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HISTOPATH- Lec- Special Stains Flashcards

1
Q
  1. Mallory’s Phosphotungstic Acid Hematoxylin
  2. Highman’s Congo Red
  3. Alkaline Congo Red
  4. Kraijan’s Amyloid Stain
  5. Methyl Violet-Crystal Violet Method
  6. Induced Flourescent Staining with Thiotlavine-T

The ff are staining for?

A

CONNECTIVE TISSUES STAINS

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2
Q

Mallory’s Phosphotungstic Acid Hematoxylin
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Amyloid

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3
Q

Highman’s Congo Red
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Amyloid

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4
Q

Alkaline Congo Red
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Amyloid

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5
Q

Kraijan’s Amyloid Stain
stain specifically for?

A

Amyloid

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6
Q

Methyl Violet-Crystal Violet Method
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Amyloid

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7
Q

Induced Flourescent Staining with
Thiotlavine-T
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Amyloid

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8
Q
  1. Orcein (Taenzer-Unna)
  2. Weigert’s Elastic Tissue Stain
    The ff are staining for?
A

Staining of CONNECTIVE TISSUES STAINS

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9
Q

Orcein (Taenzer-Unna)
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Elastic Fibers

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10
Q

Weigert’s Elastic Tissue Stain
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Elastic Fiber

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11
Q

Kraijan’s Technique
staining of?

A

CONNECTIVE TISSUES STAINS

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12
Q

Kraijan’s Technique
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Elastic Fiber, Fibrin, Amyloid

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13
Q

Van Gieson (Acid-Fuchsin-Picric Acid)
staining for?

A

CONNECTIVE TISSUES STAINS

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14
Q

Van Gieson (Acid-Fuchsin-Picric Acid)
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Collagen

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15
Q

Masson’s Trichrome stain
staining for?

A

CONNECTIVE TISSUES STAINS

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16
Q

Masson’s Trichrome stain
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Collagen Fibers

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17
Q

Gomori’s Silver Impregnation
staining for?

A

CONNECTIVE TISSUE STAIN

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18
Q

Gomori’s Silver Impregnation
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Reticulin Fibers

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19
Q

Best known as an indicator

A

Congo Red

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20
Q

Congo Red
staining for?

A

CONNECTIVE TISSUE STAIN

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21
Q

Congo Red
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Elastic tissues, amyloid, myelin

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22
Q

Crystal Violet
staining for?

A

CONNECTIVE TISSUE STAIN

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23
Q

Crystal Violet
Staining of CONNECTIVE TISSUES STAINS
stain specifically for?

A

Amyloid (frozen section)
Platelets in blood

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24
Q

Borohyde Periodic Shiff
staining for?

A

Staining of FATS and LIPIDS STAINS

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25
Q

Borohyde Periodic Shiff
Staining of FATS and LIPIDS STAINS
stain specifically for?

A

Gangliosides

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26
Q

Nike Blue Sulfate
staining of?

A

Staining of FATS and LIPIDS STAINS

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27
Q

Nike Blue Sulfate
Staining of FATS and LIPIDS STAINS
stain specifically for?

A

Neutral Fats
Cholestrin Esters
and Fatty Acids
Cerebrosides

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28
Q

Oil Red O Method Dextrin
staining for?

A

Staining of FATS and LIPIDS STAINS

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29
Q

Oil Red O Method Dextrin
Staining of FATS and LIPIDS STAINS
stain specifically for?

A

Fats

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30
Q

Osmic Acid Stain
staining for?

A

Staining of FATS and LIPIDS STAINS

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31
Q

Osmic Acid Stain
Staining of FATS and LIPIDS STAINS
stain specifically for?

A

Unsaturated fats

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32
Q

Sudan IV (Scharlach R)
staining for?

A

Staining of FATS and LIPIDS STAINS

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33
Q

Sudan IV (Scharlach R)
Staining of FATS and LIPIDS STAINS
stain specifically for?

A

Triglycerides

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34
Q

Toluidine Blue Acetone Method
staining for?

