ICS - microbiolgy Flashcards

1
Q

define pathogen

A

Pathogen
Organism that causes or is capable of causing disease

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2
Q

Define Commensal

A

Commensal
Organism which colonises the host but causes no disease in normal circumstances

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3
Q

Define Opportunist Pathogen

A

Opportunist Pathogen
Microbe that only causes disease if host defences are compromised

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4
Q

Define Virulence/Pathogenicity

A

Virulence/Pathogenicity
The degree to which a given organism is pathogenic

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5
Q

Define Asymptomatic carriage

A

Asymptomatic carriage
When a pathogen is carried harmlessly at a tissue site where it causes no disease

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6
Q

what areas are open to bacterial colonistation

A

muscoal surfaces
The GI tract, lungs, bladder, kidnyes, urter, urethrer, gallbladder
out of thses, teh lungs and bladder should try and be bacterial free

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7
Q

what dhape is a cocus

A

spherical

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8
Q

what shap is a bacillus

A

rod

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9
Q

what colour is gram positive

A

purple

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10
Q

what color is gram negaitve

A

pink

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11
Q

what is a curved rod shaped bacteria

A

vibrio

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12
Q

what is teh name for spiral rod

A

spirochaete (sounds like a type of pasta)

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13
Q

what stain is for microbactrium

A

ziehl-neelsen stain (acid fast stain)

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14
Q

what are the differences between gram positive and gram negative bacteria

A

gram posiitve bacteria have a thick peptidoglygan layer and gram negative dont, they have a large lipoplysachoride (also known as endotoxin) layer instead

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15
Q

what are the two types of bacterial toxins

A

ENDOTOXIN
Component of the outer membrane of bacteria, eg lipopolysaccharide in Gram negative bacteria
EXOTOXIN
Secreted proteins of Gram positive and Gram negative bacteria

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16
Q

what is a toxoid

A

Toxoid is a toxin treated (usually with formaldehyde) so that it loses its toxicity but retains its antigenicity

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17
Q

what are teh features of an exotoxin

A

protien, specific action, strong antingencity, produced by gram + and - bacteria, and convertable into a toxoid

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18
Q

what are teh features of endotoxins

A

liposaccoride, non specific, produced only by gram negative, not convertable to a toxoid

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19
Q

in what 3 modes can bacteria gain new genetic information

A

conjugation - via a sex pilus
transformation - sent over in a plasmid
transduction - via phage (infected by a virus)

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20
Q

what are the three ways a bacterias DNAs can change

A

base substitution, deletion, insertation

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21
Q

what is obligate intracellular bacteria

A

bacteria that cannot be grown on an artifical media, only in human cells and tissues

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22
Q

what are the three tpes of obligate intracellular bacteria

A

rickettsia, chlamydia, coxiella

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23
Q

what are teh cypes of bacteria that can grwo on an artifical media and have no cell wall

