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HISTOPATH- Lec- Special Stains Flashcards

1
Q
  1. Mallory’s Phosphotungstic Acid Hematoxylin
  2. Highman’s Congo Red
  3. Alkaline Congo Red
  4. Kraijan’s Amyloid Stain
  5. Methyl Violet-Crystal Violet Method
  6. Induced Flourescent Staining with Thiotlavine-T

The ff are staining for?

A

CONNECTIVE TISSUES STAINS

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2
Q

Mallory’s Phosphotungstic Acid Hematoxylin
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Amyloid

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3
Q

Highman’s Congo Red
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Amyloid

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4
Q

Alkaline Congo Red
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Amyloid

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5
Q

Kraijan’s Amyloid Stain
stain specifically for?

A

Amyloid

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6
Q

Methyl Violet-Crystal Violet Method
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Amyloid

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7
Q

Induced Flourescent Staining with
Thiotlavine-T
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Amyloid

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8
Q
  1. Orcein (Taenzer-Unna)
  2. Weigert’s Elastic Tissue Stain
    The ff are staining for?
A

Staining of CONNECTIVE TISSUES STAINS

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9
Q

Orcein (Taenzer-Unna)
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Elastic Fibers

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10
Q

Weigert’s Elastic Tissue Stain
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Elastic Fiber

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11
Q

Kraijan’s Technique
staining of?

A

CONNECTIVE TISSUES STAINS

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12
Q

Kraijan’s Technique
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Elastic Fiber, Fibrin, Amyloid

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13
Q

Van Gieson (Acid-Fuchsin-Picric Acid)
staining for?

A

CONNECTIVE TISSUES STAINS

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14
Q

Van Gieson (Acid-Fuchsin-Picric Acid)
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Collagen

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15
Q

Masson’s Trichrome stain
staining for?

A

CONNECTIVE TISSUES STAINS

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16
Q

Masson’s Trichrome stain
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Collagen Fibers

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17
Q

Gomori’s Silver Impregnation
staining for?

A

CONNECTIVE TISSUE STAIN

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18
Q

Gomori’s Silver Impregnation
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Reticulin Fibers

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19
Q

Best known as an indicator

A

Congo Red

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20
Q

Congo Red
staining for?

A

CONNECTIVE TISSUE STAIN

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21
Q

Congo Red
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Elastic tissues, amyloid, myelin

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22
Q

Crystal Violet
staining for?

A

CONNECTIVE TISSUE STAIN

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23
Q

Crystal Violet
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Amyloid (frozen section)
Platelets in blood

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24
Q

Borohyde Periodic Shiff
staining for?