A

Staining of FATS and LIPIDS STAINS

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35
Q

Toluidine Blue Acetone Method
Staining of FATS and LIPIDS STAINS
stain specifically for?

A

Sulfatide Deposits

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36
Q

Acridine Orange Flourescent (Masson Stain)
staining for?

A

Staining of NUCLEIC ACID

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37
Q

Acridine Orange Flourescent (Masson Stain)
Staining of NUCLEIC ACID
stain specifically for?

A

RNA and DNA

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38
Q

Methyl Green-Pyronin
stain for?

A

Staining of NUCLEIC ACID

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39
Q

Methyl Green-Pyronin
Staining of NUCLEIC ACID
stain specifically for?

A

RNA and DNA

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40
Q

Most reliable and specific histochemical staining technique for DNA

A

Fuelgen’s Technique

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41
Q

Fuelgen’s Technique
staining for?

A

Staining of NUCLEIC ACID

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42
Q

Fuelgen’s Technique
Staining of NUCLEIC ACID
stain specifically for?

A

DNA

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43
Q

Heidenhain’s Iron Hematoxylin
staining for?

A

Staining of MUSCLE and BONE STAIN

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44
Q

Heidenhain’s Iron Hematoxylin
Staining of MUSCLE and BONE STAIN
stain specifically for?

A

Muscle striations
Mitochondria
Myelin
Chromatin

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45
Q

Lissamine Fast-Red
stain for?

A

Staining of MUSCLE and BONE STAIN

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46
Q

Lissamine Fast-Red
Staining of MUSCLE and BONE STAIN
stain specifically for?

A

Muscle and Bones

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47
Q

Tartrazine Method
stain for?

A

Staining of MUSCLE and BONE STAIN

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48
Q

Tartrazine Method
Staining of MUSCLE and BONE STAIN
stain specifically for?

A

Muscle and Bones

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49
Q

Mallory’s Phosphotungstic Acid Hematoxylin
stain for?

A

Staining of MUSCLE and BONE STAIN

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50
Q

Mallory’s Phosphotungstic Acid Hematoxylin
Staining of MUSCLE and BONE STAIN
stain specifically for?

A

Muscle
Neuroglia
Myelin
Collagen

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51
Q

Modified Gomori’s Trichrome
stain for?

A

Staining of MUSCLE and BONE STAIN

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52
Q

Modified Gomori’s Trichrome
Staining of MUSCLE and BONE STAIN
stain specifically for?

A

Muscle Fibers and Collagen

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53
Q

Schnorl’s Picro Thionine Method
stain for?

A

Staining of MUSCLE and BONE STAIN

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54
Q

Schnorl’s Picro Thionine Method
Staining of MUSCLE and BONE STAIN
stain specifically for?

A

Bones (lacunae, canaliculi and bone matrix)

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55
Q

Rapid Toluidine-Eosin Stain
stain for?

A

Staining of BONE MARROW and BLOOD ELEMENTS

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56
Q

Rapid Toluidine-Eosin Stain
Staining of BONE MARROW and BLOOD ELEMENTS
stain specifically for?

A

Glycol Methcrylate section

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57
Q

Peroxidase reaction
stain for?

A

Staining of BONE MARROW and BLOOD ELEMENTS

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58
Q

Peroxidase reaction
Staining of BONE MARROW and BLOOD ELEMENTS
specifically for?

A

Myeloid cells

59
Q

Wright’s Giemsa Jenner
staining for?

A

Staining of BONE MARROW and BLOOD ELEMENTS

60
Q

Wright’s Giemsa Jenner
Staining of BONE MARROW and BLOOD ELEMENTS
specifically for?

A

Cytoplasmic granules

61
Q

Neutral Red
stain for?

A

Staining of BONE MARROW and BLOOD ELEMENTS

62
Q

Neutral Red
Staining of BONE MARROW and BLOOD ELEMENTS
specifically for?