A

mollicutes

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24
Q

what are the cocci, gram negative, anaerobic bacteria

A

veillonella

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25
what are teh cocci, which are gram negative and aerobic
neisseria and moraxella
26
what are the cocci, gram positive, aerobic, bacteria
staphylococcus, streptococcus
27
what test is done to distuinguish between streptococccus and what are the three outcomes
Macconkey beta heamolytic, alpha heamolytic, non heamolytic
28
what are the cocci, anerobic, gram posiitve bacteria
peptostrepococcus
29
how does staphylococcus often grow and how does streptococcus grow
in clusters, streptococcus grows in chains
30
what does coagulase mean
Coagulase: enzyme produced by bacteria that clots blood plasma.
31
what are teh three most important staphylococcus, and are therse coagulase positive or negative
s. aureus (because it looks gold) is coagulase positive s. epidermis is coagulase negative s. saphrophyticus - negative Staphylococcus' normal habitiat is the nose and skin
32
what is the information about Staphylococcus aureus
Spread by aerosol and touch carriers & shedders Virulence factors Pore-forming toxins (some strains) a - haemolysin Panton-Valentine Leucocidin ‘PVL’ Proteases Exfoliatin Toxic Shock Syndrome toxin (stimulates cytokine release) Protein A (surface protein which binds antibodies in wrong orientation) It causes wound infections on the skin (pyogenic) impertigo, pneumonia, sceptacemia it is also sotin mediated and can causes toxic shock and food poisnening
33
what is S.epidermidis
S.epidermidis Infections are ‘opportunistic’ immunocompromised, prostheses Main virulence factor - ability to form persistent biofilms
34
S.saprophyticus
S.saprophyticus Acute cystitis haemagglutinin for adhesion urease
35
what is heamolysis
destruction of red blood cells (in thsi case by bacteria to access teh nutrienst)
36
what test is done after teh alpha heamolytic and what are teh bacteria found?
optochin test resistant - viridans strep sentitive - s. pneumoniae
37
what are the beta and gamma heaolysis bacteria, and what lancefield group are they?
beta - complete lysis e.g. S.pyogenes (ABCG) gamma - no lysis S.Boris and Enterococcus (not a streptococcus) (D)
38
what is Antigenic sero-grouping and 2 exmaples
Antigenic sero-grouping (for Beta haemolytic strep only) It is done by using teh lancefiels micobead agglutination test, to put them into groups Group A - S.pyogenes thraot, skin, post partum Group B - S.agalactiae neonatal infections
39
what is teh Lancefield microbead agglutination test
Antiserum (antibodies) made that recognise each group Tagged to tiny plastic beads added to a suspension of bacteria Antibodies bind bacteria and beads clump together Visible to naked eye
40
what are the Infections caused by S.pyogenes
Respiratory Tonsillitis & pharyngitis Otitis media Skin and Soft tissue Wound infections Impetigo cellulitis puerperal fever Scarlet fever SPeA and M type Complications rheumatic fever glomerulonephritis
41
what are virulance factors
Virulence is described as an ability of an organism to infect the host and cause a disease. Virulence factors are the molecules that assist the bacterium colonize the host at the cellular level. These factors are either secretory, membrane associated or cytosolic in nature.`
42
what are the S.pneumoniae virulence factors
Capsule polysaccharide (84 types), antiphagocytic polysaccharide vaccine ‘PPV’ 23 types conjugate vaccine ‘PCV’ 13 types Inflammatory wall constituents teichoic acid (choline) peptidoglycan Cytotoxin pneumolysin
43
describe teh Viridans group streptococci
- haemolytic (or non-haemolytic) Optochin resistant Some cause dental caries & abscesses Important in infective endocarditits S. sanguinis, S. oralis Cause deep organ abscesses (e.g. brain, liver) Most virulent are the “milleri group” S.intermedius, S.anginosus, S.constellatus
44
what are the S.pyogenes virulence factors
Exported factors Enzymes Hyaluronidase - spreading Streptokinase - breaks down clots C5a peptidase - reduces chemotaxis Toxins Streptolysins O&S -binds cholesterol Erythrogenic toxin -Streptococcal pyrogenic toxin e.g. SPeA – exaggerated response Surface factors Capsule - hyaluronic acid M protein – surface protein (encourages complement degradation
45
what are three aerobic gram posiitve bascilli
