experimental approaches

Question 1

what does hybridization refer to

Answer 1

the association via complementary base-pairing of two single stranded nucleic acids to form a double stranded molecule

Question 2

list 3 types of hybrids produced from hybridization

Answer 2

DNA-DNA, RNA-RNA, DNA-RNA

Question 3

what is southern blotting used to detect

Answer 3

fragments of a specific sequence

Question 4

list the steps of southern blotting

Answer 4

digest genomic DNA, run on gel to separate, denature the fragments via alkali solution, blot fragments onto membrane via capillary action, incubate with the probe = hybridization step, identify the fragments on the membrane

Question 5

list 3 ways that the fragments of southern blotting can be identified

Answer 5

autoradiography, chemiluminescnece, fluorescent imaging

Question 6

what does northern blotting detect

Answer 6

RNA

Question 7

where are riboswitches found

Answer 7

5’ UTR of mRNA

Question 8

not a flashcard, but go review the glycine riboswitch section for S pyogenes

Answer 8

you’re slaying :)

Question 9

what is western blot used to detect

Answer 9

protein

Question 10

list the steps of a western blot

Answer 10

isolate protein, separate using SDS-PAGE, transfer proteins from gel to membrane, detect protein of interest

Question 11

what does SDS-PAGE stand for

Answer 11

sodium dodecyl sulfide polyacrylamide gel electrophoresis

Question 12

list 3 modes of detection of the protein from a western blot

Answer 12

colorimetric, chemiluminescence, fluorescence