Exam 1- Lecture 10

Question 1

What is monoisotopic mass

Answer 1

Corresponds to the lowest mass peak of isotopic series

Question 2

What is SILAC used for

Answer 2

to compare relative amounts of one protein in two different experimental conditions

Question 3

How does SILAC work

Answer 3

two samples of cells are grown with and without amino acids tagged with a stable isotope
proteins isolated
individual peptides are two peaks: one normal and one with heavy sample

Question 4

What does SILAC analyze

Answer 4

ratio of two peaks will determine the relative experimental change in level of the protein of interest

Question 5

What does iTRAQ determine

Answer 5

determine the amount of proteins from different sources in a single experiment

Question 6

What happens during iTRAQ

Answer 6

uses heavy isotopes and isobaric tags to analyze different samples and protein expression

Question 7

What is phosphoproteomics

Answer 7

branch of proteomics that identifies, catalogs, and characterizes proteins containing a phosphate group as a posttranslational modification