Homework Questions Flashcards
Which of the following statements about antisense RNA is true?
A. Antisense RNA binds to form double-stranded regions on RNA to either block translation or intron splicing.
B. Antisense RNA is transcribed using the sense strand of DNA as a template.
C. The sequence of antisense RNA is complementary to mRNA.
D. Antisense RNA is made naturally in cells and also artificially in the laboratory.
E. All of the above statements about antisense RNA are true.
E. All of the above statements about antisense RNA are true.
Which of the following terms describes when gene regulation occurs by short dsRNA molecules
triggering an enzymatic reaction that degrades the mRNA of a target gene?
A. post-transcriptional gene silencing
B. quelling
C. co-suppression
D. RNA interference
E. all of the above
E. all of the above
How can RNAi be triggered in mammalian cells?
A. transfection of siRNA
B. chemically synthesized siRNA
C. degradation of target mRNA through shRNA creation
D. modification of an existing shRNA to recognize a different mRNA
E. all of the above
E. all of the above
To construct an antisense gene:
A. The gene is cloned such that the sense (aka coding, nontemplate) strand of the gene is
transcribed.
B. The gene is cloned such that the antisense (aka noncoding, template) strand of the gene is
transcribed.
C. A terminator is placed on both sides of the gene.
D. The stop codon of the gene is removed.
A. The gene is cloned such that the sense (aka coding, nontemplate) strand of the gene is
transcribed.
A liposome antisense RNA delivery system is:
A. made of bilayers of phospholipids and cholesterol.
B. taken up by endocytosis
C. attached to peptides.
D. All of the above are true. (“can be” attached
E. A and B are true.
E. A and B are true.
The enzyme responsible for the production of siRNAs from dsRNA
A. Dicer
B. RISC
C. RNA-dependent RNA polymerase
D. streptolysin
A. Dicer
- These RNA molecules are transcribed from an endogenous gene in the organism as longer
precursor molecules before they are processed into small segments to trigger RNAi.
A. shRNA
B. siRNA
C. dsRNA
D. microRNA
C. dsRNA
Piwi-interacting RNAs function in .
A. defense against foreign RNA
B. X chromosome inactivation
C. transposon silencing in germ cell lines
D. gene regulation
E. RNA editing
C. transposon silencing in germ cell lines
CRISPR is
A. What you say at Popeyes or Bojangles when asked about your chicken order.
B. An acronym for ‘clustered regularly interspersed short palindromic repeat’.
C. The coupon code for your special BMME 485 student discount for your 23 and Me analysis.
D. None of the above.
E. All of the abov
D. None of the above.
What causes cancer?
A. somatic mutations that disrupt normal cell division and death
B. germline mutations that result in cell death
C. somatic mutations that cause cells to die prematurely
D. germline mutations that disrupt normal cell division and death
E. none of the above
A. somatic mutations that disrupt normal cell division and death
Which of the following is the main regulator for cellular reprograming to produce iPSCs?
A. OCT4
B. SOX2
C. NANOG
D. c-MYC
E. LIN28
A. OCT4
All of the following are totipotent except .
A. stem cells
B. mammary gland cells
C. cells of the blastula
D. cells of the inner cell mass
E. erythrocytes (red blood cells)
E. erythrocytes (red blood cells)
Stem cells that have lost the ability to divide are called ________.
A. totipotent
B. competent
C. undifferentiated
D. differentiated
E. transformed
D. differentiated
Spectrosomes .
A. are organelle-like complexes that are associated with mitotic spindle during Drosophila cell
division
B. contain spectrin and ankyrin
C. persist in the first cells after the stem cell divides
D. are markers used to identify stem cells
E. All of the above
E. All of the above
When one daughter cell receives a signaling molecule that the other daughter cells does not receive
is called .
A. extrinsic asymmetry
B. intrinsic asymmetry
C. symmetrical renewal
D. symmetrical differentiation
E. differentiation
B. intrinsic asymmetry
How are adult stem cells identified?
A. Lineage analysis of a marker within a labeled potential stem cell
B. Identification of organelle-like structures called spectrosomes
C. Genetic mosaic transplantation
D. Identification of signaling transduction molecules specific to stem cells.
E. All of the above
E. All of the above
Which of the following cells produces antimicrobial peptides and regulates the type of bacteria
found in the intestine?
A. Enterocytes
B. Paneth cells
C. Enteroendocrine cells
D. Goblet cells
E. Vascular cells
B. Paneth cells
CAR-T strands for
A. A singer
B. Cancer associated receptor therapy
C. Cancer associated receptor T-cell
D. Chimeric antigen receptor therapy
E. Chimeric antigen receptor T-cell
E. Chimeric antigen receptor T-cell
Which of the following is the most widely used stem cell for stem cell therapy?
A. HSC
B. ISC
C. iPSC
D. ESC
A. HSC
Name at least 3 different CAR constructs that are improvements from the original CAR T cell
construct (From Valenzuela’s lecture):
Dual CAR
Tandem CAR
Looped Tandem CAR
Which of the following are part of the human immune system (select all that apply)? (From
Valenzuela’s lecture)
A. Physical barriers (skin, hair, mucus)
B. Electrostatic repulsion
C. Chemical/cellular defenses
D. Adaptive immunity
E. Innate (natural) immunity
F. Hot steam
A. Physical barriers (skin, hair, mucus)
C. Chemical/cellular defenses
D. Adaptive immunity
E. Innate (natural) immunity
What process is used to create transgenic mice?
A. Particle bombardment
B. Nuclear microdissection
C. Nuclear fusion
D. Germ line transformation
E. None of the above
E. None of the above
According to the book, which protein is produced by transgenic goats?
A. Recombinant tissue plasminogen activator
B. Recombinant tissue fibrinogen activator
C. Recombinant bovine somatotropin
D. Recombinant human somatotropin
E. None of the above
A. Recombinant tissue plasminogen activator
Why are embryonic stem cells important?
A. They can be passed from one generation to the next.
B. These cells carry retroviral genes.
C. They can develop into any tissue in the body, including the germ line.
D. The cells are differentiated and can therefore be manipulated.
E. none of the above
C. They can develop into any tissue in the body, including the germ line.
