HISTOPATHOLOGY Flashcards
- Is widely used in histopathology and diagnosis, as it allows for the identification of abnormalities in cell count and in enhancing the contrast of the structure under the
microscope.
A. Staining
B. Sectioning
C. Retaining
A. Staining
- Is a fixative used for examining embryo and brain tissue because of its superior
preservation of delicate nuclei and glycogen.
A. Bouin
B. Paraffin-formalin
C. Prussian blue
A. Bouin
- Colloquially referred to as the “Pap smear”, a cytological staining technique best
known for detecting cervical cancer in female patients.
A. Nissl Stain
B. Mucicarmine
C. Papanicolaou stain
C. Papanicolaou stain
- Tissue samples must undergo preparation through the following stages:
A. Fixation, processing, embedding, sectioning, and staining
B. Processing, fixation, embedding, sectioning, and staining
C. Processing, fixation, sectioning, embedding, and staining
A. Fixation, processing, embedding, sectioning, and staining
- Before staining a slide, the tissue has to be prepared and mounted onto a glass slide.
A. True
B. False
C. Maybe
A. True
- Mucin stains are best for the detection and dying of mucopolysaccharides. Examples of mucin stains include:
- Alcian blue
- Mucicarmine
- Congo red
- Period acidSchiff (PAS).
A. 1,2,3
B. 1,3,4
C. 1,2,4
C. 1,2,4
- A group of disease. That cause progressive weakness and loss of muscle is called
A. Anatomy
B. Atrophy
C. Hypertrophy
D. Dystrophy
D. Dystrophy
- A natural dye which is by far the most valuable staining reagent is
A. Orcein
B. Coal tar
C. Safranin
D. Hematoxylin
D. Hematoxylin
- Which is a disadvantage of frozen section
A. Much faster processing
B. Lack of morphological detail
C. No exposure to organic solvents
D. Minimal exposure to fixative
B. Lack of morphological detail
- Tissue that takes up stains are called
A. Basophilic
B. Acidophilic
C. Chromatic
D. Eosinophilic
C. Chromatic
- Which of the following organic solvents used in histopathology laboratory are NOT highly flammable
A. Chloroform
B. Ethyl alcohol
C. Methyl alcohol
D. Acetone
D. Acetone
- Which is BEST criterion for evaluating a safer alternative reagent to formalin?
A. Reagent cost
B. Ability to produce a morphological picture similar to living state of tissues
C. Degree of staining affinity
D. Extent of tissue shrinkage
B. Ability to produce a morphological picture similar to living state of tissues
- Which of the following statement is NOT true of automated tissue processing
A. Shortened processing sequence
B. Reduced processing time
C. Present intervals
D. Continuous agitation
C. Present intervals
- Before any staining can be done, the slides are by running them through xylene (or
substitutes) to alcohols to water
A. Deparaffinized
B. Hydrated
C. Dehydrated
D. Alcoholized
A. Deparaffinized
- The subjective evidence of disease is
A. Sign
B. Pain
C. Symptom
D. Tumor
D. Tumor
- Improper setting of the cover slip during mounting may be due to
A. Excessive mounting medium
B. Excess xylene
C. Excessive dehydrating agent
D. Inadequate mounting medium
C. Excessive dehydrating agent
- What is an in-vitro type of molecular testing
A. Polymerase chain reaction
B. HIV 1 antibody
C. Troponin
D. Point of care
A. Polymerase chain reaction
- is the stain used in the detection of Helicobacter pylori in gastric biopsy specimens.