A

Cell granules
Vacuoles of phagocytic cells

63
Q

Alpha Naphthyl Acetate
stain for?

A

Staining of ENZYMES

64
Q

Alpha Naphthyl Acetate
Staining of ENZYMES
specifically for?

A

Non-specific esterases

65
Q

Gomori Calcium Method
stain for?

A

Staining of ENZYMES

66
Q

Gomori Calcium Method
Staining of ENZYMES
specifically for?

A

Alkaline Phosphatase

67
Q

Gomori Dead Method
stain for?

A

Staining of ENZYMES

68
Q

Gomori Dead Method
Staining of ENZYMES
specifically for?

A

Acid Phosphatase

69
Q

Lead Method (Wachstein and Meisel)
stain for?

A

Staining of ENZYMES

70
Q

Lead Method (Wachstein and Meisel)
Staining of ENZYMES
specifically for?

A

5’-nucleotidase

71
Q

Indoxyl Acetate Method (Holt and Withers)
stain for?

A

Staining of ENZYMES

72
Q

Indoxyl Acetate Method (Holt and Withers)
Staining of ENZYMES
specifically for?

A

Esterases Activity

73
Q

Tetrazolium Method
stain for?

A

Staining of ENZYMES

74
Q

Tetrazolium Method
Staining of ENZYMES
specifically for?

A

Monoamine oxidase activity

75
Q

Alcian Blue Technique
stain for?

A

Staining of CARBOHYDRATES STAIN

76
Q

Alcian Blue Technique
Staining of CARBOHYDRATES STAIN
specifically for?

A

Most popular method for
demonstration of ACID MUCINS

77
Q

Combined Alcian Blue
stain for?

A

Staining of CARBOHYDRATES STAIN

78
Q

Combined Alcian Blue
Staining of CARBOHYDRATES STAIN
specifically for?

A

Demonstration of Mucins
Separating acid mucin and neutral mucin

79
Q

Best Carmine
stain for?

A

Staining of CARBOHYDRATES STAIN

80
Q

Best Carmine
Staining of CARBOHYDRATES STAIN
specifically for?

A

Fibrin
Glycogen
Mast cell granules
Mucin

81
Q

Flourescent Acridine Orange
stain for?

A

Staining of CARBOHYDRATES STAIN

82
Q

Flourescent Acridine Orange
Staining of CARBOHYDRATES STAIN
specifically for?

A

Acid mucopolysaccaride

83
Q

Flourescent Acridine Orange
-Can only last for _____ once the section is mounted

84
Q

Fresh frozen Azure-A
stain for?

A

Staining of CARBOHYDRATES STAIN

85
Q

Fresh frozen Azure-A
Staining of CARBOHYDRATES STAIN
specifically for?

A

Glycosaminoglycans

86
Q

Hale’s Dialyzed Iron Technique (colloidal)
stain for?

A

Staining of CARBOHYDRATES STAIN

87
Q

Hale’s Dialyzed Iron Technique (colloidal)
Staining of CARBOHYDRATES STAIN
specifically for?

A

Acid Mucins

88
Q

Langhan’s lodine Method or Carleton’s Method
stain for?

A

Staining of CARBOHYDRATES STAIN

89
Q

Langhan’s lodine Method or Carleton’s Method
Staining of CARBOHYDRATES STAIN
specifically for?

A

Not specific for Glycogen
May also stain Amyloid

90
Q

Metachromatic Toluidine Blue
stain for?

A

Staining of CARBOHYDRATES STAIN

91
Q

Metachromatic Toluidine Blue
Staining of CARBOHYDRATES STAIN
specifically for?

A

Glycosaminoglycans

92
Q

Mucicarmine stain
stain for?

A

Staining of CARBOHYDRATES STAIN

93
Q

Mucicarmine stain
Staining of CARBOHYDRATES STAIN
specifically for?

94
Q

Periodic Acid Shiff/ PAS
stain for?

A

Staining of CARBOHYDRATES STAIN

95
Q

Periodic Acid Shiff/ PAS
Staining of CARBOHYDRATES STAIN
specifically for?

