RNA and DNA Seq Technologies Flashcards

1
Q

454 Amplification?

A

Yes, Emulsion PCR

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2
Q

454 Sequencing by Synthesis?

A

Yes

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3
Q

454 Enzyme?

A

DNA polymerase

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4
Q

454 Detection?

A

Light from luciferase

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5
Q

454 Read Length?

A

Short

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6
Q

Ion torrent Amplification?

A

Yes, emulsion PCR

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7
Q

Ion Torrent Sequence By Synthesis?

A

Yes

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8
Q

Ion Torrent Enzyme?

A

DNA polymerase

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9
Q

Ion Torrent Detection?

A

H+ Semiconductor

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10
Q

Ion Torrent Read Length?

A

Short

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11
Q

Illumina Amplification?

A

Yes, Bridge

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12
Q

Illumina Sequence by Synthesis?

A

Yes

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13
Q

Illumina Enzyme?

A

DNA Polymerase

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14
Q

Illumina Detection?

A

Fluorescent

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15
Q

Illumina Read Length?

A

Short

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16
Q

SMRT Amplification?

A

No

17
Q

SMRT Sequence by Synthesis?

A

Yes

18
Q

SMRT Enzyme?

A

xDNA polymerase

19
Q

SMRT Detection?

A

Fluorescent Zerowave

20
Q

SMRT Read Length?

A

Long

21
Q

Oxford Nanopore Amplification?

A

No

22
Q

Oxford Nanopore Sequence by Synthesis?

A

No

23
Q

Oxford Nanopore Enzyme?

A

Helicase

24
Q

Oxford Nanopore Read Length?

A

Long