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RUSVM Clinical Pathology > Basic Hematologic Techniques > Flashcards

Basic Hematologic Techniques Flashcards

1
Q

Describe the process of getting a PCV

A

Fill microhematocrit tube 70-90%

Plug one end with clay

Place in centrifuge

Remove from centrifuge

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2
Q

How does a refractometer work?

A

Solute bends light passing through the fluid, refraction is proportionate to teh solute concentration

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3
Q

Solutes that will interfere with refractometer readings (artificially increase TP)

A

Lipemia

Urea

Glucose

Cholesterol

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4
Q

If PCV and TP are proportionally increased then the patient

A

is dehydrated

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5
Q

If the PCV and TP are proportionally decreased then the patient

A

has blood loss

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6
Q

Two major constituents of Total Protein

A

Albumin

Globulin

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7
Q

Increased TP, Globulin and Albumin

A

Dehydration

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8
Q

Increased TP and Globulin

A

Inflammation or Neoplasia

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9
Q

Decreased TP, Albumin and Globulin

A

Blood Loss

Protein Losing Enteropathy

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10
Q

Decreased TP and Albumin

A

Protein Losing Nephropathy

Liver Failure

Vaculitis

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11
Q

Decreased TP and Globulin

A

FPT

SCIDS

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12
Q

Stained blood films are essential for

A

Determining concentration of individual leukocyte types

Evaluating morphologic abnormalities of leukocytes, erythrocytes and platelets

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13
Q

Stains used to stain a blood slide

A

Wright Stain

Wright Giemsa Stain

Diff Quik

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14
Q

Systemic Blood Film Evaluation steps

A
  1. 10x lens - Scan feathered edge
  2. 10x lens - Examine monolayer
  3. 100x lens - Evaluate monolayer
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15
Q

What should you be looking for on 10x when looking at the feathered edge?

A

Platelet clumps

Organisms

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16
Q

What should you be looking for on 10x looking at the monolayer?

A

Estimating RBC and WBC count

17
Q

On 100x looking at the monolayer what should you be doing?

A

Evaluating RBC, WBC (differentiating) and platelets

18
Q

RBC evaluation should involve

A

Size

Color

Shape

Arrangement

Inclusions/Organisms

19
Q

WBC evaluation should involve

A

Differential cell count

Leukocyte morphology

20
Q

Macroplatelets / Giant Platelets

A

Term used to describe platelets that are near the size of RBCs

RUSVM Clinical Pathology (22 decks)

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