A

Glycogen
Glomerular basement membrane
(+) Magenta

96
Q

PAS with Diastase
stain for?

A

Staining of CARBOHYDRATES STAIN

97
Q

PAS with Diastase
Staining of CARBOHYDRATES STAIN
specifically for?

98
Q

Southgate’s Mucicarmine Technique
stain for?

A

Staining of CARBOHYDRATES STAIN

99
Q

Southgate’s Mucicarmine Technique
Staining of CARBOHYDRATES STAIN
specifically for?

A

Encapsulated fungi like cryptococcus neoformans

100
Q

Toluidine Blue
staining for?

A

Staining of MICROORGANISMS STAIN

101
Q

Toluidine Blue
Staining of MICROORGANISMS STAIN
specifically for?

A

Helicobacter

102
Q

Cresyl Violet Acetate Method
stain for?

A

Staining of MICROORGANISMS STAIN

103
Q

Cresyl Violet Acetate Method
Staining of MICROORGANISMS STAIN
specifically for?

A

Helicobacter

104
Q

Levaditi’s Stain
stain for?

A

Staining of MICROORGANISMS STAIN

105
Q

Levaditi’s Stain
Staining of MICROORGANISMS STAIN
specifically for?

A

Spirochetes

106
Q

Auramine-Rhodamine Flourescent Method

A

Staining of MICROORGANISMS STAIN

107
Q

Auramine-Rhodamine Flourescent Method
Staining of MICROORGANISMS STAIN
specifically for?

A

Mycobacteria

108
Q

Warthin-Starry Method
stain for?

A

Staining of MICROORGANISMS STAIN

109
Q

Warthin-Starry Method
Staining of MICROORGANISMS STAIN
specifically for?

A

Spirochetes

110
Q

Brown and Brenn
stain for?

A

Staining of MICROORGANISMS STAIN

111
Q

Brown and Brenn
Staining of MICROORGANISMS STAIN
Specifically for?

112
Q

Dieterle Stain
stain for?

A

Staining of MICROORGANISMS STAIN

113
Q

Dieterle Stain
Staining of MICROORGANISMS STAIN
specifically for?

A

Legionella pheumophilla

114
Q

Grocott Methamine Silver
stain for?
stain specifically for?

A

Staining of MICROORGANISMS STAIN
Fungi

115
Q

Grain Twort Stain
stain for?
stain specifically for?

A

Staining of MICROORGANISMS STAIN
Bacteria

116
Q

Lendrum’s Phloxine- Tartrazine Method
stain for?
stain specifically for?

A

Staining of MICROORGANISMS STAIN
Viral Inclusions

117
Q

Orcein
stain for?
stain specifically for?

A

Staining of MICROORGANISMS STAIN
Hepatitis B Surface antigen

118
Q

Rapid Giemsa
stain for?
stain specifically for?

A

Staining of MICROORGANISMS STAIN
Blood and Bone marrow parasite
(Leishmania, malaria and Trypanosome)
Spirochete and other bacteria

119
Q

Steiner and Modified Steiner
stain for?
stain specifically for?

A

Staining of MICROORGANISMS STAIN
Spirochetes, Donovan Bodies, Fungi and Bacteria

120
Q

Wade Fite Technique
stain for?
stain specifically for?

A

Staining of MICROORGANISMS STAIN
Mycobacterium Leprae and Nocardia

121
Q

Ziehl-Neelsen Method
stain for?
stain specifically for?

A

Ziehl-Neelsen Method
Acid Fast Bacilli

122
Q

Benzidine Nitroprusside Stain
stain for?
stain specifically for?

A

Staining of TISSUE PIGMENT and DEPOSITS
Hemoglobin and Oxidase granules

123
Q

Calcium Dye Lake Reaction
stain for?
stain specifically for?

A

Staining of TISSUE PIGMENT and DEPOSITS
Skeletal system in Embryos and Fetuses

124
Q

Gomori’s Prussian Blue
stain for?
stain specifically for?

