Intro to Hematology Flashcards
What questions do you ask yourself before sample collection
-What specific tests are needed?
-How much sample do I need?
-What equipment/tubes are appropriate?
-How quickly will this sample be evaluated?
What must be done if a sample will be read after 1 hour
refrigerate and bring to room temp prior to evaluation
The quality of the test results depend entirely on
quality of collection
Blood collection sites for bovine
jugular and coccygeal
Porcine blood collection sites
anterior vena cava and auricular vein (ear vein)
Ovine and caprine blood collection sites
jugular and cephalic
Avian blood collection sites
right jugular, ulnar (wing) vein, metatarsal vein
equine blood collection sites
jugular and transverse facial vein
Purple top tube contains what additive
EDTA
Red top tube contain what additive
none
Red and black (tiger top) tube and serum separator tube contains what anticoagulant
none
Purple top tube is used for
CBC all parts, azostix BUN
green top tube contains what additive
heparin
Green top tube is used for
blood gases, chemistry, reptile CBC
Blue top tube has what additive
sodium citrate
Blue top tube is for
coagulation studies
Grey top tube contains what additive
oxalate
Grey top tube is used for
blood glucose
Grey top tube w/o label=DET contains what additive
diatomaceous earth
Grey top tube w/o label=DET is used for
ACT (specific clotting test)
Red ring hematocrit tube is used for ____ and contains what additive
blood for CBC (except blood smear) directly from animal; heparin
Blue ring hematocrit tube is used for __ and contains what additive
transfer from EDTA for PCV, TP, blood smear; none
Black ring hematocrit tube is used for ___ and contains what additive
same ad red ring but uses less blood; heparin
A well-hydrated patient should yield a sample that is _fluid + _cells
50% + 50%
Order for sample collection
Citrate
RTT
Tiger top
GTT
LTT
What is the fluid portion of clotted blood
serum
What is the fluid portion of circulating or anti-coagulated blood
plasma
Why would protein levels be decreased in serum
clotting proteins are in the clot
Protein levels in plasma should be slightly _ than in serum
higher
Gases in blood
O2, CO2, Nitrogen
Proteins in blood
Albumin, Globulins, Clotting proteins like fibrinogen, Enzymes
Nutrients in blood
glucose, vitamins, lipids, amino acids
Electrolytes in blood
H+, Na+, K+, Cl-, Ca+2
Hormones in blood
T3, T4, Estrogen, etc.
Function of RBC
O2 transport
Function of WBC
defense
Function of platelets
clotting
Functions of fluid in blood
transports, perfusion, solvent, cellular chemical reactions
Fluid in blood is mainly
water
