Histopathology Flashcards
Who is the father of histopathology?
Johannes Muller
Who is the founder of pathology that used different methods of fixation?
Marcello Malphigi
Who discovered formalin?
Professor F. Blum
What do you call the specimen source when the specimen was taken from the morgue?
Autopsy (from dead)
What do you call the specimen source when the specimen was taken from the operating room?
Biopsy (from alive)
Identify the basic technique when preparing for routine histopathologic examination.
- the process of recording the tissue specimen in a logbook and assigning identification numbers to the specimen
Number
Identify the basic technique when preparing for routine histopathologic examination.
- preserving the tissue specimen in a life-like manner as possible
Fixation
Identify the basic technique when preparing for routine histopathologic examination.
- the process of removing water from the specimen by using grades of ethyl alcohol
Dehydration
Identify the basic technique when preparing for routine histopathologic examination.
- known as de-alcoholization
- the process of removing excess alcohol in the tissues and making the tissues transparent
Clearing
What is the routine clearing agent when doing a clearing technique for routine histopathologic examination?
Xylene
Identify the basic technique when preparing for routine histopathologic examination.
- the process of filling up tissues spaces or cavities using melted paraffin wax.
Infiltration
Identify the basic technique when preparing for routine histopathologic examination.
- the process of placing the infiltrated tissue inside a mold
Embedding or Molding
Identify the basic technique when preparing for routine histopathologic examination.
- the process of removing the excess paraffin wax from the block until it assumes
the shape of a truncated pyramid
Trimming
Identify the basic technique when preparing for routine histopathologic examination.
- also known as microtomy
- a tissue block is cut into thin slices called ribbons/sections using a microtome
Sectioning
Identify the basic technique when preparing for routine histopathologic examination.
- the process that employs the use of hematoxylin and eosin dyes to differentiate the cells and the cellular components
Staining