Intro Flashcards
Lab test for spirochetes
Darkfield microscopy
Stain for legionella
Silver stain
Stain for chlamydia
Giemsa stain
Inclusion bodies
Stain for rickettsiae
Giemsa/tissue stains
Bacteria not seen in gram stain
These Rascals May Microscopically Lack Color Treponema Rickettsia Mycobacteria Mycoplasma Legionella Chlamydia
Its capsule is composed of polypeptide of D-glutamate
Bacillus anthracis
Obligate aerobes
Noisy and Nagging Pests Must Breath Lots of oxygen Nocardia Neisseria Pseudomonas Mycobacteria Bordetella/Brucella/B. cereus Legionella
Obligates Anaerobes
ABC of anaerobes
Actinomyces
Bacteroides
Clostridium
All bacteria have cell walls composed of peptidoglycan except?
Mycoplasma pneumoniae
All gram-positive bacteria have NO endotoxin except?
Listeria monocytogenes
All bacterial capsules are composed of polysaccharide except?
Bacillus anthracis
All exotoxins are heat-labile except?
Staphylococcal enterotoxin
Agar selective against gram-positive bacteria and differentiates between lactose fermenters and nonfermenters
Eosin-methylene blue (EMB)
MacConkey
Distinguishes lactose fermenters from nonfermenters and H2S producers from nonproducers
Triple sugar iron (TSI)
Egg yolk agar
Clostridium perfringens
Tellurite agar
C. diphtheriae
Bile esculin agar
Group D streptococci
Mannitol salts
Staphylococci (salt-loving)
Chocolate agar
N. meningitidis
N. gonorrhea from sterile sites (blood, csf)
Thayer-Martin agar
N. gonorrhea from nonsterile sites (oral, GUT)
Chocolate + factors X and V
H. influenzae
Lowenstein-Jensen/Middle brook agar
M. tuberculosis
Thiosulfate Citrate Bile Salts (TCBS)
V. cholerae
Bordet-gengou/Regan-Lowe agar
B. pertussis
Charcoal-yeast extract
Legionella pneumophila
Skirrows agar
Campylobacter
Helicobacter
Barbour-Stoenner-Kelly (BSK)
B. burgdorferi
Eaton agar
Mycoplasma pneumoniae
Cetrimide agar
Pseudomonas aeruginosa
Xylose-Lysine-Deoxycholate (XLD) agar
Salmonella
Shigella
EMJH/ Fletcher’s agar
Leptospira interrogans
For attachment and conjugation
Pilus or fimbria
Mediates adherence to surfaces
Glycocalyx
Genes for antibiotic resistance and toxins
Plasmid
Site of nutrients in cytoplasm
Granule
Bacterial growth curve (phase) with zero growth rate
Phase 1 Lag phase
Phase 3 Maximum stationary phase
Bacterial growth curve (phase) with negative growth rate
Phase 4 Decline or death phase
Type of DNA transfer from one bacterium to another
Conjugation
Type of DNA transfer by a virus from one cell to another
Transduction
Purified DNA is taken up by a cell
Transformation
Mediates attachment of bacteria
Pili
Bacteria with IgA protease
SHiNe My Gong S. pneumoniae H. influenzae N. meningitidis N. gonorrheae
Cell wall protein of S. aureus which prevents complement activation
Protein A
Cell wall protein of S. pyogenes which is antiphagocytic
M protein
Exotoxin subunit which is the active (toxic) subunit
A subunit B subunit (binding subunit)
B cell disorders (primary immunodeficiency disorders)
X-linked aggamaglobulinemia (Bruton’s)
Selective IgA deficiency
Common variable immunodeficiency
T cell disorders (primary immunodeficiency disorders)
Di George syndrome
Chronic mucocutaneous candidiasis
Combined B- and T- cell disorders
Severe combined immunodeficiency (SCID)
Wiskott-Aldrich Syndrome
Ataxia-Telangiectasia
Phagocyte disorders
Chronic granulomatous disease
Chediak-Higashi Syndrome
Leukocyte adhesion deficiency (LAD)
Complement disorders
Early (C2 or C3 deficiency)
Terminal
Recurrent infections with pyogenic bacteria indicate?
B-cell deficiency
Recurrent infections with fungi, viruses, or protozoa indicate?
