Collection and Handling of Biological Samples

Question 1

Blood for biochemical profile should be analyzed in what time frame?

Answer 1

Blood should be allowed to clot for 15-30 minutes

Serum should be separated by centrifugation

Refrigerate serum or freeze if not being analyzed within 2 days

Question 2

Green Top

Answer 2

Heparin

Anticoagulant - inhibits thrombin

Contains fibrinogen

Uses: Chemistry, Avian/reptile CBC and chem, Plasma colloid oncotic pressure, measurement of electrolytes, specific tests

Question 3

Tiger Top (Red and Black)

Answer 3

Gel that promotes blood clot formation and separates cells from serum

Used for chemistry and serology

Question 4

Blue Top

Answer 4

Citrate

Anti-Coagulant, Ca Chelator

Use: Coag tests, PIVKA, Antithrombin, Coag Factor Analysis, Von Willebrands Factor Analysis

Question 5

Gray Top

Answer 5

Sodium Fluoride Oxalate

Anti-coagulant, Ca chelator

Inhibits glucose metabolism

Use: serial glucose, lactate and pyruvate

Question 6

Red Top Tube

Answer 6

No Anti-Coagulant

Blood is expected to clot

Serum used for biochemical profile and other tests

Question 7

Lavender Top

Answer 7

EDTA to K Salt

Anticoagulant and Ca2+ chelator

Preserves cell morphology

Uses: CBC, Fibrinogen, Reticulocyte count, Buffy coat analysis, fluid analysis, blood banks, coombs test, PCR, endogenous ACTH

Question 8

Blood for a CBC should be analyzed in what time frame

Answer 8

Within the hour of taking the sample

Question 9

Volume of blood needed for CBC and Biochemical Profile?

Answer 9

5 mL