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RUSVM Clinical Pathology > Hematology > Flashcards

Hematology Flashcards

1
Q

Packed Cell Volume

A

Percentage of whole blood composed of erythrocytes - manual

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2
Q

Reticulate count of a regenerative anemia

A

> 60,000 ul

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3
Q

Hematocrit

A

Percentage of whole blood composed of erythrocytes calculated by an electronic cell counter

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4
Q

Differential Nucleated Cell Count

A

Minimum of 100 nucleated cells are counted within a counting area and classified as: segemented neutrophils, band neutrophils, lymphocytes, monocytes, eosinophils, basophils, nucleated RBC, other

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5
Q

Normal size of cat, horse and cow RBC

A

39-52 fl

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6
Q

Normal size of a dog RBC

A

60-72 fl

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7
Q

Light Scatter Measurement (Flow)

A

Cells passed through a flow cell that is intersected by a focused laser beam. Light scattered in angles correlates with cellular properties leading to ability to differentiate cell types

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8
Q

Refractometer

A

Estimates the concentration of solute in fluid, since solute bends light passing through the fluid proportionate to the solute concentration

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9
Q

Techniques for getting a complete blood count

A

Direct Measurements

Microscopic Procedures

Calculations

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10
Q

Hematologic techniques performed with automated instrumentation:

A

Cell particle counting and sizing

Spectrophotometry

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11
Q

Electronic Cell Counting

A

Cells are suspended in an electrolyte medium that conducts electricity, deflections in current are proportional to the size of the cell

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12
Q

Yellow pigmentation of plasma suggests what in small animals

A

Suggestive of icterus - increased bilirubin concentration in the blood

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13
Q

Yellow pigmentation of plasma in large animals is suggestive of

A

Normal Plasma - may be due to carotene pigments associated with diets

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14
Q

Reticulate count of a poorly regenerative anemia

A

10,000-60,000ul

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15
Q

White or opaque plasma may be due to

A

postprandial collection or may be due to disease associated with abnormalities in lipid metabolism

___________________________

Often due to not fasting before blood draw

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16
Q

Reticulocyte

A

Immature erythrocytes that still have organelles for protein synthesis and aerobic metabolism

17
Q

Mean Cell Hemoglobin Concentration =

A

MCHC = Hgb / (PCV% x 100)

18
Q

Describe reticulocyte maturation in cats

A

Aggregate reticulocytes become punctate reticulocytes in about 12 hours. Punctates persist for 12 days.

______________________

Punctates are not polychromatic with wrights stain and not counted in reticulocyte count.

19
Q

Refractometers are used in clinical practice to estimate

A

Plasma Protein
Urine Specific Gravity

20
Q

Total Nucleated Cell Count (Total Leukocyte Concentration)

A

Detects all nuclei in solutions from which red blood cells have been removed by lysis

Primarily used to calculate the concentration of specific leukocyte types - after differentiated cell count

21
Q

Hematocrit =

A

HCT = MCV x (RBC / 10)

22
Q

Species that does not release reticulocytes

A

Horses

23
Q

There should be how many platelets per oil immersion field?

A

6-10

24
Q

Decreases in MCHC may be due to

A

Iron deficiency

25
Q

Red coloration of plasma is due to the presence of

A

Hemoglobin

26
Q

Reticulate Count of a non regenerative anemia

A

0-10,000ul

27
Q

Red Cell Distribution Width (RDW)

A

Describes the relative width of the size distribution curve

28
Q

When stained with Wrights Stain - reticulocytes are _____________ RBCs

A

Polychromatic

29
Q

Reticulocyte concentration can be measured by

A

Flow technology

Manually

30
Q

Increases in MCHC are indicative of

A

Artifacts. Due to hemolysis, lipemia or presence of Heinz bodies

31
Q

In dogs, reticulocytes mature in ________ hours

A

24-48 hours

32
Q

Buffy coat consists of

A

Leukocytes

Nucleated Erythrocytes

Platelets

RUSVM Clinical Pathology (22 decks)

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