Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

MLSP112 > HISTOPATHOLOGY > Flashcards

HISTOPATHOLOGY Flashcards

1
Q

● Involves different procedures that have been adopted for the preparation of materials and tissue for microscopic
examination

A

HISTOPATHOLOGIC TECHNIQUES

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

12 STEPS IN HISTOPATHOLOGIC
TECHNIQUES:

A
  1. Numbering
  2. Fixation
  3. Dehydration
  4. Clearing
  5. Wax impregnation
  6. Embedding
  7. Blocking
  8. Trimming
  9. Sectioning
    10.Staining
    11.Mounting
    12.Labeling
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

● Validates if the specimen is adequate or good for tissue processing
● 1st person that will receive the specimen and put it into the container

A

RECEPTIONIST

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Container: contain fixatives

A

○ Proportional to the size of the specimen
○ Clear
○ Unbreakable
○ Wide mouthed bottle

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Specimen source

A

○ Bilateral organs
○ Miscellaneous

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Bilateral organs

A

Extremities, kidneys, lungs and ovaries

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Miscellaneous

A

Age, sex, ward

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Basic information needed:

A

○ Date and time
○ Name of the patient
○ Specimen number

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

CAS

A

■ C - Cytology
specimen
■ A - Anatomical
specimen
■ S - Surgical
specimen
● S-09-2111

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

● Most critical step in histopathological techniques

A

FIXATION

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

preserve the morphology and chemical constituents of the tissue.

A

Primary aim

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

protect and harden the specimen for
further handling

A

Secondary aim

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Effects Of Fixatives

A
  1. Inhibit bacterial growth and reduce the risk of infections
  2. Act as mordant or accentuator accelerating the staining process
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Involves small tissue or organ

A

MICROANATOMIC FIXATIVE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

● 10% Formol Saline
● 10% Neutral
Buffered
Formalin

A

MICROANATOMIC FIXATIVE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Involves body fluid or secretion
1. Nuclear Fixatives
2. Cytoplasmic Fixatives

A

CYTOLOGICAL FIXATIVE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Flemming’s Fluid, Bouin’s Fluid, Heidenhain’s Susa

A

Nuclear Fixatives

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

– Kelly’s Fluid, Orth’s

A

Cytoplasmic Fixatives

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Involves tissue containing labile
substances

A

HISTOCHEMICAL FIXATIVE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q
  1. 10% Formol Saline
  2. Absolute Ethyl Alcohol
  3. Acetone
A

HISTOCHEMICAL FIXATIVE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

SIMPLE FIXATIVE

A

Uses only on chemical for fixation
1. Aldehydes
2. Metallic Fixatives

22
Q

Formaldehyde, Glutaraldehyde

A

Aldehydes

23
Q

Mercuric Chloride, Chromate Fixatives, Lead Fixatives

A

Metallic Fixatives

24
Q

The use of two or more chemicals for fixation

A

COMPOUND FIXATIVE

25
Q

● Utilizing chemical known as dehydrating agents
● Removing of intracellular and extracellular water and fixatives in the tissue

A

DEHYDRATION

26
Q

DEHYDRATION Examples:

A

○ Alcohol - most commonly used
○ Acetone
○ Dioxane
○ Tetrahydrofuran
○ Cellosolve (Ethylene glycol monoethyl ether)

27
Q

● Removing of dehydrating agents
● Xylene – most commonly used

A

CLEARING

28
Q

● Also known as INFILTRATION
● The process whereby the clearing agent is completely removed from the tissue and replace by a medium that will completely fill all the tissue
cavities.

A

IMPREGNATION

29
Q

The simplest, most common and best
embedding medium used for routine
tissue processing

A

PARAFFIN

30
Q

● Also known as CASTING OR BLOCKING
● The process by which the impregnated tissue is placed into a precisely arrange position in a mold containing medium which is then allowed to solidify

A

EMBEDDING

31
Q

FOUR TYPES OF TISSUE IMPREGNATION:

A
  1. Paraffin wax
  2. Celloidin
  3. Gelatin
  4. Plastic
32
Q

● Allows the medium to solidify to produce tissue block

A

BLOCKING

33
Q

● Process of removing excess wax after embedding
● Can use knife/blade or heated spatula

A

TRIMMING

34
Q

● Also known as CUTTING OR MICROTOMY
● The process by which processed tissue is cut into uniformly thin slices to facilitate studies under microscope

A

SECTIONING

35
Q

machine or instrument used for cutting sections of tissue

A

MICROTOME

36
Q

Simplest and oldest type of microtome

A

ROCKING MICROTOME

37
Q

Used for cutting serial sections of tissue specimen

A

ROTARY MICROTOME

38
Q

Most dangerous type of microtome

A

SLIDING MICROTOME

39
Q

For urgent surgical biopsies specimen

A

FREEZING MICROTOME

40
Q

Permits rapid penetration of tissue biopsies for surgical pathology Also known as COLD MICROTOME

A

ROTARY MICROTOME

41
Q

Specimen for electron microscope

A

ULTRATHIN MICROTOME

42
Q

● Tissue constituent are demonstrated in sections by direct interaction with dye or staining solution producing
coloration of the active tissue component

A

STAINING

43
Q

○ Utilizes micro-anatomical studies of tissue
○ It is a regressive staining method

A

HEMATOXYLIN AND EOSIN STAINING

44
Q

● It may be liquid, gum or resinous, soluble in water, alcohol or other solvents and be sealed from the external atmosphere by non-soluble
ringing media

A

MOUNTING

45
Q

the solution in which the specimen is
embedded, generally under a cover glass

A

MOUNTING MEDIUM

46
Q

LABELING

A

● Date and time
● Name of the patient
● Specimen Number

47
Q

● Performed regularly even in pregnant women without undue risk

A

GYNECOLOGICAL SPECIMEN

48
Q

GYNECOLOGICAL SPECIMEN
EXAMPLE:

A

○ Vaginal smear

49
Q

NON-GYNECOLOGICAL SPECIMEN
EXAMPLE:

A

○ Respiratory Tract specimens:
■ Sputum
■ Bronchoalveolar lavage (BAL)

50
Q

Determine the presence of urethral cancer

A

URINE

MLSP112 (11 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions