Glycolysis

Question 1

Pasteur effect concluded that the glucose stimulating factor is

Answer 1

Pi

Question 2

Location?

Answer 2

Anaerobic, cytoplasm

Question 3

RDS?

Answer 3

F-6-P > F-1,6 BisP via PFK-1

Question 4

NADH formed in which step?

Answer 4

G3PDH

Question 5

HK vs GK

Answer 5

HK:
- In all cells
- High affinity to glucose
- Inhibited by G-6-P
- Constitutive
GK:
- In liver/pancreas only
- Low affinity to glucose
- Inhibited by F-6-P
- Inducible(increases with insulin)

After a meal > HK inhibited, GK active

Question 6

PFK-2 regulation

Answer 6

Phosphorylated > active phosphatase
DePhosphorylated > active kinase

Question 7

T/F: PFK-1 is regulated by F-2,6BisP in all cells

Answer 7

False. Skeletal muscles PFK-1 is independent of F-2,6BisP. It depends on glucose flux

Question 8

Pyruvate kinase regulation

Answer 8

DePhosphorylated > active
Phosphorylated > inactive

Question 9

T/F: Glucagon/Hypoglycemia inhibits glycolysis in all tissues

Answer 9

False. Only in liver. It is normal in extra-hepatic tissues.