Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Finals > HISTOPATH- Lec-Gynecological Specimen > Flashcards

HISTOPATH- Lec-Gynecological Specimen Flashcards

1
Q

Anatomic Sites for Cytologic Samples

A
  • Upper (Proximal) Third of the Vaginal Wall
  • Ectocervix
  • Endocervix
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Anatomic Sites for Cytologic Samples:
Ideal for studying hormonal status, evaluation of inflammatory
conditions, classification of the normal flora and rarely detection of
malignant vaginal lesions

A

Upper (Proximal) Third of the Vaginal Wall

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Anatomic Sites for Cytologic Samples:
most common for cancer screening

use of Ayre’s spatula (can reach the T-zone)

Histology: Stratified Squamous Non-Keratinizing

A

Ectocervix

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Anatomic Sites for Cytologic Samples:
for detection of endocervical lesions, intrauterine lesions

Histology: Simple Columnar Epithelium

A

Endocervix

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Ectocervix histology?

A

Stratified Squamous Non-Keratinizing

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Endocervix histology?

A

Simple Columnar Epithelium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Cytologic Collection and Preparation
Materials Used for Conventional Paps Smear:

A

Endocervical Brush
Vaginal Scrape
Lateral Vaginal Scrape
Four Quadrant Vaginal Scrape
Vulvar Scrape

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Materials Used for Conventional Paps Smear:
-for endocervical canal

A

Endocervical Brush

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Materials Used for Conventional Paps Smear:
- for patients with hysterectomy

A

Vaginal Scrape

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Materials Used for Conventional Paps Smear:
-for Hormonal Cytology

A

Lateral Vaginal Scrape

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Materials Used for Conventional Paps Smear:
- for localization of vaginal adenosis

A

Four Quadrant Vaginal Scrape

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Materials Used for Conventional Paps Smear:
- for detection of herpetic lesions or carcinoma

A

Vulvar Scrape

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Equipment for Vaginal, Endocervical and
Endometrial Aspirations

A

Glass Pipet and Rubber Bulb
Ayre’s Spatula
Laryngeal Cannula attached to 10cc syringe

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

-for vaginal aspiration
6-8 inches x ¼ inches

A

Glass Pipet and Rubber Bulb

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Glass Pipet and Rubber Bulb size

A

6-8 inches x ¼ inches

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

swab smear

A

Ayre’s Spatula

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

-for endocervical/endometrial aspiration

A

Laryngeal Cannula attached to 10cc syringe

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Antiseptic used

A

Zephiran

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Papanicolau Smear and Stain
Developed by:

A

George Nicolas Papanicolau

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Papanicolau Smear and Stain
Screening Test for:

A

Cervical Cancer

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Screening Test for: Cervical Cancer

A

Papanicolau Smear and Stain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Papanicolau Smear and Stain
USES:

A

Determine certain infections (STD)

Determine ovarian function (Hormonal Cytology)

Asses infertility

Medico Legal Examination of Sexual Assault

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Pap’s Stain:
nuclear stain (Primary Stain)

A

Harris Hematoxylin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Pap’s Stain ;
stains the cytoplasm of superficial cells (Counterstain)
-strong affinity for mature cells

A

Orange Green-6 (OG 6)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

Pap’s Stain:
stains the cytoplasm of Parabasal cells and
intermediate cells (Counterstain)
-strong affinity for immature cells

A

Eosin Azure-50 (EA-50)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

Pap’s stain: strong affinity for mature cells

A

Orange Green-6 (OG 6)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

Pap’s stain: -strong affinity for immature cells

A

Eosin Azure-50 (EA-50)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

Pap’s stain:

A

Harris Hematoxylin
Orange Green-6 (OG 6)
Eosin Azure-50 (EA-50)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

PAP Stain Result:
Nucleus:
= Vesicular nucleus:
= pyknotic nucleus:

A

Vesicular nucleus: blue
Pyknotic nucleus: dark blue to black

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

PAP Stain Result:
Cytoplasm:
OG-6:
EA 36 to 50:

A

OG-6: Orange with a hint of green
EA 36 to 50: Olive Green with a hint of brown and red

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

PAP Stain Result:
Bacteria:

A

dark blue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

PAP Stain Result:
Mycelia:

A

violet

33
Q

PAP Stain Result:
Trichomonas vaginalis:

A

pale greenish blue blob of cytoplasm

34
Q

PAP Stain Procedure

A
  1. Fix with 95% ethanol
  2. Hematoxylin
  3. Differentiate with Acid Alcohol the wash with water
  4. Ammonia water then wash
  5. OG6
  6. 95% ethanol (washing) (2 changes)
  7. EA 50 or 36
  8. Dehydration
  9. Xylol
  10. Mount
  11. Label
35
Q

Cells Found in Cervicovaginal Smears

A

Superficial Cells (30-60u)
Intermediate Cells (20-30um)

