HIV Flashcards

1
Q

HIV

A

Diploid: Carries two copies of (+) stranded RNA

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2
Q

RNA is tightly bound to

A

key enzymes

  • Reverse Transcriptase
  • Aspartate Protease
  • Integrase
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3
Q

Reverse Transcriptase

A

Makes RNA from DNA

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4
Q

Aspartate Protease

A

Cleavage of proteins

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5
Q

Integrase

A

Integrates DNA into host cell DNA

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6
Q

HIV shape

A

Conical capsid

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7
Q

HIV is composed of

A

multiple copies of nonvariable, p24 protein

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8
Q

Does immune system produce antibodies to HIV?

A

Yes, but they are not strong enough to neutralize virus

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9
Q

HIV envelope made of

A

phospholipids from human cell membrane

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10
Q

What protein does the envelop contain?

A

env

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11
Q

Cap of env protein contains

A

glycoproteins (gp120 and gp41)

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12
Q

gp120

A

attachment to T cells

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13
Q

gp41

A

fusion and entry into T-cells

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14
Q

gp120 and gp41 are both formed as a

A

single protein (gp160) and cleaved

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15
Q

Gene for gp 120 mutates

A

rapidly (antigenic variation)

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16
Q

V3 loop portion of gp120

A

highly immunogenic
so varies significantly
and antibody neutralization difficult

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17
Q

Antibodies develop against what portion of gp 120?

18
Q

3 main genes encode HIV virus

19
Q

Gag gene encodes

A

nucleocapsid (p24)

20
Q

Pol gene encodes

A

polymerase

  • reverse transcriptase
  • integrase
  • protease
21
Q

Env gene encodes

A

envelope proteins

-gp120: gp120+gp41

22
Q

Tat and Rev genes required for

A

viral replication

23
Q

tat

A

activates gene transcription

24
Q

rev

A

transports mRNA from nucleus to cytoplasm

25
Auxillary genes
Nef, viv, vpr, vpu, vpx | -Not required for replication (auxillary)
26
Nef
decrease CD4 proteins and MHC-I on T-Cell surfaces
27
Major lymphocyte receptors used by HIV to enter cells
CCR5 and CXCR4
28
CCR5-tropic viruses
Replicate in monocytes/macrophages (M-tropic) Can also infect dendritic cells (trafficking to lymph nodes) Occur early: sexually transmitted to macrophages
29
CXCR-tropic viruses
Replicate more efficiently in T-cells (T-tropic) | Occur later after infection has developed
30
Mutations to CCR5 gene associated with
decreased susceptibility to HIV infection
31
Which drug class does not work in HIV-2?
NNRTIs
32
Presentation of acute HIV syndrome often confused with
mononucleosis (EBV)
33
ELISA
Tests for antibody only. Sensitive but many false positives. Western blot used to confirm positive ELISA (specific).
34
4th generation tests
Combination tests for p24 antigen and HIV antibodies | If positive, antibody differentiation assay
35
Acute HIV
p24 and HIV antibodies are not generated yet but you can test for viral load (HIV RNA)
36
HIV PCR testing used for babies b/c
HIV antibodies from mother may exist for months
37
Pneumocystis Pneumonia
CD4<200 | TMP-SMX
38
Pneumocystis and Toxic Plasmosis
CD4<100 | TMP-SMX
39
Histoplasmosis (endemic areas)
CD4<100 | Itroconazole
40
Mycobacterium Avium Complex
CD4<50 | Azithromycin