(Also stain for mast cells)
A. Toluidine blue
B. Turnbull blue
C. Acridine orange
D. Sudan I|l
A. Toluidine blue
- A staining technique used to identity chromosomal material in specimen is
A. H&E
B. Feulgen
C. Gram
D. Papanicolau
B. Feulgen
- are cells that undergo frequent cell division (ex. Skin cells)
A. Labile cells
B. Stable cells
C. Permanent cells
D. None of the above
A. Labile cells
- Histologic examination shows koilocytosis, which results from infection with:
A. Adenovirus
B. Cytomegalovirus
C. Herpes simplex virus
D. Human papilloma virus
D. Human papilloma virus
- In an autopsy procedure, the prosecutor is the
A. Pathologist
B. Medical technologist
C. Assistant
D. Medical Laboratory Technician
A. Pathologist
- Instruments used in obtaining cervical samples for Pappanicolau smear, EXCEPT
A. Glass pipeke
B. Ayre’s spatula
C. Laryngeal cannula
D. Syringe
D. Syringe
- It is a type of biopsy in which small pieces of tumor are removed with special
forceps
A. Punch biopsy
B. Bite biopsy
C. Wedge biopsy
D. Incisional biopsy
B. Bite biopsy
- The technique of autopsy is the widely used method
A. Technique of R. Virchow
B. Technique of c. Rokitansky
C. Technique of A. Ghon
D. Technique of M. Letulle
A. Technique of R. Virchow
- The time between specimen receipt and issuing of the pathologist report is the time
A. Log
B. Lag
C. Processing
D. Turnaround
D. Turnaround
- The accreditation of a water analysis laboratory shall expire
A. Anniversary date
B. Last day of December of the year started
C. One year from date of issue
D. 90 days before end of the year
C. One year from date of issue
- In the efficient administration of a clinical laboratory, the following are considered inputs, EXCEPT
A. Equipment
B. Supplies
C. Operating budget
D. Satisfactory performance
D. Satisfactory performance
- Immediate fixation of smears for cytology preserves fine structure of the chromatin
and helps in the evaluation of nuclear changes. This is achieved through the use of
A. Buffered formalin
B. 95% ethanol
C. 10% methanol
D. NSS
B. 95% ethanol
- The endocrine system is composed of the following, except
A. Ovary
B. Thyroid
C. Pancreas
D. Skin
D. Skin
- Which of the following is considered an acid dye
A. Eosin
B. Thionine
C. Azure ||
D. Toluodine Blue
A. Eosin
- The following are the types of specimens received in a histopathology laboratory,
except
A. Sputum smears
B. Excisions biopsies
C. Curretings
D. Core biopsies
A. Sputum smears
- The following characterizes honing, except
A. Heel to toe
B. Removal of nicks
C. Knife sharpening
D. Uses paddle made of horse leather
D. Uses paddle made of horse leather
- Aminoethylcarbazole (AEC), which is in color, is a common chromogen from peroxidase which should be made fresh immediately before use
A. Red
B. Brown
C. Orange
D. Pink
A. Red
- Which of the following term is used to identify a chemical that causes birth defects
A. Mutagen
B. Teratogen
C. Carcinogen
D. Reactive
B. Teratogen
- He invented the Cambridge microtome
A. Minot
B. Adams
C. Treefall
D. Queckek
C. Treefall
- Polyclonal antibodies used in immunohistochemical techniques are derived from
A. Rabbit
B. Goat
C. Pig
D. Mice
A. Rabbit
- He invented the Cambridge microtome
A. Minot
B. Adams
C. Treefall
D. Queckek
C. Treefall
- This used to remove the ethanol
A. Paraffin wax
B. Xylene
C. Eosin
B. Xylene
- Also called the Cresyl Violet Stain, it uses basic aniline dye to study neuronal
structure in the brain and spinal cord
A. Silver Stain
B. Masson’s Trichrome
C. Nissl Stain
C. Nissl Stain
- The most common dye used on hydrophobic fat or lipids, substances that are
traditionally difficult to stain
A. Congo Red
B. Oil Red O
C. Giemsa Stain
B. Oil Red O
- This step is to be performed with caution if the goal is to perform immunostaining
because the paraffin wax will inhibit the penetration of antibodies, and lead to a false
result
A. Staining
B. Fixation
C. Embedding
C. Embedding
- Haemotoxylin stains certain parts of the cell - like the cytoplasm - blue; Eosin stains other parts of the cell - such as the nucleus - red or pink
A. True
B. False
C. Maybe
A. True
- Trichrome stains, as the name indicates, are mixture of three dyes used to differentiate the muscles, collagen fibers, fibrin and erythrocytes in connective tissue
A. True
B. False
C. Maybe
A. True
- Select an example of physiologic hyperplasia
A. Formation of warts
B. Chancre
C. Pregnant uterus
D. Benign tumors
C. Pregnant uterus
- Following dehydration, the tissue sections are immersed in different Immersions
A. Methanol
B. Toluene
C. Xylene
D. Ethanol
D. Ethanol
- The section/ unit of the laboratory that tests for alcohol, heavy metals and poison is
A. Serology
B. Cytology
C. Toxicology
D. Endocrinology
C. Toxicology
- The hardening of muscle cells that begins shortly after death and causes board-like
stiffness in about 12 hours is
A. Livor mortis
B. Algor mortis
C. Rigor mortis
D. Post mortem lividity
C. Rigor mortis
- The commonly used dealcoholizing agent is
A. Peanut oil
B. Xylene
C. Absolute alcohol
D. Acetone
B. Xylene
- What is a significant variable to be considered when processing tissue
A. Manner of processing
B. Type of embedding medium
C. Type of microtome
D. Temperature
A. Manner of processing
