Homogenisation Flashcards
1
Q
Sediments
A
1 - nuclei
2 - mitochondria, chloroplasts
3 - ribosomes
2
Q
Liquid part
A
Supernatant
3
Q
Stages + why
A
To separate bits of cells out for study
Homogenisation & ultracentrifugation
4
Q
Kept
A
Cold - so enzymes don’t digest the organelles
Buffered - pH constant so proteins don’t denature
Isotonic - same water potential so cells don’t lyse or shrink
