ETC

Question 1

Location of ETC

Answer 1

IMM

Question 2

Marker enzyme in OMM

Answer 2

Monoamine oxidase

Question 3

T/F: IMM permeable to almost everything

Answer 3

False. OMM is
IMM impermeable to almost everything

Question 4

Mitoplast

Answer 4

IMM + Matrix when OMM is removed by digitonin

Question 5

Submitochondrial particle

Answer 5

Membrane is inside out

Question 6

ANT(Adenine nucleotide transporter)

Answer 6

• In IMM
• Cytosol affinity to ADP
• Matrix affinity for ATP
• ADP goes to matrix to generate E

Question 7

NADH shuttles

Answer 7

G3P:
- DHAP > 3PG
- NADH > NAD+
- NADH > 2 ATP
Malate-Aspartate:
- OXAC > Malate
- NADH > NAD+
- NADH > 3 ATP

Question 8

All e- carriers can carry 2e- except

Answer 8

Cytochromes

Question 9

In which cytochrome does O2 > H2O

Answer 9

a3

Question 10

Complex names?

Answer 10

I: NADH DH
II: Succinate DH
III: Cyt C reductase(b, c1)
IV: Cyt C oxidase(a, a3)
V: ATP synthase

Question 11

Which are coupling sites(ox and phos), enough E for ATP synthesis, H+ enters IM space

Answer 11

I, III, IV

Question 12

Which theory explained need for tight membrane?

Answer 12

Chemiosmotic theory(re-entry of H+ to matrix through V drives ATP synthesis)

Question 13

F0 vs F1

Answer 13

F0:
- Flow of H+ into matrix
- In IMM
F1:
- Catalytic…ADP > ATP
- In matrix

Question 14

F0 and F1 linked by

Answer 14

Oligomyosin sensitivity conferring protein(OSCP)

Question 15

Uncouplers do what to V

Answer 15

Synthase > ATPase

Question 16

T/F: ETC defect by nuclear ot mt. DNA

Answer 16

True

Question 17

T/F: Respiratory quotient > 1…aerobic metabolism

Answer 17

False…RQ<1 = aerobic