A

Staining of FATS and LIPIDS STAINS

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25
Borohyde Periodic Shiff Staining of FATS and LIPIDS STAINS stain specifically for?
Gangliosides
26
Nike Blue Sulfate staining of?
Staining of FATS and LIPIDS STAINS
27
Nike Blue Sulfate Staining of FATS and LIPIDS STAINS stain specifically for?
Neutral Fats Cholestrin Esters and Fatty Acids Cerebrosides
28
Oil Red O Method Dextrin staining for?
Staining of FATS and LIPIDS STAINS
29
Oil Red O Method Dextrin Staining of FATS and LIPIDS STAINS stain specifically for?
Fats
30
Osmic Acid Stain staining for?
Staining of FATS and LIPIDS STAINS
31
Osmic Acid Stain Staining of FATS and LIPIDS STAINS stain specifically for?
Unsaturated fats
32
Sudan IV (Scharlach R) staining for?
Staining of FATS and LIPIDS STAINS
33
Sudan IV (Scharlach R) Staining of FATS and LIPIDS STAINS stain specifically for?
Triglycerides
34
Toluidine Blue Acetone Method staining for?
Staining of FATS and LIPIDS STAINS
35
Toluidine Blue Acetone Method Staining of FATS and LIPIDS STAINS stain specifically for?
Sulfatide Deposits
36
Acridine Orange Flourescent (Masson Stain) staining for?
Staining of NUCLEIC ACID
37
Acridine Orange Flourescent (Masson Stain) Staining of NUCLEIC ACID stain specifically for?
RNA and DNA
38
Methyl Green-Pyronin stain for?
Staining of NUCLEIC ACID
39
Methyl Green-Pyronin Staining of NUCLEIC ACID stain specifically for?
RNA and DNA
40
Most reliable and specific histochemical staining technique for DNA
Fuelgen's Technique
41
Fuelgen's Technique staining for?
Staining of NUCLEIC ACID
42
Fuelgen's Technique Staining of NUCLEIC ACID stain specifically for?
DNA
43
Heidenhain's Iron Hematoxylin staining for?
Staining of MUSCLE and BONE STAIN
44
Heidenhain's Iron Hematoxylin Staining of MUSCLE and BONE STAIN stain specifically for?
Muscle striations Mitochondria Myelin Chromatin
45
Lissamine Fast-Red stain for?
Staining of MUSCLE and BONE STAIN
46
Lissamine Fast-Red Staining of MUSCLE and BONE STAIN stain specifically for?
Muscle and Bones
47
Tartrazine Method stain for?
Staining of MUSCLE and BONE STAIN
48
Tartrazine Method Staining of MUSCLE and BONE STAIN stain specifically for?
Muscle and Bones
49
Mallory's Phosphotungstic Acid Hematoxylin stain for?
Staining of MUSCLE and BONE STAIN
50
Mallory's Phosphotungstic Acid Hematoxylin Staining of MUSCLE and BONE STAIN stain specifically for?
Muscle Neuroglia Myelin Collagen
51
Modified Gomori's Trichrome stain for?
Staining of MUSCLE and BONE STAIN
52
Modified Gomori's Trichrome Staining of MUSCLE and BONE STAIN stain specifically for?
Muscle Fibers and Collagen
53
Schnorl's Picro Thionine Method stain for?
Staining of MUSCLE and BONE STAIN
54
Schnorl's Picro Thionine Method Staining of MUSCLE and BONE STAIN stain specifically for?
Bones (lacunae, canaliculi and bone matrix)
55
Rapid Toluidine-Eosin Stain stain for?
Staining of BONE MARROW and BLOOD ELEMENTS
56
Rapid Toluidine-Eosin Stain Staining of BONE MARROW and BLOOD ELEMENTS stain specifically for?
Glycol Methcrylate section
57
Peroxidase reaction stain for?
Staining of BONE MARROW and BLOOD ELEMENTS
58
Peroxidase reaction Staining of BONE MARROW and BLOOD ELEMENTS specifically for?
Myeloid cells
59
Wright's Giemsa Jenner staining for?
Staining of BONE MARROW and BLOOD ELEMENTS
60
Wright's Giemsa Jenner Staining of BONE MARROW and BLOOD ELEMENTS specifically for?
Cytoplasmic granules
61
Neutral Red stain for?
Staining of BONE MARROW and BLOOD ELEMENTS
62
Neutral Red Staining of BONE MARROW and BLOOD ELEMENTS specifically for?
Cell granules Vacuoles of phagocytic cells
63
Alpha Naphthyl Acetate stain for?
Staining of ENZYMES
64
Alpha Naphthyl Acetate Staining of ENZYMES specifically for?
Non-specific esterases
65
Gomori Calcium Method stain for?
Staining of ENZYMES
66
Gomori Calcium Method Staining of ENZYMES specifically for?
Alkaline Phosphatase
67
Gomori Dead Method stain for?
Staining of ENZYMES
68
Gomori Dead Method Staining of ENZYMES specifically for?
Acid Phosphatase
69
