biosafety Flashcards
what does BSC stand for
biological safety cabinet
how many BSCs are there + what are they
3
class 1, 2, and 3
describe how BSC class 1 works
air flow is away from the operator, some air is filtered through a HEPA filter and released into the atmosphere
does class 1 protect the worker
yes, no air comes back out
does the class 1 provide a sterile working surface
no, some contaminated air is recircled through
what are the uses of class 1
routine operations that may generate harmful aerosols (used when product protection isn’t required)
how many types are in class 2 + what are they
4: A1, A2, B1, B2
describe how class 2 works
room air goes in and into the bottom where it is then funneled to the HEPA filter w/o interacting with the work surface. HEPA air blows outwards and onto the surface
does class 2 protect the worker
yes, no air comes back out
does class 2 provide a sterile work surface
yes, only sterile air interacts with the work surface
uses of class2?
cabinets are suitable for microbiology and tissue culture procedures
describe how class 3 works
cabinets are gas-tight and provide total containment.
does class 3 protect the worker
yes
does class 3 provide a sterile working surface
yes
describe how a laminar flow hood works
Room air goes in through the bottom (not over work surface), filtered air moves outwards over the surface