A

Staining of TISSUE PIGMENT and DEPOSITS
Iron Pigments

125
Q

Gomori’s Aldehyde Fuchsin
stain for?
stain specifically for?

A

Staining of TISSUE PIGMENT and DEPOSITS
Lipofuchsin

126
Q

Mallory’s Fuchsin Stain
stain for?
stain specifically for?

A

Staining of TISSUE PIGMENT and DEPOSITS
Hemofuchsin

127
Q

Masson Fontana Technique
stain for?
stain specifically for?

A

Staining of TISSUE PIGMENT and DEPOSITS
Melanin & Argentaffin granules

128
Q

Modified Fouchet’s Technique
stain for?
stain specifically for?

A

Staining of TISSUE PIGMENT and DEPOSITS
Liver Bile Pigment

129
Q

stain for?
stain specifically for?

A

Staining of TISSUE PIGMENT and DEPOSITS

130
Q

Lindquist’s Modified Rhodamine Technique
stain for?
stain specifically for?

A

Staining of TISSUE PIGMENT and DEPOSITS
Copper

131
Q

Perl’s Prussian Blue
stain for?
stain specifically for?

A

Staining of TISSUE PIGMENT and DEPOSITS
Hemosiderin

132
Q

Schmorl’s Ferric ferricyanide
stain for?
stain specifically for?

A

Staining of TISSUE PIGMENT and DEPOSITS
Bile, Lipofuchsin, Melanin, Argetaffin cells, Thyroid
Colloid

133
Q

Turnbull’s Blue Reaction for Ferrous Iron
stain for?
stain specifically for?

A

Staining of TISSUE PIGMENT and DEPOSITS
Hemosiden

134
Q

Von Kossas’s Silver Nitrate Method
stain for?
stain specifically for?

A

Staining of TISSUE PIGMENT and DEPOSITS
Demonstration of Calcium

135
Q

Weigert’s Pal Technique
stain for?
stain specifically for?

A

Staining of CENTRAL NERVOUS SYSTEM
Myelin Sheath

136
Q

Weil’s Method
stain for?
stain specifically for?

A

Staining of CENTRAL NERVOUS SYSTEM
Myelin Sheath

137
Q
  1. Kluver and Barrera Luxol Fast Blue stain
  2. Luxol Fast Blue stain- H and E Stain
    3.Luxol Fast Blue- Hematoxylin Stain
    stain for?
    stain specifically for?
A

Staining of CENTRAL NERVOUS SYSTEM
Myelin

138
Q

Bielschowsky Technique
stain for?
stain specifically for?

A

Staining of CENTRAL NERVOUS SYSTEM
Neurons, Axons & Neurofibrils (CR) Black on Grayish background

139
Q

Bodian’s Stain
stain for?
stain specifically for?

A

Staining of CENTRAL NERVOUS SYSTEM
Nerve fiber and Nerve endings (CR) Black

140
Q

Cajal’s Gold Sublimate
stain for?
stain specifically for?

A

Staining of CENTRAL NERVOUS SYSTEM
Astrocytes, Nerve cells and nerve fibers

141
Q

Cresyl fast violet
stain for?
stain specifically for?

A

Staining of CENTRAL NERVOUS SYSTEM
Nissle Granules
Neurons

142
Q

Modified Holzer’s Method
stain for?
stain specifically for?

A

Staining of CENTRAL NERVOUS SYSTEM
Astrocytic processes

143
Q

1.Bodian’s Stain
2.Sevier Munger Technique
stain for?
stain specifically for?

A

Staining of CENTRAL NERVOUS SYSTEM
Neural Tissues
Demonstration of neuritic plaques and
neurofibrillary tangles for the
diagnosis of Alzheimer’s Disease

144
Q

Schleifsteins
stain for?
stain specifically for?

A

Staining of CENTRAL NERVOUS SYSTEM
Negri bodies (Rabies) (Deep Magenta)

Finals (20 decks)

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