Lead Method (Wachstein and Meisel) stain for?
Staining of ENZYMES
70
Lead Method (Wachstein and Meisel) Staining of ENZYMES specifically for?
5’-nucleotidase
71
Indoxyl Acetate Method (Holt and Withers) stain for?
Staining of ENZYMES
72
Indoxyl Acetate Method (Holt and Withers) Staining of ENZYMES specifically for?
Esterases Activity
73
Tetrazolium Method stain for?
Staining of ENZYMES
74
Tetrazolium Method Staining of ENZYMES specifically for?
Monoamine oxidase activity
75
Alcian Blue Technique stain for?
Staining of CARBOHYDRATES STAIN
76
Alcian Blue Technique Staining of CARBOHYDRATES STAIN specifically for?
Most popular method for demonstration of ACID MUCINS
77
Combined Alcian Blue stain for?
Staining of CARBOHYDRATES STAIN
78
Combined Alcian Blue Staining of CARBOHYDRATES STAIN specifically for?
Demonstration of Mucins Separating acid mucin and neutral mucin
79
Best Carmine stain for?
Staining of CARBOHYDRATES STAIN
80
Best Carmine Staining of CARBOHYDRATES STAIN specifically for?
Fibrin Glycogen Mast cell granules Mucin
81
Flourescent Acridine Orange stain for?
Staining of CARBOHYDRATES STAIN
82
Flourescent Acridine Orange Staining of CARBOHYDRATES STAIN specifically for?
Acid mucopolysaccaride
83
Flourescent Acridine Orange -Can only last for _____ once the section is mounted
2 hours
84
Fresh frozen Azure-A stain for?
Staining of CARBOHYDRATES STAIN
85
Fresh frozen Azure-A Staining of CARBOHYDRATES STAIN specifically for?
Glycosaminoglycans
86
Hale's Dialyzed Iron Technique (colloidal) stain for?
Staining of CARBOHYDRATES STAIN
87
Hale's Dialyzed Iron Technique (colloidal) Staining of CARBOHYDRATES STAIN specifically for?
Acid Mucins
88
Langhan's lodine Method or Carleton's Method stain for?
Staining of CARBOHYDRATES STAIN
89
Langhan's lodine Method or Carleton's Method Staining of CARBOHYDRATES STAIN specifically for?
Not specific for Glycogen May also stain Amyloid
90
Metachromatic Toluidine Blue stain for?
Staining of CARBOHYDRATES STAIN
91
Metachromatic Toluidine Blue Staining of CARBOHYDRATES STAIN specifically for?
Glycosaminoglycans
92
Mucicarmine stain stain for?
Staining of CARBOHYDRATES STAIN
93
Mucicarmine stain Staining of CARBOHYDRATES STAIN specifically for?
Mucins
94
Periodic Acid Shiff/ PAS stain for?
Staining of CARBOHYDRATES STAIN
95
Periodic Acid Shiff/ PAS Staining of CARBOHYDRATES STAIN specifically for?
Glycogen Glomerular basement membrane (+) Magenta
96
PAS with Diastase stain for?
Staining of CARBOHYDRATES STAIN
97
PAS with Diastase Staining of CARBOHYDRATES STAIN specifically for?
Glycogen
98
Southgate's Mucicarmine Technique stain for?
Staining of CARBOHYDRATES STAIN
99
Southgate's Mucicarmine Technique Staining of CARBOHYDRATES STAIN specifically for?
Encapsulated fungi like cryptococcus neoformans
100
Toluidine Blue staining for?
Staining of MICROORGANISMS STAIN
101
Toluidine Blue Staining of MICROORGANISMS STAIN specifically for?
Helicobacter
102
Cresyl Violet Acetate Method stain for?
Staining of MICROORGANISMS STAIN
103
Cresyl Violet Acetate Method Staining of MICROORGANISMS STAIN specifically for?
Helicobacter
104
Levaditi's Stain stain for?
Staining of MICROORGANISMS STAIN
105
Levaditi's Stain Staining of MICROORGANISMS STAIN specifically for?
Spirochetes
106
Auramine-Rhodamine Flourescent Method
Staining of MICROORGANISMS STAIN
107
Auramine-Rhodamine Flourescent Method Staining of MICROORGANISMS STAIN specifically for?
Mycobacteria
108
Warthin-Starry Method stain for?
Staining of MICROORGANISMS STAIN
109
Warthin-Starry Method Staining of MICROORGANISMS STAIN specifically for?
Spirochetes
110
Brown and Brenn stain for?
Staining of MICROORGANISMS STAIN
111
Brown and Brenn Staining of MICROORGANISMS STAIN Specifically for?
Bacteria
112
Dieterle Stain stain for?
Staining of MICROORGANISMS STAIN
113
Dieterle Stain Staining of MICROORGANISMS STAIN specifically for?
Legionella pheumophilla
114
Grocott Methamine Silver stain for? stain specifically for?
Staining of MICROORGANISMS STAIN Fungi
115
Grain Twort Stain stain for? stain specifically for?
Staining of MICROORGANISMS STAIN Bacteria
116
Lendrum's Phloxine- Tartrazine Method stain for? stain specifically for?
Staining of MICROORGANISMS STAIN Viral Inclusions
117
Orcein stain for? stain specifically for?
Staining of MICROORGANISMS STAIN Hepatitis B Surface antigen
118
Rapid Giemsa stain for? stain specifically for?
Staining of MICROORGANISMS STAIN Blood and Bone marrow parasite (Leishmania, malaria and Trypanosome) Spirochete and other bacteria
119
Steiner and Modified Steiner stain for? stain specifically for?
Staining of MICROORGANISMS STAIN Spirochetes, Donovan Bodies, Fungi and Bacteria
120
Wade Fite Technique stain for? stain specifically for?
Staining of MICROORGANISMS STAIN Mycobacterium Leprae and Nocardia
121
Ziehl-Neelsen Method stain for? stain specifically for?
Ziehl-Neelsen Method Acid Fast Bacilli
122
Benzidine Nitroprusside Stain stain for? stain specifically for?
Staining of TISSUE PIGMENT and DEPOSITS Hemoglobin and Oxidase granules
123
Calcium Dye Lake Reaction stain for? stain specifically for?
Staining of TISSUE PIGMENT and DEPOSITS Skeletal system in Embryos and Fetuses
124
Gomori's Prussian Blue stain for? stain specifically for?
Staining of TISSUE PIGMENT and DEPOSITS Iron Pigments
125
Gomori's Aldehyde Fuchsin stain for? stain specifically for?
Staining of TISSUE PIGMENT and DEPOSITS Lipofuchsin
126
Mallory's Fuchsin Stain stain for? stain specifically for?
Staining of TISSUE PIGMENT and DEPOSITS Hemofuchsin
127
Masson Fontana Technique stain for? stain specifically for?
Staining of TISSUE PIGMENT and DEPOSITS Melanin & Argentaffin granules
128
Modified Fouchet's Technique stain for? stain specifically for?
Staining of TISSUE PIGMENT and DEPOSITS Liver Bile Pigment
129
stain for? stain specifically for?
Staining of TISSUE PIGMENT and DEPOSITS
130
Lindquist's Modified Rhodamine Technique stain for? stain specifically for?
Staining of TISSUE PIGMENT and DEPOSITS Copper
131
Perl's Prussian Blue stain for? stain specifically for?
Staining of TISSUE PIGMENT and DEPOSITS Hemosiderin
132
Schmorl's Ferric ferricyanide stain for? stain specifically for?
Staining of TISSUE PIGMENT and DEPOSITS Bile, Lipofuchsin, Melanin, Argetaffin cells, Thyroid Colloid
133
Turnbull's Blue Reaction for Ferrous Iron stain for? stain specifically for?
Staining of TISSUE PIGMENT and DEPOSITS Hemosiden
134
Von Kossas's Silver Nitrate Method stain for? stain specifically for?
Staining of TISSUE PIGMENT and DEPOSITS Demonstration of Calcium
135
Weigert's Pal Technique stain for? stain specifically for?
Staining of CENTRAL NERVOUS SYSTEM Myelin Sheath
136
Weil's Method stain for? stain specifically for?
Staining of CENTRAL NERVOUS SYSTEM Myelin Sheath
137
1. Kluver and Barrera Luxol Fast Blue stain 2. Luxol Fast Blue stain- H and E Stain 3.Luxol Fast Blue- Hematoxylin Stain stain for? stain specifically for?
Staining of CENTRAL NERVOUS SYSTEM Myelin
138
Bielschowsky Technique stain for? stain specifically for?
Staining of CENTRAL NERVOUS SYSTEM Neurons, Axons & Neurofibrils (CR) Black on Grayish background
139
Bodian's Stain stain for? stain specifically for?
Staining of CENTRAL NERVOUS SYSTEM Nerve fiber and Nerve endings (CR) Black
140
Cajal's Gold Sublimate stain for? stain specifically for?
Staining of CENTRAL NERVOUS SYSTEM Astrocytes, Nerve cells and nerve fibers
141
Cresyl fast violet stain for? stain specifically for?
Staining of CENTRAL NERVOUS SYSTEM Nissle Granules Neurons
142
Modified Holzer's Method stain for? stain specifically for?
Staining of CENTRAL NERVOUS SYSTEM Astrocytic processes
143
1.Bodian's Stain 2.Sevier Munger Technique stain for? stain specifically for?
Staining of CENTRAL NERVOUS SYSTEM Neural Tissues Demonstration of neuritic plaques and neurofibrillary tangles for the diagnosis of Alzheimer's Disease
144
Schleifsteins stain for? stain specifically for?
Staining of CENTRAL NERVOUS SYSTEM Negri bodies (Rabies) (Deep Magenta)

Finals (20 decks)

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