T-cell deficiency
Virtual absence of B cells due to tyrosine kinase mutation; 6 mo old male
Bruton’s aggamaglobulinemia
Recurrent bacterial and sinus infections
Selective IgA deficiency
Defect in B cell maturation to plasma cells; mc form of severe antibody deficiency affecting both children and adults
Common variable immunodeficiency
Profound deficit in T cells; failure of dev’t of thymus and parathyroids due to a defect in 3rd and 4th pharyngeal pouches
Di George Syndrome
S/Sx of Di George Syndrome
Cardiac defect (TOF) Abnormal facies Thymic aplasia Cleft palate Hypocalcemia 22q11.2 chromosomal deletion
Specific T cell deficiency for C. albicans; recurrent candidiasis
Chronic mucocutaneous candidiasis
X-linked: defect in IL-2 receptors in T cells
Autosomal: ADA deficiency
Recurrent bacterial, viral, fungal, and protozoal infection in early infancy (3 months of age)
Bubble boy disease
Severe combined immunodeficiency
X-linked; inability to mount IgM response; mutation in wasp gene for actin filament assembly
Wiskott-Aldrich syndrome
S/Sx of Wiskott-Aldrich syndrome
Thrombocytopenia
Infections
Eczema
Wasp mutation
Ataxia, telangiectasia, recurrent infections by 2 years of age; IgA deficiency
Ataxia-Telangiectasia
Lack of NADPH activity; failure of oxidative burst; recurrent infections with catalase-positive bacteria and fungi (A. fumigatus)
Chronic granulomatous disease
Failure of phagolysosomal fusion; recurrent pyogenic infections cause by strep and staph
Chediak-Higashi syndrome
Mutation in integrins, defective adhesion (LFA-1) proteins on the surface of phagocytes; severe pyogenic infections in infancy; delayed separation of umbilical cord
Leukocyte adhesion deficiency (LAD)
MC complement defect
C2 deficiency
Specific deficiency in C5-C9; bacteremia with N. meningitidis or N. gonorrhea
Terminal complement deficiency
Stain for mycobacteria
Acid-fast stain
Loss of muscle control; shivering; myoclonic jerks and tremors; loss of coordination; rapidly progressive dementia; Dx? etiologic agent? Tx?
Progressive, neurodegenerative disease; Animal prion; no treatment available
Mad cow disease is caused by?
Prions
Sponge-like lesions in the brain; Dx? Etiologic agent?
Creutzfeldt-Jacob disease; Prions
Pulvinar sign on MRI; presence of florid plaques on neuropathology
Variant CJD
S/Sx of Classic CJD
Dementia
Early neurologic signs
S/Sx of Variant CJD
Prominent psychiatric and behavioral sx; painful dyesthesiasis; delayed neurologic signs
Obligate intracellular but acellular parasites of plants; naked RNA; no human diseases
Viroids
DNA pieces that move readily from one site to another either within or between the DNA of bacteria, plasmids, and bacteriophages; also called jumping genes
Transposons
DNa replication followed by insertion of new copy into another site
Replicative transposition
DNA is excised from the site without replicating and the inserted into the new site
Direct transposition
Gerstmann-Straussler-Scheinker syndrome (GSS syndrome) is caused by?
Prions
Completely dependent on oxygen for ATP generation
Obligate aerobes
Use fermentation but can tolerate low amounts of oxygen because they have superoxide dismutase
Microaerophiles
Utilize oxygen if it is present, but can use fermentation in its absence
Facultative anaerobes
Exclusively anaerobic but insensitive to the presence of oxygen
Aerotolerant anaerobes
Cannot grow in the presence of oxygen because they lack 3 important enzymes: superoxide dismutase, peroxidase, and catalase
Obligate anaerobes
Aerotolerant anaerobes
Propionibacterium
Lactobacillus
Facultative anaerobes
Staph Bacillus anthracis Corynebacterium Listeria Mycoplasma (except M. pneumoniae)
Microaerophiles
Strep
Spirochetes
Campylobacter
Helicobacter
Undergoes programmed rearrangement
Borrelia recurrentis
Toxins which undergo ADP ribosylation
Diphtheria
Cholera
E. coli
Pertussis