Listeria monocytogenes Bacillus anthracis Corynebacterium diphtheriae
46
what are three anerobic gram posiitve bacteria
all from teh clostridia species C. tetani  - causes tetnus C. botulinum - causes botulism C. difficile - causes antibiotic assocaited diarrhea, pseudomembranous colitus
47
what makes a gram negative bacteria so special
it has two membranes, a phospholipd and a lipopolysacchoride
48
what are teh two types of virulence factors
coloinsation factors - adhesions, invasins, nuterin aquesition, defence against teh host toxins - secreted protiens that daeg
49
what does peritrichous flagella mean
the entire surface is coverd by flagella
50
what are proteobacteria
They come from the enterobacteria family they are rods are noramlly are motile with pertrichous flagella some specied colonise in teh intestinal tract
51
what is a MacConkey-lactose agar, how does it work, and what is an example of a lac positve and a lac negitive
this is a tye of agar that is pH senstiive and will turn red when the bacteria used lactose as theri food as it will make lactic acid, whihc has a low pH. Therefore if it is lac positive such as E Coli, it will be red and if it Lac negative, such as samonella or shigella, it will not show up red.
52
what is a serotype
it is a way of catogrinsing bacteria based on teh aino a cids and carbohydrates in teh cell surface. therre can be variation inbetween varietns of a single specied and different strains of teh same species Antigenically distinct variants of a single species are referred to as ‘serovars’, i.e. E. coli O157:H7 (an enterohaemorrhagic E. coli; EHEC) and E. coli O45:K1:H7 (a neonatal meningitis-associated E. coli, NMEC) are different serovars.
53
what are teh most commn infections cased by pathogenic Escherichia coli strains
Wound infections (surgical) (ii) UTIs (cystitis; 75-80% of ♀ UTIs - faecal source or sexual activity; catheterisation - most common type of nosocomial infection) (iii) Gastroenteritis (iv) Travellers’ diarrhoea (v) Bacteraemia (sometimes leading to sepsis syndrome) (vi) Meningitis (infants) - rare in UK
54
what is an example of how ecoli cause diohrea
teh toxin secreted causes teh pump in teh intestinal cells to be turned on and Cl- ions to be pumped out en mass thsi causes water to follow via osmosis whihc causes diorhea
55
what is dysantry
bloody dihoreah containg pus, blood and mucus
56
what is shigella
Very closely related to Escherichia (= “E. coli + virulence plasmid”) Four species: S. dysenteriae, S. flexneri, S. boydii, S. sonnei Shigellosis: severe bloody diarrhoea (bacillary dysentery) S. dysenteriae causes most severe form. S. sonnei most prevalent in developed world.
57
how does shigella invade the colonic mucosa
it is incredibly acid tolerant it enters though eh M cells - antigen sampling cells it then moves laterally though all of ghe gap junstions between cells and kills te enterocytes It also produces shiga toxin, which can traget teh kidneys and causes heamolutic uraemic kidneys this causes microvascular thromobosis in teh kidneys and causes kidney faliure
58
what are teh two types of samonella
S. enterica - responsible for salmonellosis >2,500 serovars* S. bongori - rare (contact with reptiles)
59
what are the three forms of salmonellosis caused by S. enterica:
1. Gastroenteritis/enterocolitis (serovars Enteritidis and Typhimurium) Frequent cause of food poisoning (milk, poultry meat & eggs) Second highest no. of food-related hospitalisations/deaths (UK) 6-36 hr incubation period, resolves (~7 days) Localised infection, only occasionally systemic 2. Enteric fever - typhoid/paratyphoid fever (serovars Typhi and Paratyphi) Poor quality drinking water/poor sanitation Systemic disease ~20 million cases, ~200,000 deaths/year (globally) 3. Bacteraemia (serovars Cholerasuis and Dublin) Uncommon
60
what is the process of pathogensis of salmonellosis
Ingestion of contaminated food/water - high I.D. (~106) (‘faecal-oral route’)  Invasion of gut epithelium (small intestine)  Transcytosed to basolateral membrane  Enters submucosal macrophages  Intracellular survival/replication
61
what is heamophilius influenza
a human parasite that is carried in the nasopharyngeal of many people,
62
what are 5 oportunistic infections
meningitus, bronchopneumonia, pneumonia in CF, COPD and HIV patients
63
what is blood agar and choclate gar
blood - agar jelly mixed with horse blood choclate agar - blood agar heated to lysis teh RBCs
64
what does the capsule of H.influenza do
penatrates the nasopharyngea epithelium resistance to phagocytosis and teh complenent system
65
what does fastidiuos bacteria mean
Microorganisms that are difficult to grow in the laboratory because they have complex or restricted nutritional and/or environmental requirements.
66
what bacteria is responibe for most of teh food poinsengiins in teh UK
camplobacter
67
what is mycobacteria and what is an example of it
the are a weakly gram positive but not classafied as it, acid fast staining bacteria. Thye have a thicker cell wall, with no capsule and dont form endospores. An example is Mycobacterium tuberculosis and mycobacterium leprae
68
what is teh structure of a mycobacteria
high lipid contnt with mycolic acids in there slightly curved bacilli rewact to teh zhiel neelson stain so are classified as acid fast they can survive in low pH enviroments and even inside of macrophages
69
how do macrophage fight off mycobacteria
phagocytosed by macrophage the bactuerim has evolved to escape into teh cytosol CD4T cells genreate interferon gamma to activate intracellular killing
70
how does a granulomma form
alveolar macrophages digest pathogens they then become more epithelioid and can fuse together to become langahan giant cells it get surronded by lymphocytes creasing a small pocked of ingfected macrophages fibroblasts are lad aroung it to wall it off teh central tissue necrosis If it works thenteh bacteria turn dorment, and if it doesnt there is just a cavidty of live bacteria that can causes condumption disease 9disseminated disease) later on
71
what are two reasons that granuomas can become unstable
CD4 depletion (can be caused by HIV) TNFa depletion by anti tumour necrosis factor drugs Ths causes teh bacteria to escape into teh body
72
what are teh two types of leprosy
tuberculoid leprosy - tissue hypersensitivity and granulomma, tissue damage to neerves lepromatous leprosy - lesions but poorly formed granulomas, skin lesion, TH2 based
73
how is TB diagnosed in clininc
The mantoux skin test The person is injected wth tuberculin and 2-3 days later comes back to see teh doctor if there is a reaised red bump they are TB posiitve - either latent or active
74
how is TB diagnosed in clininc
The mantoux skin test The person is injected wth tuberculin and 2-3 days later comes back to see teh doctor if there is a reaised red bump they are TB posiitve - either latent or active
75
what is a virus
an infections obligate intracellular parasite comprising of genetic materieral surronded by a proten coat and membrane
76
what are teh three shapes of a virus
helical isosahedreal complex
77
what 5 ways can virus3s cause disease
-direct destruction of host cell poliovirus - infects nerve cells and cuases lysis of teh cells cusing paralysis - modification of teh most cell rotavirus atrophies villi and causes flat epithelium cells whihc decreases surface area, causing sugar not to be absorbed and water to be draw out f teh cells cuaing dihorreha - over reactivity of teh immune system hepatitus B cuases teh cytotoxix T lymphocyte destory all infected cells causeing fanduc and destruction of hepatocytes - damage through cell proliferation HPV, cuases cervical cancer by putting its own DNA into host chromosomes causing oncoprotien to be made whihc causes cell mutations - Evasion of host defences leading to dormancy and later reinfection On a cellular level - Herpes virus goes latent in teh cells but never goes away, its teh same with chickenpoc and shingles later on On a moleculr level - the viruss mutates so its not recognized
78
what are the 5 types of vaccine makers and an example for each
inactivated - polio attenueated - MMR BCG secreted products - tetanus contituents of cell walls - Hep B recombiant components - experinmental
79
what is prophylacxis
prevenging teh disease from developing via using vaccine and so on
80
what are teh 5 major classificatoin of protozoa
Flaggelates - have a flagelum to propel them Amoebae - Sporozoan Cilliates - lots of little hairs to move them Microsporidia
81
what is sleeping skiness
sleeping skiness - caused by the tsetse fly bight whihc passes on teh protozoa causes a chancre, CNS changes and then coma and death, there are two types of this,
82
what is myalgia
pain in muslces
83
what is teh most fatal type of malaria
plasmodium falciparum
84
what are the syptoms of malaria
