AUBF-Lec-HCG Flashcards
Meaning of HCG
Human Chorionic Gonadotropin
HCG A.K.A.
Pregnancy hormone
HCG is Produced by the ________ cells of the placenta
syncytiotrophoblast
HCG: Peaks during ________ of pregnancy (Increased in BLOOD, URINE and AMNIOTIC FLUID)
1st trimester
Peaks during 1st trimester of pregnancy (increased in (3)
BLOOD, URINE and AMNIOTIC FLUID
HCG is Composed of 2 subunits:
ALPHA: hCG, LH, FSH, TSH
BETA: Confers specificity for hCG
HOME-BASED HCG PREGNANCY TEST KIT:
Principle:
Specimen:
Cut-off point:
Anti-hCG source:
Principle: Enzyme immunoassay
Specimen: 1st morning urine
Cut-off point: 25 mIU/mL
Anti-hCG source: RABBIT
Quali or Quanti: HOME-BASED HCG PREGNANCY TEST KIT
Qualitative
this blood test measures the specific HCG hormone in your blood in international units per liter (IU/L). The level of HCG helps determine the age of the fetus
Beta hCG
Quantitative hCG
Beta hCG
HCG BIOASSAY (BIOLOGICAL PREGNANCY TESTS):
Hogben:
Animal Used:
Mode of Injection:
Positive Result:
Sensitivity:
Female Frog
Lymph sac (urine)
Oogenesis
75-100 IU/mL
HCG BIOASSAY (BIOLOGICAL PREGNANCY TESTS):
Galli-Mainini
Animal Used:
Mode of Injection:
Positive Result:
Sensitivity:
Male Frog
Subcutaneous (urine/serum)
Spermatogenesis
Variable
HCG BIOASSAY (BIOLOGICAL PREGNANCY TESTS):
Friedmann/Hoffmann
Animal Used:
Mode of Injection:
Positive Result:
Sensitivity:
Virgin female rabbit
Marginal ear vein
Corpora lutea & corpora hemorrhagica
10-15 IU/mL
HCG BIOASSAY (BIOLOGICAL PREGNANCY TESTS):
Ascheim-Zondek
Animal Used:
Mode of Injection:
Positive Result:
Sensitivity:
Immature female mice
Subcutaneous (urine)
Formation of hemorrhagic follicles & corpora lutea
1-6 IU/mL
HCG BIOASSAY (BIOLOGICAL PREGNANCY TESTS):
Frank-Berman
Animal Used:
Mode of Injection:
Positive Result:
Sensitivity:
Immature female rats
Subcutaneous (urine)
Ovarian hyperemia
1 IU/mL