
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Prevalence is
%infected in a population) and intensity( how many parasites in an infected animal
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How to acquire parasites
Ingestion of infective stages from a contaminated environment Ingestion of infective stages in infected intermediate hosts, often regular prey Skin penetration by infective stages Inoculation of infective stages by infected arthropod vectors Transfer of infective stages by direct contact with other infected hosts Transmammary infection of larvae from mother to offspring Other
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Nematodes are
(roundworm) Parasitic and free-living species Tiny (<1mm) to enormous (40cm) Genders separate (dioecious), sexual reproduction within DH Eggs or larvated eggs, or larvae (diagnosis; finding these stages) Direct and indirect life cycles Live in many hosts and tissues twitch host and tissue specificity Larvae of some species undergo complicated migrations
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Oviparous is
female nematode that passes eggs in the single cell stage or the morula stage (solid mass of cells, clustered together)
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Ovoviviparous is
female nematode that passes eggs with a first stage larva contained (and visible)
32
Larviparous is
female nematode that retains the eggs within the uterus till they mature and hatch and then pass live first-stage larvae
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How can nematodes infect hosts
Ingestion of infective eggs or larvae from the environment, contaminated food or water Ingestion of larvae within intermediate host or paratenic host Skin penetration by infected larvae Inoculation of infective larvae from an arthropod vector Prenatally from mother to fetus Through the mammary gland from mother to suckling young Each species of nematode has its own route(s) of infection
34
How to identify nematodes
Identification of nematode adults requires details such as host, size of worm, morphological features of the worm, usually on the head and tail ends Egg morphology is more commonly used for diagnosis, this also varies, for example, the presence or absence of larvae in the egg, size, and other morphological features (operculum, polar plugs, color, etc,.)
35
How to diagnose parasites
Fecal Flotation Helminth eggs (most), some protozoal oocysts Baermann larval sedimentation- live larvae in fresh feces submerged in water wiggle out and sink PCR and antigen based methods of detection and identification of parasites
36
Cestodes (segmented flatworms)- basic features
All species parasitic Some tiny (2mm), some enormous (12m) Hermaphrodite All have an indirect life cycle with intermediate host(s) Adults in DH parasitize the GI system, cause few problems and produce eggs immediately infective for the IH Larval stages in tissues of IH may cause serious problems Asexual reproduction may occur in IH depending on species
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Cestode structure
Have segmented sections immature segments--> mature segments--> gravid segments Segment = proglottid
38
What type of lifestyle does a cestode have
indirect
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Diagnostic parasitology for cestode
Fecal float
40
Trematode (leaf shaped flatworm)- basic features
All parasitic From tiny (<1mm) to giant (10cm) Hermaphroditic (veterinary importance) Sexual reproduction occurs in DH All have an indirect life cycle with intermediate host(s) First IH always a gastropod (slug or snail) Asexual reproduction in gastropod IH Adults in DH parasitize GIT or lungs; sometimes pathogenic Larval stages in IH parasitize many tissues; rarely pathogenic
41
Trematode transmission to the definitive host by
Ingestion of infective metacercariae on vegetation Ingestion of infective metacercariae in intermediate hosts Skin penetration by cercariae Through mammary gland from mother to suckling young Each species of trematode has its own route of infection
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Diagnostics for trematode
Fecal sedimentation For eggs too dense to float Primarily trematode eggs
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Arthropods- important groups
Arachnida Insecta
44
What are included in arachnida
Mites and ticks (acari)
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What are included in insecta
Bugs (hemiperta) Lice (mallophaga and anoplura) Fleas (siphenoperta) Flies (diptera)
46
Mites are
Dorso-ventrally flattened Six legs as larva Eight legs as nymph and adult Variable host specificity Microscopic “Hariry”
47
How to tell the difference b/w a burrowing and surface mite
Burrowing mites Short legs Surface mites Long legs
48
Mites basic life cycle
adults --> eggs--> larvae--> nymphs Life cycle complete in about 10-14 days Note: generally (but not always) the entire life-cycle is on the host
49
Ticks are
Dorso-ventrally flattened Six legs as larva Eight legs as nymph and adult Not very host specific Macroscopic Smooth Time to complete life cycles highly variable, depending on species and environment
50
Two types of ticks
Hard ticks Ixodidae Soft ticks Argasidae
51
Life cycle stages of ticks
Egg→ larva (3 pairs of legs) → nymph (4 pair of legs) → adult All stages require a blood meal to develop to the next stage
52
One-host ticks life stage
attach first as larvae, feed and moult to a nymph, feed and moult to an adult and fall off the original animal as adults and lay eggs in the environment- weeks to months later
53
Two-host ticks life stages
Attach on host 1 as a larva, feed and moult to a nymph on the host. Feed as a nymph and fall off the host 1. Moult to an adult in the environment and then find a new host as an adult. Mate and fall off into the environment to lay eggs.
54
Three-host ticks life stages
attach to host 1 as a larva, feed fall off and moult to a nymph, attach to the host 2 as a nymph, feed and fall off into the enviro. Moult to an adult. Attach to host 3 and feed and mate on the third host. Fall off into the enviro to lay eggs They life cycle of a three host tick may take 2 or 3 years or longer depending on host availability
55
Insect characteristics
Three pair of legs Three distinct body regions (head, thorax, abdomen) Head: two eyes, two antennae, and complex mouthparts Thorax: six legs and anywhere from zero (i.e fleas and lice) to four wings (i.e. beetles and some bugs) Abdomen: Reproduction eggs or larvae
56
Bugs are and the two types
Dorso-ventrally flattened Six legs Variable host specificity Cimidae -Bed bugs Reduviidae -Assassin bugs -Kissing bugs -Cone-noses
57
Life cycle of bed bugs- Cimex lectularius
Adults (on host only to feed)--> eggs in the enviro--> nymphs on host only to feed (blood feed)
58
Lice are
Dorso-ventrally flattened Six legs Quite host specific Mallophaga Chewing/biting lice Head wider than thorax Anoplura Sucking lice Head narrower than thorax
59
Fleas characteristics
Laterally flattened Six legs Not host specific
60
Types of flies
Nematocera Brachycera Cyclorhapha Time to complete life cycle highly variable, depending on species and enviro
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Nematocera flies types
Mosquitos Blackflies Midges
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Brachycera flies are
Horseflies Deerflies
63
Cyclorhapha flies are
Faceflies Hornflies Botflies
64
Arthropods can harm the animal host through several means
Annoyance- decreased feeding sleep and poor hair coat Blood loss- lice can drop a PCV to <10% Produce venoms and toxins- tix paralysis (ascending flaccid paralysis), spider bites Dermatosis (skin disease)- mange mites Miasis (infection of the flesh of the living animals with fly larvae)- cattle warbles Allergic reactions- flea dermatitis, dust mites (serious allergies in people), anaphylaxis from death of warbles in cattle Vectors for disease agents Mechanical vectors (transport hosts)- flies carry Trypanosomes, etc Biological vectors(intermediate hosts)- mosquitoes carry heartworm, fleas carry Dipylidium caninum
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Diagnosis of arthropods
Finding adults or immature stages and identifying them (as boat as one can- e.g. fleas) Sometimes symptoms Sometimes test treatment
66
Protozoa is
Single-celled, eukaryotic organisms Approximately 64 000 species described of which only 7 000 are parasitic to animals. The rest are free-living Protozoans infect a variety of tissue sites within the definitive host
67
The most common sites for protozoal detection
Blood samples- hemoprotozoa Most are found in the erythrocytes within a stained blood smear Ticks and blood sucking insects often serve as the IH and transmit RBCs containing the hemoprotozoa Can also find antigens or antibodies in blood samples Fecal samples- intestinal protozoa Tissue samples
68
Zoite means
body
69
Trophozoite is
the stage of the protozoan life cycle that is capable of feeding, movement and reproduction; often too fragile to survive transfer to a new host
70
How do protozoas transmit
Transmission to a new host most often occurs when the protozoan is in the cyst state or oocyst stage Most metabolic functions are suspended when the parasite is encysted Cyst wall prevents desiccation and protects it from other enviro damage
71
Locomotor organelles are and used for
Used to help identify the type of protozoa recovered from an animal Flagella Cilia Pseudopodia "false feet"
72
Flagella on protozoa is
Long, whiplike structures Single or multiple Flagellates Trichomonads, Leishmania, Giardia Sometimes the flagellum passes backwards along the cell, joined at a few or several points and is thus termed an undulating membrane (Genus Trypanosoma)
73
Cilia on protozoa are
Short flagella, usually arranged in rows or tufts Typically found in various arrangements around the mouth and/or rural groove of the ciliates such as Balantidium coli and Ichthyophthirius multifiliis
74
Pseudopodia “false feet” on protozoa are
Temporary extensions and retractions of the body wall to surround potential food particles or for movement Typical of amoebas such as Entamoeba histolytica
75
Protozoa of vet importance
Flagellates Mucosoflagellates Direct life cycle E.g giardia (Direct life cycle) Tritrichomonas spp. (direct life cycle) Histomonas (indirect life cycle) Hemoflagellates Arthropod-transmitted E.g. Trypanosoma Ciliates Direct life cycles Asexual reproduction (binary fusion) E.g. Balantidium coli in swine (rarely zoonotic) E.g. Ichthyophthirius multifiliis in fish (ich) Sporozoa/apicomplexans Coccidia Direct life cycles (GIT) E.g. Eimeria Indirect life cycles (tissue cysts) E.g. Toxoplasma Haemosporidia Arthropod-transmitted E.g. babesia Amoebae Direct life cycle, asexual reproduction Free-living Amoeba (humans, dogs) Acanthamoeba spp. Naegleria fowleri
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Apicomplexans: coccidia
Direct (D) or indirect(I) life cycles Eimeria D Cystoisospora D/I Cryptosporidium D Toxoplasma I Neospora I Sarcocystis I Eimeria spp. – coccidia of ungulate
77
Problems caused by protozoal infections
Diarrhea Fevers and flu-like symptoms Neurological signs Abortions Skin lesions
78
Diagnosing protozoa
Appearance on fecal smear/flotation Fecal smear- simple, quick, low sensitivity False - are common Protozoal trophozoites (esp. If symptomatic) and cyst Fecal flotation Various flotation solutions Protozoal oocysts (Coccidia, sarcocytes) Immunofluorescent tests Immunohistochemistry Serology Molecular detection and identification
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