FEVER!!! chills, headache, mylagia, fatuigue, Diarrhoa, vomiting, abdominal pain
85
what are some signs of malaria
aneamia, jaundice, heaptoslpenomegally blac water fever (black urine)
86
what is teh malerial life cycle
three cycles exoerythrocytic cycle erethrocytic cycle sporogenuic cycle
87
what is teh sporogenuic cycle of maleria
how teh mosquito itself gets infected it feeds on blood from an infected human it creates an oocyte this bursts and frees lots of sporozoites
88
what is the exoerythrocytic cycle of maleria
teh parasite gets into teh hepatic cell reproduces by forming a schizont teh schizont bursts and spreads the protozoa THis cuses an inflamed liver and am=bdminal pain
89
what s the erethryocytic cycle of malaria
the parasite enters teh erethrocyte mature trophozoite turns into a schizont this ruptures and reeases more malaria into teh body thsi cuases aneamia, jaundice, haemoglobinuria
90
how does complicated malaria form and what areteh consequences
the infected red blood cells turn stick and adhere to eachother and the epithelium thsi causes mini blockedges in small blood vessles called microinfarcts cereberal - hypoglycemia causing coma and drowsyness renal - dehydration, hypotension, heamobloburina - fatuigue, things in urine ARDs - vascular occulsion, aneamia, acidosis, casuing hypoxia, pulmonary oedema
91
what are teh treatments for malaria
IV artesunate for complicated treat each organ affected - oxygen, fluids, antibiotics, blood products
92
defien host pathogen interactions
how pathogens sustain themseles withi teh host
93
defien host pathogen interactions
how pathogens sustain themseles withi teh host
94
what are the 4 stages of pathogenesis
exposure (contact), adhesion (colonization), invasion, infection.
95
what are commensal microorganisms
Commensal microorganisms are the resident flora and usually nonpathogenic
96
what is the humoral resoponse to viruses
antibodies - G, A, block binding, block virus host cell opsionation IgM - Agglutinates particals coplemetn factors - opsonation, lysis
97
what is teh cell mediated response to viruses
IFN from Th (CD4+ ) or Cytotoxic T lymphocytes (CTL)/Tc (CD8+) – has direct antiviral action CTL can kill infected cells NK cells and macrophages are involved in antibody-dependent cellular cytotoxicity (ADCC) killing IFN induces anti viral proteins for bystander cells
98
define antigenic drift and antigenic shift
Antigenic Drift - spontaneous mutations, occur gradually giving minor changes in HA (haemagglutinin) and NA (neuraminidase). Epidemics. Antigenic Shift - sudden emergence of new subtype different to that of preceding virus. Pandemics.
99
how is a gram stain completed
Fixation of clinical materials to microscope slide (heat/methanol) Application of primary stain: crystal violet (all cells turn purple) Application of mordant (iodine): crystal violet-iodine complex formed Decolourisation step: distinguishes gram +ve and gram -ve, use acetone or ethanol Application of counterstain: safranin to stain gram -ve pink the mnumonic for this is Come in and stain crystal violet, iodene, alcohol, safranin
100
what are the gram positive rods?
Gram +ve rods = Corneybacteria, Mycobacteria, Listeria, Bacillus, Nocardia
101
what is teh ost response to a bacterial infection
IgA(s) Block attachment to host cells Ab C3b Opsonisation, Prevents proliferation Complement Cell lysis, Prevents proliferation Ab Neutralise toxins
102
what are symptoms of meningitus
stiffness of the neck, photophobia + severe headache Infective: fever, malaise Petechial rash associated with meningococcal meningitis
103
what are teh tow most common bacterial causes of meningitus
Neisseria meningitidis, Streptococcus pneumoniae these are both diplococci
104
What would be present in the blood if meningitus was presant for bacteria, viral, TB
What would be present in the blood if SF sample (lumbar puncture at L4) for microscopy + sensitivity testing Bacteria: turbid yellow colour, neutrophil polymorphs, raised protein, low glucose Viral: lymphocytes, normal protein, normal glucose Tb: lymphocytes, raised protein, low/normal glucose Nose + throat swabs for viral
105
what are the treatment for viral and then bacterial meningitus
