immunofluorescence Flashcards
why do we use fluorescence microscopy
to look at specific proteins in cells and tissues
how do we get a fluorescent protein
an antibody molecule or nucleotide probe will be coupled to a fluorescent dye
describe wavelength absorption and emission of a fluorescent dye
it absorbs light at one wavelength and emits it at a longer one
what is indirect immunofluorescence
a procedure where two antibodies are used, one anti-antigen and the other anti-primary
what type of cells produce antibodies
B cells
describe the structure of antibodies
Y shaped with two heavy chains and two light chains
what are the two regions of an antibody called
Fab and Fc
what is the Fab region of an antibody
the part that contains the light chain (+ the heavy chain portion it’s attached to)
what is the Fc region of an antibody
the part that only contains heavy chains
what part of antigens do antibodies recognize and bind to
epitope
what is an epitope
the part of an antigen that an antibody will bind to
define monoclonal antibody
a type of antibody that only binds to one epitope of an antigen
define polyclonal antibody
a type of antibody that can bind to different epitopes of an antigen
what type of fluorescent microscope are we using
EVOS FI
what ligthcube does the EVOS FI use
GFP lightcube