36
Q

most mature, polygonal cells with Dark pyknotic nuclei
Cells that exhibits “TRUE ACIDOPHILIA” characteristics of vaginal cell under the influence of ESTROGEN

A

Superficial Cells (30-60u)

37
Q

influence desquamation of Superficial cells

A

ESTROGEN

38
Q

influence desquamation of
intermediate cells

A

PROGESTERONE

39
Q

most predominant during menopause

A

ESTRONE (E1)

40
Q

most predominant during reproductive years

A

ESTRADIOL (E2)

41
Q

plenty but least potent

A

ESTRIOL (E3)

42
Q

Medium cells, polyhedral with basophilic & vacuolated cytoplasm

A

Intermediate Cells (20-30um)

43
Q

Intermediate Cells (20-30um)

A

Navicular Cells
Parabasal Cells
Pregnancy Cells

44
Q

found in the latter half of
menstrual cycle, menopause and during
pregnancy (presence suggest
Progesterone-Estrogen Effect)

A

Navicular cells

45
Q

with a tendency to fold or curl on edges

A

Boat shaped cells

46
Q

-fried egg appearance/ sunny side-up

-Round oval nucleus/cells

Normally found in: abortion, after
menopause, after childbirth, 2 week of Age of
puberty

A

Parabasal Cells (15-25um)

47
Q

Parabasal Cells (15-25um)
Normally found in:

A

abortion, after menopause, after childbirth, 2 week of Age of puberty

48
Q

Small, round slightly oval cells, with relatively
large nucleus that occupying half or more of
the cell volume

A

*Basal Cells (13-20um)

49
Q

*Basal Cell’s cytoplasm is

A

strongly basophilic

50
Q

Basal cells are Found in vaginal smears only ______ and _____

A

before pregnancy and after menopause

51
Q

Nucleus pushed at periphery with double walled boundary appearance

A

Pregnancy Cells

52
Q

Round oval shaped cell appearing boat cells with translucent basophilic cytoplasm due to glycogen
accumulation

A

Pregnancy Cells

53
Q

-found during menstrual period (in groups)

A

Endometrial Cells

54
Q

If seen in post- menopausal woman indicate a
possible endometrial carcinoma or endometrial
hyperplasia

A

Endometrial Cells

55
Q

Endometrial Cells If seen in post- menopausal woman indicate a possible?

A

endometrial carcinoma or endometrial hyperplasia

56
Q

Similar to parabasal cells but less basophilic

A

Endometrial Cells

57
Q

Honey-comb appearance

A

Endocervical/Glandular Cells

58
Q

Occurs in groups and strips of 3 or more cells

A

Endocervical/Glandular Cells

59
Q

Slightly cylindrical appearance/ columnar epithelial cells

A

Endocervical/Glandular Cells

60
Q

Normal vaginal flora

A

Doderlain Bacillus/Lactobacillus Acidophilus

61
Q

Doderlain Bacillus/Lactobacillus Acidophilus increased in?

A

L- last trimester of pregnancy

I- infection

E- estrogen deficiency

D- diabetic patients

62
Q

** PAPS smear should be done after______of LMP

A

10 days

63
Q

yeast cell that is commonly seen in patients with
diabetes, taking contraceptive and with
immunocompromise state

A

Candida Albicans

64
Q

STD
Pear-shaped organism
Greenish blue appearance in PAPs SMEAR
Eccentric dark nucleus

A

Trichomonas vaginalis

65
Q

May appear as cells surrounded by bacilli “CLUE CELLS”

A

Gardnerella vaginalis

66
Q

Cells that shows cytophatic effect of human papilloma virus
Nucleus: appears like “wrinkled prune” that is surrounded by a
perinuclear halo

A

Koilocytes

67
Q

Mucus on drying exhibits a fern or palm leaf pattern

A

Ferning (Arborization)

68
Q

Basis for the diagnosis of Early Pregnancy

A

Ferning (Arborization)

69
Q

(+) Ferning: High Persistent
______ Effect in the absence of ______

A

Estrogen; progesterone

70
Q

-assess the percentage of cells coming from the main layers of the epithelium

A

Maturation Index (M.I)

71
Q
  • Assess the percentage of cells appearance that stains pink-orange to red with Pap’s
A

Acidophilic/Eosinophilic Index (A.I)

72
Q

-assess percentage of cells with shrunken, dark, small, structureless nucleus

A

Pyknotic Index (P.I)

73
Q

Reporting Cancer Cytology: Class I

A

Absence of atypical cytologic picture

74
Q

Reporting Cancer Cytology: CLASS II

A

Atypical cytologic picture present but no evidence of
malignancy

75
Q

Reporting Cancer Cytology: Class III

A

Cytologic picture suggestive but not conclusive to
malignancy

76
Q

Reporting Cancer Cytology: Class IV

A

Cytologic picture strongly suggestive of malignancy

77
Q

Reporting Cancer Cytology: Class V

A

Cytologic picture conclusive of malignancy

78
Q

Classification of PAP Test Results

A

See handouts

Finals (20 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions