Hematopoiesis - Lough

Question 1

What is the difference between red and yellow bone marrow?

Answer 1

Red marrow is hematopoietic and in the flat bones

Yellow marrow is fatty and in the long bones.

Question 2

What are multipotent master stem cells?

Answer 2

progenitors of all the cell types in bone marrow

Question 3

What varieties of stem cells can be found in bone marrow? What do they produce?

Answer 3

Multipotent master stem cells- produce all bone marrow cells

Mesenchymal stem cells- produce bone, endothelial cells

Hematopoietic stem cells- produce all blood cell progenitors

Question 4

How are bone marrow cells sorted?

Answer 4

They are sorted by clusters of differentiation using FACS

Question 5

How does FACS work?

Answer 5

Cells are incubated with antibodies that have a charged fluorophore. When they go through the machine, they are counted by the color emitted by the fluorophore and sorted into groups by the charge.

Question 6

What three classes of cell are considered myeloid?

Answer 6

Granulocytes
Monocytes
Megakaryocytes

Question 7

What is a colony forming unit?

What are some examples?

Answer 7

A chemokine/cytokine that activates growth of cells

erythropoietin
thrombopoietin
G-CSF (Granulocyte colony stimulating factor)
etc

Question 8

What are the progenitors of the following cells:

Basophils
Neutrophils
Erythrocytes
Eosinophils

Answer 8

Basophils-Myeloblasts
Neutrophils-Myeloblasts
Erythrocytes- Proerythroblasts
Eosinophils-Myeloblasts