Glucose Methodologies

Question 1

standard spx

Answer 1

serum

Question 2

PLASMA – can also be used and is _% lower compared to serum

Answer 2

5%

Question 3

WHOLE BLOOD – _lower than serum

Answer 3

10-15%

Question 4

capillary

fbs:
ogtt:

Answer 4

fbs: 7 mg/dL
ogtt: 20-70 mg/dL (20-25% higher than venous blood)

Question 5

– similar to capillary blood

Answer 5

ARTERIAL BLOOD

Question 6

same with serum and plasma

Answer 6

PERITONEAL FLUID

Question 7

CSF –

Answer 7

NR: 60 – 70% PG

Question 8

10% contamination of blood sample with 5% dextrose can increase PG by _ (falsely elevated PG)

Answer 8

500 mg/dL

Question 9

If delayed, centrifugation and aliquot it will cause _ to blood glucose

Answer 9

false dec

Question 10

anticoagulant for delayed separation of serum/plasma from cell

Answer 10

sodium fluoride (additive) + potassium oxalate (anticoag)

Question 11

inhibits glycolysis by inhibiting the enzyme _ and forming complex with _

Answer 11

NaF; enolase; magnesium

Question 12

Enolase requires _ to be activated

Answer 12

magnesium

Question 13

Required amount of NaF: _
Required amount of K2C2O4: _

Answer 13

Required amount of NaF: 2 mg NaF / ml blood
Required amount of K2C2O4: 2mg NaF / ml blood

Question 14

hemolyzed spx causes

Answer 14

Dilutional Effect causing FD

Question 15

STORAGE CONSIDERATION:

o Room Temperature:

o Refrigerator:

Answer 15

o Room Temperature (20-25 C):
7 mg/dL/hour decrease

o Refrigerator (4 C): 2 mg/dL/hour decrease

Question 16

Decrease rate occurs only in _

Answer 16

uncentrifuged clotted blood