Homogenisation Flashcards

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1
Q

Sediments

A

1 - nuclei
2 - mitochondria, chloroplasts
3 - ribosomes

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2
Q

Liquid part

A

Supernatant

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3
Q

Stages + why

A

To separate bits of cells out for study

Homogenisation & ultracentrifugation

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4
Q

Kept

A

Cold - so enzymes don’t digest the organelles

Buffered - pH constant so proteins don’t denature

Isotonic - same water potential so cells don’t lyse or shrink

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