Bacterial: start antibiotics before tests come back if suspected Cephalosporins: IV cefotaxime/ IV ceftriaxone If over 50/immunocompromised add IV amoxicillin to cover listeria One dose oral ciprofloxacin - prophylaxis for contacts Meningococcal septicaemia: immediate IM benzylpenicillin in community/ IV cefotaxime in hospital Viral: supportive treatment, self-limiting in 4-10 days, acyclovir for HSV meningitis
106
what are teh catogries of antibiotics that inhibit cell wall synthesis
glycopeptides - vancomycin beta lactams - peniclins (flucoxacillin), cephalosporins (cephalein) carbapenems (imipenem)
107
what test is done after teh alpha heamolytic and what are teh bacteria found?
optochin test resistant - viridans strep sentitive - s. pneumoniae
108
what are the gram positive aerobic bacilli
bacillus anthratics corynebactera diptherria listeria monocytogenes
109
what are the gram negative positive mcConcey bacteria
E.coli Klebsiella
110
what are teh gram negative, negative McConkey, oxidase positive bacteria
pseudomonas campylobacter
111
what are the gram negative, McConkey positive, oxidase negative, bacteria
shigella salmonella protwas
112
what are teh three catogries of beta lactams and an an examle of each
penicillins - fluccloxicillin cephalosporins - cefotaxime, cefriaxone, cefuroxime carbapenems - meropenem
113
when would glycopeptide antibiotics be given
for gram positive only for penicilllin allergys if resistant to teh beta lactams
114
what are two glycopeptide antibiotics
vancomycin teicoplanin
115
what are teh 4 drug catogires of protien synthesis inhibitors and when is each one used
lincosamides - clindamycin (G+, cellulitus, nectosinf fasciitus) macolides - clarithromycin (penicillin allergys) tetracyclines - doxycycline (cellulitus, pneumonia) aminoglycosids - genatamicin (G- nd staphs, UTIs, Endocarditus)
116
what are teh folate synthesis blocker antibiotics
sulphonamides - trimothroprim and co-trioxazole
117
which antibiotic should never be giver to a pregnant woman
Folate synthesis blockers - no sulphonamides sulphemthoazole, trimethoprin
118
what is the broadest antobiotic and which catogry is it in
Meropenem carbopenem group
119
what are teh RNA synthesis blocker antibiotics
metronidazole ciprofloxacin
120
what are the folate blocker antibiotics and what are they used for
trimethoprim and co-trimxazole UTIs and mainly gor G-
121
what antibiotic would be given for staph infections
Flucloxacillin
122
what antibiotic would you give to treat a UTU
Trimethoprim
123
what antibiotic would you give for MRSA
Vancomyocin
124
what are two examples of mycobacteria diseases
TB and leprosy
125
what is myobacteria
Aerobic, non-spore forming, non-mobile bacilli Slow-growing causes gradual onset of disease Requires multi-antibiotic treatment for a prolonged period
126
what type of stain will show myobacteria
Ziehl- Neelson - its an acid fast
127
how do viruses cause disease
Can cause disease via direct destruction (polio), modification (rotavirus), over-reactivity (hepatitis B), damage through cell proliferation (HPV)
128
how are viral infections diagnosed
PCR + nucleic acid amplification tests (NAAT) used, can also do serology (look for antibodies in response to virus
129
what are teh antibodies for a viral infection
IgM within 1 week of onset, IgG appears later
130
what drug is teh main one given for viral infections
Acyclovir
131
what are protozoa
Microscopic unicellular eukaryotes
132
what are the 4 classification of protozoa
ameoboids, ciliates, sporozoan, flagellates
133
what are 4 examples of protozoa `
malaria, giardia lamblia, toxoplasmiosis, trichomonas vaginalis
134
what sjould a high fever and recent travel indicate
MALARIA!
135
what are fungi
Eukaryote Have a cell wall - chitin + glucans (polysaccharides) Move by growing across or through structures or by dispersion in air/ water
136
what are the 2 forms of fungi
Yeast - single cell that divide via budding Moulds - form multicellular hyphae or spores
137
what are some examples of diseases caused by fungi
candida, athlete’s foot, nappy rash
138
what do anti fungal drugs normally end in
-azole
139
what are the three classifications of worms
nematodes (roundworms), trematodes (flatworms), ceratodes (tapeworms
140
how do worms reproduce
Adult worms cannot replicate inside body without a period of development outside the body Pre-patent period = interval between infection + appearance of eggs/ larvae in stool
141
what are teh antibodies response to worm infections
Mainly IgG + IgE mediated
142
what are two exampes of worm caused diseases
hookworm, schistosomiasis
143
