human genomics Flashcards
genome
genetic information that is encoded into its DNA and can be inherited by its offspring
genome defined
code for proteins and other DNA sequences that do not code for a protein
DNA can be sequenced
to determine the order of bases along its molecule
sequence data requires
bioinformatics
systematics compares
human genome sequence data and the genomes of other species to provide information on evolution relationships and origins
personalised medicine
individual’s genome
analysis of an individual genome can lead to
personalised medicine
Polymerase chain of reaction (PCR) is a
laboratory technique for amplification of DNA
PCR uses primer completerary
to specific target sequence at the two ends of the DNA region to be amplified
In PCR heating separates the
two strands of DNA to be amplified
In PCR, the separated stands are
then cooled to allow primer to bind to target sequences
Heat-tolerant DNA polymerase
replicates the region of DNA that has been primed
repeated cycles of heating and cooling
amplify the region of DNA.
DNA probes are
short,single stranded fragments of DNA that are complementary to a specific DNA sequence.
In PCR, arrays of DNA probes are used
to detect the presence of specific sequence in strands of DNA
