Gram Negative Rods Flashcards
Bordet-Gengou agar, whooping cough
Bordetella pertussis
Poorly gram staining, silver stain, charcoal yeast agar, airconditioning
Legionella pneumophilia
Satellite phenomenon around S. Aureus
H. Influenzae
- Most common cause of acute epiglottitis
2. Treatment for epiglottitis
- H. Influenzae
2. Ceftriaxone
Damages ciliated cells; causes whooping
Tracheal cytotoxin (bordetella pertussis)
Stage of pertussis where bacterial load is highest
Catarrhal stage
Stage of pertussis where whooping is observed
Paroxysmal stage
Stage of pertussis where complications develop (pneumonia, seizures, encephalopathy)
Convalescent stage
Enriched chocolate agar, polyribitol phosphate capsule
Hemophilus influenza type B
Transmission via environmental water source; person to person transmission does not occur
Legionella pneumophila
Endotoxin is the sole virulence factor; cell replicates intracellularly
Legionella
Pontiac fever
Legionella
Atypical pneumonia with electrolyte imbalance (diarrhea, hyponatremia, proteinuria, confusion)
Legionella
Lactose fermenter, urease positive, produces a green/metallic sheen on EMB
E. Coli
Lactose fermenter, urease positive, ESBL
Klebsiella pneumoniae
Comma shaped, microaerophilic, skirrow’s agar
Campylobacter jejuni
Comma shaped, urease positive, skirrow’s agar
Helicobacter pylori
Motile, oxidase negative, H2S producer, nonlactose fermenting
Salmonella
Nonmotile, oxidase negative, H2S nonproducer, nonlactose fermenter
Shigella
Swarming, oxidase negative, H2S producer, urease positive
Proteus mirabilis
- Causative organism of Struvite stones
2. Composition of struvite
- Proteus mirabilis
2. Magnesium, ammonium, phosphate
Oxidase positive, H2S nonproducer, obligate aerobe
Pseudomonas aeruginosa
Causes watery diarrhea in E. Coli infection
HST and HLT toxins
Causes bloody diarrhea in E. Coli infection
Shiga-like verotoxin
Virulence factors: H antigen (flagellum), K antigen (capsule), O/somatic antigen (endotoxin), enterotoxins
E. Coli
Traveler’s diarrhea
ETEC
Watery diarrhea in children and developing countries
EPEC
Bloody diarrhea; can cause HUS
EHEC/STEC
Typing of EHEC
O157:H7
Bloody diarrhea without HUS
EIEC
Persistent watery diarrhea in the young and immunocompromised (HIV/AIDS)
EAEC
Salmonella that lives in human colon only
Salmonella typhi
Salmonella that lives in both humans and animals (poultry, eggs)
Salmonella enteritidis
Salmonella enterocolitis
S. Enteritidis, s. Typhimurium
Typhoid fever
S. Typhi
Chronic carrier state of S. Typhi
Gallbladder
Antigen in typhoid fever that allows for invasion in peyer’s patches
Vi capsular antigen
Salmonella choleraesuis septicemia:
- Common sites of seeding (3)
- Common patient populations
- Bone (osteomyelitis), lung (pneumonia), brain (meningitis)
- Px with sickle cell anemia, cancer
Ideal samples for typhoid fever during:
Week 1
Week 2
Week 3
Sample can be taken from __________ at any point during the illness
- Blood
- Urine
- Stool
Bone marrow
Pulse-fever disproportion, rose spots
Typhoid fever (S. Typhi)
Transmission of shigella (4 Fs)
Food, fingers, feces, flies
Most common cause of bacillary dysentery
Shigella sonnei (Duval’s bacillus)
Most severe type of dysentery; most common cause of epidemic dysentery
Shigella dysenteriae type 1
Group A: shigella dysenteriae
Type 1: shiga bacillus
Type 2: Schmitz bacillus
Group B: shigella flexneri
Flexner’s bacillus, Hiss and Russell’s bacillus
Group C: shigella boydii
Newcastle Manchester bacillus
Group D: shigella sonnei
Duval’s bacillus
Vibrio spp that lives in human colon only
Vibrio cholerae
Vibrio spp that lives in saltwater
V. Parahemolyticus, V. Vulnificus
Vibrio spp that causes cholera pandemics
Vibrio cholerae O1 biotype El Tor (cholera El Tor)
Cholera enterotoxin that acts via ADP ribosylation
Choleragen
Cholera virulence factor that causes attachment to the intestinal mucosa
Mucinase
Grows well in hot temperatures (42 C)
Campylobacter jejuni
Most common cause of bacterial gastroenteritis
Campylobacter jejuni
Associated diseases of campylobacter jejuni
Guillain barre syndrome
Reiter’s syndrome
Damages gastric goblet cells; produces ammonia from urea that neutralizes gastric acid
H. Pylori
Associated diseases of H. Pylori
Gastric CA, MALT lymphoma
4 antibiotics used in the tx of H. Pylori
Tetracycline, metronidazole, amoxicillin, clarithromycin
Necrotizing pneumonia with currant jelly sputum
Klebsiella pneumoniae
Most common cause of pneumonia in alcoholics
Klebsiella
Blue green pigment of pseudomonas
Pyocyanin
Causes the sweet grape-like odor of pseudomonas
Aminoacetophenone
Virulence factors responsible for necrotizing pneumonia in pseudomonas
Elastase and protease
Damages cilia and mucosal cells in pseudomonas infection
Pyocyanin
Causes hemoglobin breakdown in pseudomonas infection
Verdoglobin
Hot tub folliculitis, green nail syndrome, burn infections, contact lens keratitis
Pseudomonas aeruginosa
Most common cause of otitis externa, CSOM
Pseudomonas aeruginosa
Osteomyelitis and pubic osteomyelitis in IV drug users
Pseudomonas aeruginosa
Typhlitis, Shanghai fever, peritonitis in px undergoing peritoneal dialysis
Pseudomonas
Predominant anaerobe of the human colon
Bacteroides fragilis
Causes abdominal abscesses, peritonitis, or cerebral abscess with bowel perforation
Bacteroides fragilis