what are teh layers of teh HIV envelope
RNA + Capsid + RNA transcriptase
144
what are three diseases that heamophilus influenzea cause
meningitus in pre school children pharyngitus otisis media Exaubation of COPD
145
what are fungi
Eukaryotic Chitinous cell wall Heterotrophic “Move” by means of growth or through the generation of spores (conidia), which are carried through air or water
146
what is teh difference between yeast and mould
Yeasts are small single celled organisms that divide by budding Account for <1% of fungal species but include several highly medically relevant ones Moulds form multicellular hyphae and spores
147
what are some invasive fungi conditions taht can affect: imounocm[armised hosts, post surgical patients, and healthy hosts
Immunocompromised hosts: Candida line infections Invasive aspergillosis Pneumocystis Cryptococcosis Mucormycosis Post-surgical patients: Intra-abdominal infection Healthy hosts: Fungal asthma Travel associated fungal infections Dimorphic fungi Post-influenza aspergillosis
148
how are fungal infections diagnosed
radiology, microscopy, cultur, molecular (PCR and antigen)
149
why are fungo harder to treat than bacteria
they are eukaryotic, so more similar ot humans
150
what is one of teh best drug tyoes against fungi
attacking teh cell wall as humans dont have one or attacking teh cell membrane as teh humna one cntians cholesterol ad teh fungal one contains ergsterol
151
what is the broadests types of antifungal
amphotericin B
152
what is a bad CD4 level in HIV+ patients
anything below 200 if they have a normal Cd4 of above 500 it means that they can effectivly be treated as a normal patient
153
what are some fungal drug interactions
they react with cytochrome p450 enzymes and can inhibit them, thsi means that it shouldbe be taken with some drugs such as warfrin
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what was teh target for 2020 in teh world for HIV
90-90-90 90% diagnosed 90% treated 90% to an undetectable level
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what are teh three HIV transmission routes
Sexual Vertical (mother to child) Blood (needles - reduced due to teh needle exchange and also blood transfusoins screened)
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what is prep, what is PEP
pre-exposure prothylaxis take it before exposure to stop yourself from being able to get it PEP: Post exposure prophylaxis, you take it after you are exposed
157
what are the original symptoms of HIV
Generalised lymphadenopathy Acute generalised rash Glandular fever/ flu-like illnesses Think about seroconversion HIV rash it ofther on teh paksm of teh hands!
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what else can be confused with HIV wit teh first, flu and rash like sympotms
Syphyliss hand foot and mouth disease
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what are teh later sympotms of HIV
Unexplained weight loss or night sweats Persistent diarrhoea Gradually increasing shortness of breath and dry cough Recurrent bacterial infections including pneumococcal pneumonia
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how does HIV affect teh CD4 receptors
HIV fuses to teh receptors and passes on its content
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what are 4 drug targets for anti HIV
- entry point reverse transcriptase inhibitors Protease inhibitors - intergrase inhibitors
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how does teh immune system respond to HIV
One of the key immune responses to HIV-1, from CD4+ T-helper cells, is lost from very early in infection, because these are the cells HIV infects first There is a very vigorous response from cytotoxic CD8+ T-cells, which provides the major force controlling viral replication but ultimately fail when “immune exhaustion” sets in
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what makes HIV avoident of the immune system
it onlt contains a few envelops strikes tey antigens are highly glycosaeted whihc makes it haed for teh Antibodies to bind The envelope (gp120/41) proteins can change substantially without affecting virus function
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what is teh difference between gram pos and neg cell walls
pos - have a thick layer of peptidoglycan neg - have a thin latyer of peptidoglycan and an outer mebrane called lipopolysaccoride, which is also called endotoxin