biosafety Flashcards
what does BSC stand for
biological safety cabinet
how many BSCs are there + what are they
3
class 1, 2, and 3
describe how BSC class 1 works
air flow is away from the operator, some air is filtered through a HEPA filter and released into the atmosphere
does class 1 protect the worker
yes, no air comes back out
does the class 1 provide a sterile working surface
no, some contaminated air is recircled through
what are the uses of class 1
routine operations that may generate harmful aerosols (used when product protection isn’t required)
how many types are in class 2 + what are they
4: A1, A2, B1, B2
describe how class 2 works
room air goes in and into the bottom where it is then funneled to the HEPA filter w/o interacting with the work surface. HEPA air blows outwards and onto the surface
does class 2 protect the worker
yes, no air comes back out
does class 2 provide a sterile work surface
yes, only sterile air interacts with the work surface
uses of class2?
cabinets are suitable for microbiology and tissue culture procedures
describe how class 3 works
cabinets are gas-tight and provide total containment.
does class 3 protect the worker
yes
does class 3 provide a sterile working surface
yes
describe how a laminar flow hood works
Room air goes in through the bottom (not over work surface), filtered air moves outwards over the surface
does laminar hood protect the worker
no, contaminated air flows back towards you
does laminar flow hood provide a sterile work surface
yes, only sterile air goes over the work surface
is the laminar flow hood a BSC
no
